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pEAHISMRSA0482

Plasmid

#96968

Purpose

expression of methicillin-resistant Staphylococcus aureus orf 0482

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

MRSA ORF0482

Tags

N-ter TEV protease cleavable 6His

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSA2028

Plasmid

#97001

Purpose

Expressing MRSA Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase

Tags

N-ter TEV protease cleavable 6His

Mutation

none

Promoter

T7

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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cleaveBADTrp

Plasmid

#242866

Purpose

Expression of cleavable Trp1 (extracellular domain)

Depositor

Ferenc Scheeren

Article

van der Plas – van Duijn et al Commun Biol 8, 1561 (2025).

Insert

ECD Trp-1-WT fused to cleavable TMD (TYRP1 Human)

Use

Lentiviral

Tags

Cleavable CAR TMD

Expression

Mammalian

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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Trp1-WT-display

Plasmid

#242867

Purpose

Expression of ECD of WT- Trp1 fused to display TMD.

Depositor

Ferenc Scheeren

Article

van der Plas – van Duijn et al Commun Biol 8, 1561 (2025).

Insert

ECD Trp-1-WT fused to display TMD (TYRP1 Human)

Use

Lentiviral

Tags

Display domain

Expression

Mammalian

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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Trp1-Ala-display

Plasmid

#242868

Purpose

Expression of ECD of Trp-1-ala fused to display TMD.

Depositor

Ferenc Scheeren

Article

van der Plas – van Duijn et al Commun Biol 8, 1561 (2025).

Insert

Trp-1-ala (TYRP1 Human)

Use

Lentiviral

Tags

Display domain

Expression

Mammalian

Mutation

First amino acid after signal peptide mutated fro…

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-ARID1A-deltaIDR2-FLAG

Plasmid

#242215

Purpose

Expression of human ARID1A ∆IDR2 (deletion of a.a. 1170-1610) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

ARID1A (ARID1A Human)

Use

Piggybac

Tags

FLAG

Expression

Mammalian

Mutation

Deleted IDR2 from ARID1A (deletion of a.a. 1170-1…

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-CTNNB1-delta1-3-HA

Plasmid

#242219

Purpose

Expression of human β-catenin ∆arm 1-3 (deletion of a.a. 134-266) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Deleted arm 1-3 from β-catenin (deletion of a.a. …

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-CTNNB1-Armadillo-HA

Plasmid

#242224

Purpose

Expression of human β-catenin Armadillo repeats (deletion of a.a. 2-133 and a.a. 665-781) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Deleted N terminal and C terminal IDRs from β-cat…

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-CTNNB1-deltaC-HA

Plasmid

#242223

Purpose

Expression of human β-catenin ∆C terminal IDR (deletion of a.a. 665-781) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Deleted C terminal IDR from β-catenin (deletion o…

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-ARID1A-deltaIDR1ARID-FLAG

Plasmid

#242211

Purpose

Expression of human ARID1A ∆IDR1 and ∆ARID (deletion of a.a. 2-1169) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

ARID1A (ARID1A Human)

Use

Piggybac

Tags

FLAG

Expression

Mammalian

Mutation

Deleted IDR1 and ARID from ARID1A (deletion of a.…

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-CTNNB1-delta4-6-HA

Plasmid

#242220

Purpose

Expression of human β-catenin ∆arm 4-6 (deletion of a.a. 268-394) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Deleted arm 4-6 from β-catenin (deletion of a.a. …

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-CTNNB1-delta10-12-HA

Plasmid

#242222

Purpose

Expression of human β-catenin ∆arm 10-12 (deletion of a.a. 522-664) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Deleted arm 10-12 from β-catenin (deletion of a.a…

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1-GST-Armadillo

Plasmid

#242225

Purpose

Expression of GST-tagged human β-catenin Armadillo repeats without IDRs (deletion of a.a. 2-133 and 672-781) in E. coli

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Tags

GST

Expression

Bacterial

Mutation

Deleted N terminal and C terminal IDRs from β-cat…

Promoter

tac

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pMAL-c4X-MBP-ARID1A-IDR2

Plasmid

#242216

Purpose

Expression of MBP-tagged human ARID1A IDR2 (a.a. 1170-1610) in E. coli

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

ARID1A (ARID1A Human)

Tags

MBP

Expression

Bacterial

Mutation

ARID1A IDR2 (a.a. 1170-1610)

Promoter

tac

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-CTNNB1-deltaN-HA

Plasmid

#242218

Purpose

Expression of human β-catenin ∆N terminal IDR (deletion of a.a. 2-133) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Deleted N terminal IDR from β-catenin (deletion o…

Promoter

CAG

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1-GST-Armadillo-delta4-6

Plasmid

#242226

Purpose

Expression of GST-tagged human β-catenin Armadillo repeats ∆4-6 without IDRs (deletion of a.a. 2-133, 268-394, and 672-781) in E. coli

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Tags

GST

Expression

Bacterial

Mutation

Deleted N terminal IDR, arm 4-6, and C terminal I…

Promoter

tac

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-CTNNB1-delta7-9-HA

Plasmid

#242221

Purpose

Expression of human β-catenin ∆arm 7-9 (deletion of a.a. 395-520) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Deleted arm 7-9 from β-catenin (deletion of a.a. …

Promoter

CAG

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEAHISSOarD1

Plasmid

#97067

Purpose

Expressing Sulfolobus solfataricus orf Sso Ard1 (N-terminal acetylase) protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus Sso Ard1 protein

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSIRV131

Plasmid

#97053

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 131 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 131

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSIRV418/gp25

Plasmid

#97052

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 418/gp25 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 418/gp25

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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