...reporter (ERK KTR-mRuby2). The ERK KTR is a target for MEK1 phosphorylation, much like endogenous ERK, and...inhibitors to alter Raf1-MEK-ERK pathway signaling by using psMEK1 and an ERK kinase translocation reporter...phosphorylates the ERK KTR and red fluorescence localizes to the cytoplasm. When MEK1 and ERK are inactive,...localization of ERK KTR-mRuby2 determined. If the inhibitor targeted MEK1 or its downstream target ERK, the KTR...inactive, the ERK KTR isn’t phosphorylated and red fluorescence can be found in the nucleus. For testing kinase...kinase inhibitors, psMEK1 and ERK KTR-mRuby2 expressing cells were incubated with an inhibitor of choice...localized to the cytoplasm. This all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing...

...OptoNB-SOS activated Ras/Erk signaling by using a fluorescent reporter for Erk kinase translocation. These...proof-of-principle, the team created an OptoNB to control the Ras/Erk signaling pathway, which is often over-activated ...initiates the extracellular signaling-regulated kinase (ERK) signaling cascade. This signaling is a lot like ...harnessed to regulate signaling pathways such as the Ras/Erk pathway. What’s next for OptoNBs? While OptoNBs illuminate...

...looking for a ready-to-use KTRs for p38, JNK, and ERK, or a backbone to use for custom KTR design, plasmids...

...Xin’s work with photoswitchable components of the ERK signaling pathway in our recent blog post. Fatima...

...lead to increased activity of the AKT, mTor, and ERK pathways so the lab will be studying the effects ...

...synthesized the activated form of human mitogen-activated ERK activating kinase 1 (MEK1) with either one or two...

...you’ll pretty quickly notice the name Vladislav Verkhusha popping up again and again, and for good reason... those tools are great for many applications, Verkhusha and his lab at Albert Einstein College of Medicine... mammalian tissues are nearly transparent. As Verkhusha explained it to me, the transparency window of...the transparency window of mammalian tissues,” Verkhusha said. “We developed near-infrared fluorescent ...deeper.” Adding to the toolbox In the last year, Verkhusha added two far-red light photoactivatable (PA) ...background.” Just last month in Scientific Reports, Verkhusha’s team reported another advance in the application...is because the light becomes very scattered,” Verkhusha explained. Ultrasound wavelengths, on the other...

...resources References Shemetov, A.A., Oliinyk, O.S. & Verkhusha, V.V. How to Increase Brightness of Near-Infrared...4557 (2017). Shcherbakova, D.M., Baloban, M. & Verkhusha, V.V. Near-infrared fluorescent proteins engineered...Shcherbakova, D.M., Stepanenko, O.V., Turoverov, K.K. & Verkhusha, V.V. Near-Infrared Fluorescent Proteins: Multiplexing...Shemetov, A.A., Pletnev, S., Shcherbakova, D.M. & Verkhusha, V.V. Smallest near-infrared fluorescent protein...Nat Methods 11, 641-644 (2014). Filonov, G.S. & Verkhusha, V.V. A near-infrared BiFC reporter for in vivo...Shcherbakova, D.M., Cox Cammer, N., Huisman, T.M., Verkhusha, V.V. & Hodgson, L. Direct multiplex imaging and...Nat Commun 5, 3626 (2014). Shcherbakova, D.M. & Verkhusha, V.V. Near-infrared fluorescent proteins for multicolor...

...they are also toxic,” Merkx explained. New tools were needed to measure them. Merkx says, while in principle...? That is not known.” Transition Metals Maarten Merkx of Technische Universiteit Eindhoven has similar...FRET sensor with a large dynamic range,” he says. Merkx recently applied this “trick” to the development...different molecules like zinc and calcium. Ultimately, Merkx said he hopes others will find his tools useful,...depositing at Addgene, people will start using them,” Merkx said. “For us, it is one of the main measures of...

...Interactions. Filonov GS, Verkhusha VV. Chem Biol. 2013 Vladislav Verkhusha iRFP GAF domains from miRFP709...Shcherbakova et al. Nat Commun. 2016 Vladislav Verkhusha Multiple FAST Fluorescence-activating and absorption...

... Addgene’s Director of Product Management Eric Perkins, who has been in many roles in Addgene since he... really get me where I haven’t gotten myself.” Perkins noted that there is a more emphasis today about...those doors to be opened.” Addgenie Eric Perkins presented a poster a few years ago at a CRISPR ...

...Patrick Hsu’s lab at UC Berkeley. She will be starting her PhD at UC Berkeley in Microbiology in fall ...Research Specialist in Patrick Hsu's lab at UC Berkeley. Most of the scientists I’ve met began doing research...

...with scientists at the University of California, Berkeley, the Janelia Research Campus also developed a ...by endothelial transcytosis (Foust et al., 2009; Merkel et al., 2017), and these two modes of transport...PMID: 19250335. PubMed Central PMCID: PMC2698947. Merkel, S.F., Andrews, A.M., Lutton, E.M., Mu, D., Hudry...

...overexpressing at the time of use. Another great viral perk for delivery is targetability. As mentioned prior...EBC20150001 Leopold, A. V., Shcherbakova D. M., Verkhusha, V. V., Fluorescent Biosensors for Neurotransmission...

...Martin, and Berkley Gryder. Addgene depositors or not, your time is valuable ... Eric J. Perkins...

... Harvard Medical School, and the Doudna Lab at Berkeley set out to decrease the off target effects of ...development of HypaCas9 Researchers in the Doudna Lab at Berkeley recently added their own hyper accurate Cas9 variant...

...that matures quickly and the other slowly, see Verkhusha et al.), one benefit of the common FP timers is...19889833. PubMed Central PMCID: PMC2801723. 3. Verkhusha, Vladislav V., et al. "An enhanced mutant of red...

...Fluorescent Protein Guide Expression Vectors from the Berkeley QB3 MacroLab Eric thanks his wife, Annette Sievers... Eric J. Perkins...

..., a researcher at the University of California Berkeley. It’s been half a year since the march for science...evolutionary arms race at University of California, Berkeley. She is a scientist with a passion for photosynthetic...

...Addgenie Eric Perkins attended the recent Keystone Meeting "Precision Genome Engineering and Synthetic...