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pLEGFP-Y705F-STAT3

Plasmid

#71445

Purpose

Retroviral expression of Y705F-STAT3. Please note that this plasmid contains Y705F-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Mutation

Y705F

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
6598-HisSUMO1GG

Plasmid

#13376

Depositor

Anne Dejean

Article

Seeler et al Mol Cell Biol. 2001 May . 21(10):3314-24

Insert

SUMO1 (SUMO1 Human)

Use

T7 in vitro translation

Tags

His

Expression

Mammalian

Mutation

mature form ending with GG

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only

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pRK5M-TACE

Plasmid

#31714

Depositor

Rik Derynck

Article

Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

Insert

TACE (ADAM17 Human)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
HA-Foxo1ADA (pCMV5)

Plasmid

#12143

Depositor

Domenico Accili

Article

Nakae et al J Clin Invest. 2001 Nov . 108(9):1359-67

Insert

Foxo1 ADA (Foxo1 Mouse)

Tags

HA and myc

Expression

Mammalian

Mutation

Phosphorylation-deficient, also referred to as co…

Promoter

CMV

Available Since

Aug. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sglacZ: pHR-hU6-CasMINI sgRNA_#2 (sgLacZ); EF1a-Puro-T2A-BFP- WPRE

Plasmid

#176272

Purpose

Expression of non-targeting sgRNA of CasMINI

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI non-targeting sgRNA (sgLacZ) and BFP

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MDM2 S166D S186D

Plasmid

#16236

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Expression

Mammalian

Mutation

Ser166 and Ser186 replaced by Asp

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pME phluorin2-gpi

Plasmid

#73793

Purpose

ratiometric pHluorin2 with a gpi linkage sequence in a middle entry clone

Depositor

David Raible

Article

Stawicki et al Dis Model Mech. 2014 Jul;7(7):847-56. do

Insert

ratiometric pHluorin2-gpi

Use

Middle entry clone

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pRetroSuper-shSrc

Plasmid

#26982

Depositor

Joan Massague

Article

Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

Insert

shRNA Src (SRC Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pRK5-HA GST RagD 77L

Plasmid

#19308

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Tags

GST and HA

Expression

Mammalian

Mutation

S77L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_Ado1.0

Plasmid

#153285

Purpose

Express the adenosine sensor GRAB_Ado1.0 in neurons

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Has Service

AAV1

Insert

Adenosine sensor GRAB_Ado1.0

Use

AAV

Promoter

hSyn

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_TNF-SBP-mCherry

Plasmid

#65279

Purpose

synchronize trafficking of TNF from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pJFRC150-20XUAS-IVS-Flp1::PEST

Plasmid

#32132

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

Flippase1::PEST

Tags

PEST

Expression

Insect

Mutation

Aspartic Acid at Amino Acid 5

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D3

Plasmid

#10912

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin D3 (CCND3 Human)

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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ACS1

Plasmid

#13746

Depositor

John Denu

Article

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

Acetyl CoA Synthetase 1 (Acss2 Mouse)

Tags

His

Expression

Bacterial

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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pAdTrack-PPARdelta

Plasmid

#16529

Depositor

Bert Vogelstein

Article

He et al Cell. 1999 Oct 29. 99(3):335-45.

Insert

PPAR delta (PPARD Human)

Use

Adenoviral

Tags

2x HA (YDVPDYAS)

Expression

Mammalian

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

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pCruzHA SIRT1 G261A

Plasmid

#10963

Depositor

Toren Finkel

Article

Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

Insert

SIRT1 (Sirt1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

G261A

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin

Plasmid

#134858

Purpose

Expresses EGFP-tagged early endosome marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Ras-related protein 5A

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET-BiFC

Plasmid

#87856

Purpose

pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Inserts

Encodes for MCS & C-term half of mVenus
Encodes for MCS & N-term half of mVenus

Tags

BiFC C-terminal fragment

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA2-CAND1

Plasmid

#20719

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)

Tags

HA2

Expression

Mammalian

Available Since

April 21, 2009

Availability

Academic Institutions and Nonprofits only
T7-Elongin C-pcDNA3

Plasmid

#19998

Depositor

William Kaelin

Article

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

Elongin C (ELOC Human)

Tags

T7-tag

Expression

Mammalian

Available Since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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