We narrowed to 7,128 results for: Trac;

1. pMito-dCherry-FRB

   Plasmid

   #186573

   Purpose

   Expression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cells

   Depositor

   Stephen Royle

   Article

   Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

   Insert

   Dark MitoTrap
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mutation of "K70N" to render mCherry no…

   Promoter

   CMV

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFJ1149 - intron 3 smu-2 PATCs

   Plasmid

   #161516

   Purpose

   PATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron 3 from smu-2
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113344

   Purpose

   Expresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   VP64-dCas9-p300core-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEM253 - sgRNA mosTI hygroR

   Plasmid

   #159827

   Purpose

   Single copy or array insertion by MosTI using split hygroR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI hygroR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFvL118

   Plasmid

   #64752

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a - SodA

   Plasmid

   #89612

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Superoxide dismutase A
   

   Use

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-R1R2_EF1adCas9p300_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113342

   Purpose

   Expresses dCas9-p300 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   dCas9-p300core-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTW081

   Plasmid

   #64755

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA epitope tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.GFAP.(cyto).cpSFGFP.HaloTag

   Plasmid

   #209662

   Purpose

   Non-responsive control

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   cpSFGFP.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   GFAP

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. NpT7-5-Erk2

    Plasmid

    #39230

    Depositor

    Melanie Cobb

    Article

    Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

    Insert

    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pKLV2-EF1a-NucGFP

    Plasmid

    #184696

    Purpose

    Lentiviral transfer plasmid for expression of NucGFP

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    NucGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2 mouse CV2 PC

    Plasmid

    #25776

    Depositor

    Edward De Robertis

    Article

    Coffinier et al Mech Dev. 2002 Dec . 119 Suppl 1():S179-

    Insert

    cross veinless 2 (Bmper Mouse)
    

    Use

    Tags

    PC

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. cacnb1b (rat) in pMT2 vector

    Plasmid

    #107423

    Purpose

    Expresses Calcium channel beta1b auxillary subunit in mammalian cells.

    Depositor

    Annette Dolphin

    Article

    Page et al J Neurosci. 1997 Feb 15;17(4):1330-8.

    Insert

    calcium channel beta1b auxillary subunit
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ad MLP

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Myc tag S16-A PTB

    Plasmid

    #23025

    Depositor

    Douglas Black

    Article

    Xie et al Proc Natl Acad Sci U S A. 2003 Jul 22. 1

    Insert

    PTB1 (PTBP1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Changed Serine 16 to Alanine

    Promoter

    Available since

    June 4, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pGAN213

    Plasmid

    #129270

    Purpose

    Control for measuring background levels of yTRAP reporter protein

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    min pCYC1

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS416gT-GalL-Cas9

    Plasmid

    #79903

    Purpose

    pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression

    Depositor

    Ronald Davis

    Article

    Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw

    Inserts

    Cas9
    guide RNA (gRNA)
    Tetracycline Repressor
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPM1 promoter, GalL, and RPR1 promoter with TetO …

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCFJ2370 - intron GG3 - 250bp

    Plasmid

    #159879

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    intron GG3 - 250bp
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSEM270 - MCS mosTI hygroR target

    Plasmid

    #159826

    Purpose

    Single copy or array insertion by MosTI using split hygroR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    MCS mosTI hygroR target
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. SNV-gfp-IRES-MCS

    Plasmid

    #53122

    Purpose

    Retrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cells

    Depositor

    Jeanette Hyer

    Article

    Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1

    Type

    Empty backbone

    Use

    Retroviral; For expression in chicken cells

    Tags

    EGFP-IRES

    Expression

    Mutation

    Promoter

    SNV (spleen necrosis virus)

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-EF1a-GFP ArgSen

    Plasmid

    #184695

    Purpose

    Lentiviral transfer plasmid for expression of GFP ArgSen

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    GFP ArgSen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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