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  1. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...reporter (ERK KTR-mRuby2). The ERK KTR is a target for MEK1 phosphorylation, much like endogenous ERK, and...inhibitors to alter Raf1-MEK-ERK pathway signaling by using psMEK1 and an ERK kinase translocation reporter...phosphorylates the ERK KTR and red fluorescence localizes to the cytoplasm. When MEK1 and ERK are inactive,...localization of ERK KTR-mRuby2 determined. If the inhibitor targeted MEK1 or its downstream target ERK, the KTR...inactive, the ERK KTR isn’t phosphorylated and red fluorescence can be found in the nucleus. For testing kinase...kinase inhibitors, psMEK1 and ERK KTR-mRuby2 expressing cells were incubated with an inhibitor of choice...localized to the cytoplasm. This all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing...

  2. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...OptoNB-SOS activated Ras/Erk signaling by using a fluorescent reporter for Erk kinase translocation. These...proof-of-principle, the team created an OptoNB to control the Ras/Erk signaling pathway, which is often over-activated ...initiates the extracellular signaling-regulated kinase (ERK) signaling cascade. This signaling is a lot like ...harnessed to regulate signaling pathways such as the Ras/Erk pathway. What’s next for OptoNBs? While OptoNBs illuminate...

  3. MAPK Plasmids

    Type
    Collection
    ...MAPK3 ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK MAPK4 ERK3, Erk4...organized by gene) Gene Alternate Names MAPK1 ERK, ERK2, ERT1, MAPK2, P42MAPK, ...known as extracellular signal-regulated kinases (ERKs), are serine/threonine-specific protein kinases....Erk4, PRKM4, p63MAPK MAPK6 ERK3, HsT17250, PRKM6, p97MAPK MAPK7 BMK1, ERK4, ERK5, PRKM7 MAPK8 JNK, JNK-46... PRKM11, SAPK2, SAPK2B, p38-2, p38Beta MAPK12 ERK3, ERK6, P38GAMMA, PRKM12, SAPK-3, SAPK3 MAPK13 RP1-179N16.4... PRKM15, RK, SAPK2A, p38, p38ALPHA MAPK15 ERK7, ERK8 *Image is licensed under the GFDL. Source: Wikipedia...

  5. mTOR Pathway

    Type
    Collection
    ...mTORC1 is activated downstream of PI3K/Akt and MAPK/ERK, both of which are commonly dysregulated in cancer...

  8. Ras Pathway

    Type
    Collection
    ... protein ERBB2 Erb-b2 receptor tyrosine kinase 2 ERK MAPK1 MAPK3 Also known as MAPK; Mitogen-activated...

  10. Video Library

    Type
    Protocol
    ...students considering their future careers. Eric J. Perkins, PhD In this installment, we sit down with Senior...Senior Scientific Project Leader Eric Perkins to discuss the various positions he has held in his career...

  11. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Vladislav Verkhusha 31949 pPAmCherry-b-actin Actin Filaments beta-actin PAmCherry Vladislav Verkhusha 79990...Vladislav Verkhusha 79991 pmiRFP703-Tubulin Microtubules alpha-tubulin miRFP703 Vladislav Verkhusha 79993 ...miRFP703 Vladislav Verkhusha 79994 pEB3-miRFP703 Microtubules EB3 miRFP703 Vladislav Verkhusha 79996 pVimentin-miRFP703...Vladislav Verkhusha 79999 pZyxin-miRFP703 Focal Adhesions Zyxin miRFP703 Vladislav Verkhusha 55023 mCherry-Cx43...pMito-miRFP703 Mitochondria COX8A miRFP703 Vladislav Verkhusha 36208 pmTurquoise2-Mito Mitochondria COX8A(1-29...pH2B-miRFP703 Chromatin H2B miRFP703 Vladislav Verkhusha 17650 GFP-HP1gamma Chromatin HP1 gamma GFP Tom...Filaments (vimentin) Vimentin miRFP703 Vladislav Verkhusha 26737 GFP-UtrCH Actin Filaments Utrophin (aa# ...

  12. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...you’ll pretty quickly notice the name Vladislav Verkhusha popping up again and again, and for good reason... those tools are great for many applications, Verkhusha and his lab at Albert Einstein College of Medicine... mammalian tissues are nearly transparent. As Verkhusha explained it to me, the transparency window of...the transparency window of mammalian tissues,” Verkhusha said. “We developed near-infrared fluorescent ...deeper.” Adding to the toolbox In the last year, Verkhusha added two far-red light photoactivatable (PA) ...background.” Just last month in Scientific Reports, Verkhusha’s team reported another advance in the application...is because the light becomes very scattered,” Verkhusha explained. Ultrasound wavelengths, on the other...

  13. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...resources References Shemetov, A.A., Oliinyk, O.S. & Verkhusha, V.V. How to Increase Brightness of Near-Infrared...4557 (2017). Shcherbakova, D.M., Baloban, M. & Verkhusha, V.V. Near-infrared fluorescent proteins engineered...Shcherbakova, D.M., Stepanenko, O.V., Turoverov, K.K. & Verkhusha, V.V. Near-Infrared Fluorescent Proteins: Multiplexing...Shemetov, A.A., Pletnev, S., Shcherbakova, D.M. & Verkhusha, V.V. Smallest near-infrared fluorescent protein...Nat Methods 11, 641-644 (2014). Filonov, G.S. & Verkhusha, V.V. A near-infrared BiFC reporter for in vivo...Shcherbakova, D.M., Cox Cammer, N., Huisman, T.M., Verkhusha, V.V. & Hodgson, L. Direct multiplex imaging and...Nat Commun 5, 3626 (2014). Shcherbakova, D.M. & Verkhusha, V.V. Near-infrared fluorescent proteins for multicolor...

  14. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...they are also toxic,” Merkx explained. New tools were needed to measure them. Merkx says, while in principle...? That is not known.” Transition Metals Maarten Merkx of Technische Universiteit Eindhoven has similar...FRET sensor with a large dynamic range,” he says. Merkx recently applied this “trick” to the development...different molecules like zinc and calcium. Ultimately, Merkx said he hopes others will find his tools useful,...depositing at Addgene, people will start using them,” Merkx said. “For us, it is one of the main measures of...

  16. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ... Addgene’s Director of Product Management Eric Perkins, who has been in many roles in Addgene since he... really get me where I haven’t gotten myself.” Perkins noted that there is a more emphasis today about...those doors to be opened.” Addgenie Eric Perkins presented a poster a few years ago at a CRISPR ...

  17. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...Patrick Hsu’s lab at UC Berkeley. She will be starting her PhD at UC Berkeley in Microbiology in fall ...Research Specialist in Patrick Hsu's lab at UC Berkeley. Most of the scientists I’ve met began doing research...

  18. Fluorescent Protein Guide: Biosensors

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    Collection
    ...Sensor. PLoS One. 2013 Dec 2;8(12):e82009. Maarten Merkx Potassium Green fluorescence indicator of K+ for...homeostasis. Nat Methods. 2009 Oct;6(10):737-40. Maarten Merkx Zinc eZinCh-2 Zn2+ FRET sensor, cytosolic or ER-...ACS Chem Biol. 2015 Sep 18;10(9):2126-34. Maarten Merkx Zinc Cytosolic Cyan-Yellow Zinc FRET sensors ZapCV2...Neurosci. 2020 Nov 4;11(21):3523-3531. Vladislav Verkhusha Return to Top Browse All Looking for a sensor ...

  19. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ... BioBrick BB-2 (RFC 12) Back to Top BglBrick – Berkeley Standard (RFC 21) Prefix: GAATTC ATG AGATCT Prefix...BamHI, XhoI For more info, visit iGEM: BglBrick – Berkeley Standard (RFC 21) Back to Top Silver Standard ...

  20. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...with scientists at the University of California, Berkeley, the Janelia Research Campus also developed a ...by endothelial transcytosis (Foust et al., 2009; Merkel et al., 2017), and these two modes of transport...PMID: 19250335. PubMed Central PMCID: PMC2698947. Merkel, S.F., Andrews, A.M., Lutton, E.M., Mu, D., Hudry...
Showing: 1 - 20 of 79 results