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pXD80ENSF9-Pct3-(–)-Eldh2(cHis6)-Accs(ElA2)

Plasmid

#233868

Purpose

Expresses His-tagged Eggerthella lenta A2 (–)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh2

Tags

His

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN16-HcdhA(cHis8)BC-partial cluster(ElA2)

Plasmid

#225328

Purpose

Expresses Eggerthella lenta A2 Hcdh partial gene cluster (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Hcdh partial gene cluster

Tags

His8

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN20-Pct5-GPA_25810-(+)-Eldh1(GpDSM19378)

Plasmid

#225306

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh1

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN21-Pct5-GPA_25800-(+)-Eldh2(GpDSM19378)

Plasmid

#225307

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh2

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF25-Pct3-(–)-Eddh(nHis6)(G3C)

Plasmid

#233873

Purpose

Expresses His-tagged Gordonibacter pamelaeae 3C (–)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eddh

Tags

His

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF30-DadhABC(cHis8)RS-Accs(Cadh)(ElA2)

Plasmid

#233876

Purpose

Expresses Eggerthella lenta A2 Dadh with the accessory genes from the cadh gene cluster in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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SBC015910

Plasmid

#226287

Purpose

Expresses PhCFAT and PhIGS1 from trc promoter. Biosynthesis of isoeugenol from coniferyl alcohol.

Depositor

Rainer Breitling

Article

Chua et al Metab Eng Commun. 2024 May 15;18:e00237.

Inserts

Coniferyl alcohol acyltransferase
Isoeugenol synthase 1

Expression

Bacterial

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pIA036

Plasmid

#200425

Purpose

amyE::(Ppen-lacIΔ11-bsdronPA cm). PALM integration vector includes codon optimized dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.

Depositor

Rut Carballido-López

Article

Altinoglu et al Microbiol Spectr. 2024 Nov 4;12(12):e016

Insert

LacI

Tags

bsdronPA

Expression

Bacterial

Promoter

Ppen

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCMJ074

Plasmid

#215654

Purpose

pET28b derived T7 promoter with a 5’-UTR transcriptional fusion to clac143

Depositor

Brian Pfleger

Article

Jones et al Appl Microbiol Biotechnol. 2021 Feb;105(

Insert

Yellow fluorescent protein

Use

Synthetic Biology

Tags

10x histidine tag

Expression

Bacterial

Promoter

T7

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMAT_41BBICD

Plasmid

#197097

Purpose

This plasmid contains the coding sequence for the intracellular domain of 4-1BB. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

Depositor

Wendell Lim

Article

Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

Insert

This plasmid contains the coding sequence for the intracellular domain of 4-1BB.

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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