We narrowed to 2,341 results for: PUC19

1. AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor

   Plasmid

   #178087

   Purpose

   HDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   mScarlet-I mTOR signaling indicator (TOSI)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Human PGK1

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATP1A1_G2_Dual_sgRNA

   Plasmid

   #173201

   Purpose

   Coselection for ABE or NHEJ in human cells. Vector for tandem expression of ATP1A1 G2 sgRNA with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G2 sgRNA + user-specified sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Tandem U6 promoters

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pP2A_NLS_sfGFP

   Plasmid

   #63709

   Purpose

   Expressing P2A_NLS_sfGFP with homologous arm of Nanog.

   Depositor

   Stanley Qi

   Article

   Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

   Insert

   Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
   

   Use

   Just a donor dna with nanog homologous arm for kn…

   Tags

   sfGFP

   Available Since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJBL728

   Plasmid

   #140398

   Purpose

   Expresses CsoR-regulated 3WJdB RNA aptamer driven by T7 promoter with the csoO sequence 5BP downstream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-csoO-3WJdB-T
   

   Use

   Synthetic Biology

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJBL716

   Plasmid

   #140386

   Purpose

   Expresses MphR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mphO sequence 5BP downstream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-mphO-3WJdB-T
   

   Use

   Synthetic Biology

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-Solo-mStayGold-PXN

   Plasmid

   #227316

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   PXN Homology Arms flanking a mStayGold Tag (PXN Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. HBT-GCaMP6-HA

   Plasmid

   #107433

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   GCaMP6S
   

   Tags

   HA

   Expression

   Bacterial

   Available Since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAT15547_PEmax-SuperFi-Cas9

   Plasmid

   #184377

   Purpose

   Mammalian expression of SuperFi-Cas9 PEmax prime editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   PEmax-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   SV40 NLS and c-myc NLS

   Expression

   Mammalian

   Mutation

   H840A, R221K, N394K, Y1010D, Y1013D, Y1016D, V101…

   Promoter

   CMV

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Empty Target Control

   Plasmid

   #127667

   Purpose

   Encodes for the CRY2-HA and mCherry proteins expressed from the EF1a promoter

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   CRY2-HA-2A-mCherry
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-Solo-mScarlet-H2BC11

    Plasmid

    #207756

    Purpose

    Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pJB332

    Plasmid

    #115949

    Purpose

    ORF insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 TetR-Gly9-DDX6 (contains SapI)
    

    Use

    Synthetic Biology

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTW122

    Plasmid

    #115962

    Purpose

    ORF insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 5'-2xTetApt-mVenus-PEST
    

    Use

    Synthetic Biology

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. KTK_354

    Plasmid

    #180525

    Purpose

    KTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJBL724

    Plasmid

    #140394

    Purpose

    Expresses HucR-regulated 3WJdB RNA aptamer driven by T7 promoter with the hucO sequence 5BP downstream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-hucO-3WJdB-T
    

    Use

    Synthetic Biology

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3xFlag-HaloTag-EZH2 HDR - pDY053

    Plasmid

    #171108

    Purpose

    Homology directed repair plasmid for generating HaloTag-EZH2 cells

    Depositor

    Thomas Cech

    Article

    Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

    Insert

    EZH2 (EZH2 Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJBL726

    Plasmid

    #140396

    Purpose

    Expresses SmtB-regulated 3WJdB RNA aptamer driven by T7 promoter with the smtO sequence 5BP downstream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-smtO-3WJdB-T
    

    Use

    Synthetic Biology

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRAC_DARIC(2-3)_CD22-BBz_AAV6_Donor

    Plasmid

    #211906

    Purpose

    Vector for AAV6 donor production. Targeted CD22-BBz DARIC transgene integration to the TRAC locus via homology-directed repair.

    Depositor

    Yannick Doyon

    Article

    Levesque et al bioRxiv 2023.09.14.557485

    Insert

    DARIC-CD22-BBz
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHS1528

    Plasmid

    #205982

    Purpose

    U6-MWEE01000013 Cas5-HNH DR-BpiI-DR

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas5-HNH DR-BpiI-DR
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. sgRNA scaffold-spacer-human U6

    Plasmid

    #214697

    Purpose

    provide "sgRNA scaffold-spacer-human U6" fragment

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Insert

    sgRNA scaffold-spacer-human U6
    

    Use

    CRISPR

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPGP_scFv

    Plasmid

    #183732

    Purpose

    Expresses anti-lysozyme scFv antibody

    Depositor

    Jun Ishii

    Article

    Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

    Insert

    Anti-lysozyme scFv
    

    Tags

    His6-tag

    Expression

    Yeast

    Promoter

    GAP promoter

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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