We narrowed to 7,284 results for: Trac;

1. pBAD-GINKO1

   Plasmid

   #113111

   Purpose

   Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   GINKO1
   

   Use

   Tags

   6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPBbsr2-Lifeact-miRFP703

   Plasmid

   #178530

   Purpose

   Expresses Lifeact-miRFP703 in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   Lifeact-miRFP703
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)

   Plasmid

   #159853

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - mNeon(PATC)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #112924

   Purpose

   piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
   

   Use

   Piggybac transposon vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSK221

   Plasmid

   #129249

   Purpose

   reverse yTRAP, tetR-responsive Neon reporter

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Insert

   pADH1(tetO2)-mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCB004

   Plasmid

   #134147

   Purpose

   Bacterial expression of human Ska3

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   human Ska3 (SKA3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C58

   Plasmid

   #171428

   Purpose

   Expression of FKBP-(GGS)X6-HaloTag C58 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   FKBP-(GGS)X6-HaloTag C58
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE30 PTBP1 isoform1

   Plasmid

   #108591

   Purpose

   Recombinant His tagged protein production

   Depositor

   Chris Smith

   Article

   Wollerton et al RNA. 2001 Jun;7(6):819-32.

   Insert

   PTBP1 isoform 1 (PTBP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   isoform 1 (isoform c)

   Promoter

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pN-PITCh-3XHA

   Plasmid

   #127881

   Purpose

   Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HA tagging

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Insert

   GFP-Puro-3XHA (PPP2CA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBluescriptSKII+ U6-sgRNA(F+E) ACTB

    Plasmid

    #74705

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.TBG mCherry-LDTS-eLIR

    Plasmid

    #189002

    Purpose

    lipophagy-inducing adaptor protein, AAV

    Depositor

    Atsushi Hoshino

    Article

    Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

    Insert

    LDTS-eLIR, mCherry
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-EF1a-NucGFP

    Plasmid

    #184706

    Purpose

    Lentiviral transfer plasmid for expression of NucGFP

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    NucGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMM441

    Plasmid

    #61271

    Purpose

    Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.

    Depositor

    Timothy Lu

    Article

    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

    Insert

    Cas9, tracrRNA, crRNA
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPy-CAG-Cre::ERT2-IRES-BSD

    Plasmid

    #48760

    Purpose

    CAG promoter driving Cre-ERT2 fusion-IRES-Blasticidin resistance

    Depositor

    Austin Smith

    Article

    Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

    Insert

    Cre::ERT2
    

    Use

    Ppy

    Tags

    ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTCAV-FLEx(loxP)-FlpO

    Plasmid

    #67829

    Purpose

    Cre-dependent expression of FlpO from CAV2 virus

    Depositor

    Liqun Luo

    Article

    Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

    Insert

    FlpO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. FKBP_LD 0_TEV

    Plasmid

    #58877

    Purpose

    MESA protease chain with Rapamycin FKBP ectodomain and no linker domain

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPI138

    Plasmid

    #113229

    Purpose

    extrachromosomal expression vector, expression of LifeAct-GFP

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Insert

    LifeAct
    

    Use

    Dictyostelium discoideum expression vector, extra…

    Tags

    GFP

    Expression

    Mutation

    Promoter

    act15

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME-PLIN2-tdTOMATO

    Plasmid

    #172533

    Purpose

    PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

    Depositor

    Richard White

    Article

    Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

    Insert

    plin2-tdtomato
    

    Use

    Tags

    Expression

    Mutation

    S80R, A81S, E144D, I148F, and R415H mutations in …

    Promoter

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCB301TOR-CRISPR

    Plasmid

    #164797

    Purpose

    For gene editing in oomycetes

    Depositor

    Miaoying Tian

    Article

    Gumtow et al Mol Plant Microbe Interact. 2018 Mar;31(

    Inserts

    PsNLS-hSpCas9
    sgRNA expression cassette
    

    Use

    Oomycete expression

    Tags

    Expression

    Mutation

    Promoter

    HAM34 and RPL41

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-TRE3G-NLS-tdMCP-mCherry

    Plasmid

    #99909

    Purpose

    For the expression of NLS-tdMCP-mCherry

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Insert

    NLS-tdMCP-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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