Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 4,673 results for: pcr

Clear keywords
Showing: 1781 - 1800 of 4673 results

  2. GPR84-Tango

    Plasmid
    #66377
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR84 (GPR84 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles

  3. YFP(1-158)-beta-5 in pcDNAI/Amp

    Plasmid
    #54470
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-5. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFP(1-158)/beta-5 (GNB5 Human, Aequorea victoria)
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    YFP (1-158) includes a substitution of Met for Gl…
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    54470-plasmid-map-sequence-id-114865

    Enlarge

  4. pATF4-donor

    Plasmid
    #113805
    Purpose
    CRISPR donor plasmid to tag human transcription factor ATF4 with GFP
    Depositor
    Kevin White
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATF4 homology arms flanking EGFP-IRES-Neo cassette (ATF4 Human)
    Use
    CRISPR
    Mutation
    rs140428747
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113805-plasmid-map-sequence-id-231035

    Enlarge

  5. pHyg-PCUP1-AID*(N)

    Plasmid
    #99529
    Purpose
    N-terminal AID* degron cassette with CUP1 promoter and HygR marker for S. cerevisiae
    Depositor
    Helle Ulrich
    Insert
    IAA17(71-117) (AXR3 Mustard Weed)
    Use
    Pcr template for yeast tagging cassette
    Promoter
    CUP1
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99529-plasmid-map-sequence-id-192013

    Enlarge

  7. pZC13 (pFA6a-TEV-6xGly-V5-HphMX)

    Plasmid
    #44085
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44085-plasmid-map-sequence-id-61696

    Enlarge

  9. pTF273 (pFA6a-PSc-6xGly-12xHis-HphMX)

    Plasmid
    #44075
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, 12xHis tag, ADH1 transcription term…
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44075-plasmid-map-sequence-id-61677

    Enlarge

  10. pTF269 (pFA6a-PSc-6xGly-12xHis-KanMX)

    Plasmid
    #44087
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, 12xHis tag, ADH1 transcription term…
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44087-plasmid-map-sequence-id-61673

    Enlarge

  12. MRGPRX1-Tango

    Plasmid
    #66439
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MRGPRX1 (MRGPRX1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  13. CYSLTR2-Tango

    Plasmid
    #66267
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CYSLTR2 (CYSLTR2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pJW1328

    Plasmid
    #69488
    Purpose
    sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegans
    Depositor
    Jordan Ward
    Insert
    sgRNA(F+E) targeting pha-1
    Expression
    Worm
    Promoter
    R07E5.16 U6 promoter
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69488-plasmid-map-sequence-id-125217

    Enlarge

  16. pBALU9

    Plasmid
    #69324
    Purpose
    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd GFP intron;use C-term after STOP
    Depositor
    Oliver Hobert
    Publication
    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/
    Insert
    gSL2-NLS-GFPint-FgF
    Expression
    Bacterial
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. Cer(1-158)-gamma-5 in pcDNAI/Amp

    Plasmid
    #55625
    Purpose
    An amino-terminal mCerulean fragment was fused to Ggamma-5. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cer(1-158)-gamma-5 (GNG5 Human, Aequorea victoria)
    Tags
    Cer(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-5 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55625-plasmid-map-sequence-id-115180

    Enlarge

  20. CFP(159-238)-beta-5 in pcDNAI/Amp

    Plasmid
    #55763
    Purpose
    A carboxyl-terminal CFP fragment was fused to Gbeta-5. When co-expressed with an amino terminal CFP or YFP fragment fused to a Ggamma subunit or RGS7, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CFP(159-238)-beta-5 (GNB5 Human, Aequorea victoria)
    Tags
    CFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    CFP(159-238) includes a substitution of His for A…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    55763-plasmid-map-sequence-id-114867

    Enlarge

Showing: 1781 - 1800 of 4673 results