We narrowed to 497 results for: staphylococcus

1. PX382 Staphylococcus pseudintermedius Cas9

   Plasmid

   #68319

   Purpose

   Staphylococcus pseudintermedius Cas9

   Depositor

   Feng Zhang

   Insert

   SpsCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX382 Staphylococcus pseudintermedius Cas9

   Plasmid

   #68319

   Purpose

   Staphylococcus pseudintermedius Cas9

   Depositor

   Feng Zhang

   Insert

   SpsCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSaGuide

   Plasmid

   #64710

   Purpose

   Cloning and expression of Staphylococcus aureus guide RNA

   Depositor

   Kiran Musunuru

   Insert

   Staphylococcus aureus guide RNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEMPTY::sgRNA2

   Plasmid

   #165459

   Purpose

   Escherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteria

   Depositor

   Inigo Lasa

   Article

   Dorado-Morales et al mBio. 2021 Feb 23;12(1). pii: mBio.03094

   Insert

   sgRNA2
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   SP01

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSaGuide

   Plasmid

   #64710

   Purpose

   Cloning and expression of Staphylococcus aureus guide RNA

   Depositor

   Kiran Musunuru

   Insert

   Staphylococcus aureus guide RNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET22B_PcrA_2Bchimera

   Plasmid

   #102998

   Purpose

   Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA

   Depositor

   Martin Webb

   Insert

   PcrA_2Bchimera
   

   Expression

   Bacterial

   Mutation

   Bacilllus stearothermophilus PcrA in which the 2B…

   Promoter

   T7

   Available Since

   May 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSaCas9_GFP

   Plasmid

   #64709

   Purpose

   Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells

   Depositor

   Kiran Musunuru

   Insert

   SaCas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP and 3xFLAG-SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTH1-SaSrtA-Q

   Plasmid

   #64980

   Purpose

   Expresses the E108Q mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, E108Q

   Available Since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTH1-SaSrtA-A

   Plasmid

   #64979

   Purpose

   Expresses the E108A mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, E108A

   Available Since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTH1-SaSrtA-K

    Plasmid

    #64978

    Purpose

    Expresses the E105K mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET22B_PcrA_2Bchimera

    Plasmid

    #102998

    Purpose

    Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA

    Depositor

    Martin Webb

    Insert

    PcrA_2Bchimera
    

    Expression

    Bacterial

    Mutation

    Bacilllus stearothermophilus PcrA in which the 2B…

    Promoter

    T7

    Available Since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTH1-SaSrtA

    Plasmid

    #64973

    Purpose

    Expresses the wild type of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    T7 promoter

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTH1-SaSrtA-KA

    Plasmid

    #64981

    Purpose

    Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K, E108A

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTH1-SaSrtA-KQ

    Plasmid

    #64982

    Purpose

    Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K, E108Q

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSaCas9_GFP

    Plasmid

    #64709

    Purpose

    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells

    Depositor

    Kiran Musunuru

    Insert

    SaCas9-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP and 3xFLAG-SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRMC2

    Plasmid

    #68940

    Purpose

    tetracycline-inducible expression vector for Staphylococcus aureus

    Depositor

    Tim Foster

    Article

    Corrigan et al Plasmid. 2009 Mar;61(2):126-9. doi: 10.1

    Type

    Empty backbone

    Use

    Tet inducible e. coli/staphylococcus shuttle vect…

    Expression

    Bacterial

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIMAY*

    Plasmid

    #121441

    Purpose

    Allelic exchange plasmid with PheS* counter-selection for Staphylococcus aureus

    Depositor

    Angelika Grundling

    Article

    Schuster et al Microbiology. 2019 Apr 3. doi: 10.1099/m

    Type

    Empty backbone

    Use

    Escherichia coli/staphylococcus aureus shuttle ve…

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTH1-SaSrtA-Q

    Plasmid

    #64980

    Purpose

    Expresses the E108Q mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E108Q

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTH1-SaSrtA-A

    Plasmid

    #64979

    Purpose

    Expresses the E108A mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E108A

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTH1-SaSrtA-K

    Plasmid

    #64978

    Purpose

    Expresses the E105K mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge