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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 10,969 results for: Vars;

Showing: 1781 - 1800 of 10969 results

  1. SnFR_V.7.1.2

    Plasmid
    #182809
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Insert
    V.7.1.2-CholineSnFR
    Use
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    182809-plasmid-map-sequence-id-358174

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  4. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid
    #195316
    Purpose
    Dox inducible lentiviral dead Cas9
    Depositor
    Raj Chari
    Insert
    dead Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    D10A; H840A
    Promoter
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    195316-plasmid-map-sequence-id-385750

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  5. pAAV_Efla_FLEX_APEX2-eGFPf

    Plasmid
    #171939
    Purpose
    Expresses a membrane targeted (farnesylation) APEX2-eGFP fusion protein
    Depositor
    David Berson
    Insert
    APEX2-eGFPf
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171939-plasmid-map-sequence-id-338504

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  7. pAAV_Ef1a_FLEX_mAPEX2_P2A_mRuby2

    Plasmid
    #171938
    Purpose
    Expresses membrane targeted (palmitoylation) APEX2 and mRuby2 as separate proteins
    Depositor
    David Berson
    Insert
    Apex2, mRuby2
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    171938-plasmid-map-sequence-id-338493

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  8. pAAV_hsyn_FLEX_tau-APEX2

    Plasmid
    #171937
    Purpose
    Expresses APEX2 linked to tau for labeling microtubules
    Depositor
    David Berson
    Insert
    tau-APEX2
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hsyn
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    171937-plasmid-map-sequence-id-338490

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  9. pLS1/-11

    Plasmid
    #31492
    Depositor
    Kathleen Matthews
    Insert
    Lactose repressor with 11 amino acid C-terminal deletion (lacI E. coli variant)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 21, 2012
    Availability
    Academic Institutions and Nonprofits only

  10. KP.3

    Plasmid
    #233340
    Purpose
    expressing SARS-CoV-2 KP.3 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (KP.3) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    F456L, Q493E, V1104L on the basis of JN.1
    Promoter
    CMV
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    233340-plasmid-map-sequence-id-472420

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  11. pJK648

    Plasmid
    #71611
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Phe79>Trp mutant (TdPurE2-F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes phenylalanine-79 to tryptophan
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71611-plasmid-map-sequence-id-131688

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  12. NOVA1 eGFP

    Plasmid
    #61275
    Purpose
    GFP-tagged ORF clone of Homo sapiens neuro-oncological ventral antigen 1 (NOVA1), transcript variant 1
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    NOVA1 (NOVA1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    61275-plasmid-map-sequence-id-99586

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  13. XEC

    Plasmid
    #233341
    Purpose
    expressing SARS-CoV-2 XEC spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (XEC) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T22N, F59S on the basis of KP.3
    Promoter
    CMV
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    233341-plasmid-map-sequence-id-472417

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  14. KP.3.1.1

    Plasmid
    #233342
    Purpose
    expressing SARS-CoV-2 KP.3.1.1 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (KP.3.1.1) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S31del on the basis of KP.3
    Promoter
    CMV
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    233342-plasmid-map-sequence-id-472416

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  15. pcDNA3.3_SARS2_XBB.1.5

    Plasmid
    #196585
    Purpose
    expressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (XBB.1.5)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G252V, F486P on the basis of XBB
    Promoter
    CMV
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196585-plasmid-map-sequence-id-386779

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  16. pCas9CR5-NG

    Plasmid
    #120228
    Purpose
    pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120228-plasmid-map-sequence-id-233263

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  17. pcDNA3.3_SARS2_EG.5.1

    Plasmid
    #207641
    Purpose
    pseudovirus plasmid that expresses spike of EG.5.1
    Depositor
    David Nemazee
    Insert
    Spike (EG.5.1) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Q52H, F456L on the basis of XBB.1.5
    Promoter
    CMV
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    207641-plasmid-map-sequence-id-437731

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  18. pCas9CR4-NG

    Plasmid
    #120225
    Purpose
    pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120225-plasmid-map-sequence-id-233264

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  20. pCMV6-LacZ-BbsI

    Plasmid
    #114671
    Purpose
    For one-step cloning (with blue/ white screening) of an open reading frame of interest for constitutive gene expression
    Depositor
    Lynne Postovit
    Insert
    LacZ
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Lac promoter
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    114671-plasmid-map-sequence-id-224108

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Showing: 1781 - 1800 of 10969 results