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KHLH31 pEGFP-N2 (1089)

Plasmid

#62032

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

KHLH31

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pFAB806

Plasmid

#47849

Purpose

BIOFAB reporter plasmid for measuring lambda tR2 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

lambda tR2 terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO4

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB805

Plasmid

#47850

Purpose

BIOFAB reporter plasmid for measuring tonB termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

tonB terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO5

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB816

Plasmid

#47851

Purpose

BIOFAB reporter plasmid for measuring ilvGEDA termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

ilvGEDA

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO6

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB807

Plasmid

#47852

Purpose

BIOFAB reporter plasmid for measuring RNAI termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

RNAI terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO7

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB820

Plasmid

#47855

Purpose

BIOFAB reporter plasmid for measuring tetAC termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

tetAC terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO10

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB1910

Plasmid

#47859

Purpose

BIOFAB reporter plasmid for measuring rpoC termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

rpoC terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO14

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB804

Plasmid

#47848

Purpose

BIOFAB reporter plasmid for measuring T3 early termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

T3 early terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO3

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3905

Plasmid

#47808

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pZT-AAVS1-R1

Plasmid

#52638

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Article

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Right AAVS1 TALEN

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2014

Availability

Academic Institutions and Nonprofits only

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pZT-AAVS1-L1

Plasmid

#52637

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Article

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Left AAVS1 TALEN

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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pOTTC407 - pAAV EF1a eGFP

Plasmid

#60058

Purpose

An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

enhanced Green Fluorescent Protein

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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NSUN6

Plasmid

#188060

Purpose

Bacterial expression plasmid for human NSUN6; for activity testing

Depositor

Tanja Schirmeister

Article

Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

Insert

NSUN6 (NSUN6 Human)

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pIVT_Dn29-dCas9-P2A-GFP

Plasmid

#247165

Purpose

In vitro transcription of human codon optimized fusion of Dn29-dCas9-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Dn29-dCas9-P2A-GFP

Use

In vitro transcription

Tags

SV40 NLS

Promoter

mutated T7

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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TNPO1-shRNA

Plasmid

#86082

Purpose

A lentiviral construct for building stable cell lines expressing Transportin-1 shRNA via puromycin selection.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Transportin-1 (TNPO1 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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NSUN2

Plasmid

#188059

Purpose

Bacterial expression plasmid for human NSUN2; for activity testing

Depositor

Tanja Schirmeister

Article

Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

Insert

NSUN2 (NSUN2 Human)

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pNHT7::RPL15::sGluc

Plasmid

#186769

Purpose

An mRNA production vector encoding planarian codon-optimized gaussia luciferase (sGluc) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Gaussia luciferase

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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p35S_GFP-GUS

Plasmid

#167122

Purpose

Plant binary expression vector containing mGFP5-GUS fusion reporter under CaMV 35S promoter.

Depositor

Arturo Marí-Ordóñez, Olivier Voinnet

Article

Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

Insert

GFP-GUS

Tags

mGFP5, GUS

Expression

Plant

Promoter

CaMV 35S

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAF112

Plasmid

#135920

Purpose

All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.

Depositor

Andrew S. Flies

Article

Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

Type

Empty backbone

Use

Luciferase; Transposon

Expression

Mammalian

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab1a S25N

Plasmid

#46777

Purpose

expression of S25N Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a S25N mutant (RAB1A Human)

Tags

myc

Expression

Mammalian

Mutation

S25N (dominant negative)

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only