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SIN40C.SFFV.dCas9-KRAB.P2A.mNeon

Plasmid

#170482

Purpose

Lentiviral dSpCas9-KRAB (CRISPRi) delivery, mNeon coexpression

Depositor

Dirk Heckl

Article

Ng et al iScience. 2023 Sep 7;26(10):107844. doi:

Inserts

dCas9-KRAB
SFFV
P2A-mNeonGreen

Use

Lentiviral

Tags

HA

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pSKC12 - DelQ

Plasmid

#110493

Purpose

Monitor the expression of Luciferase driven by a mutated NRAS 5'-UTR.

Depositor

Shankar Balasubramanian

Article

Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

Insert

NRAS 5'- DelQ (bp 30-254) (NRAS Human)

Use

Luciferase

Tags

Firefly luciferase

Mutation

Deletion of the first 29 base pairs of the NRAS 5…

Promoter

T7

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-BLM DM

Plasmid

#80071

Purpose

Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

Depositor

Nathan Ellis

Article

Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

Insert

BLM (BLM Human)

Tags

GFP-BLM

Expression

Mammalian

Mutation

K317R (AAA>AGA) and K331R (AAA>AGA)

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO5d.SFFV.eGFP-miR30n

Plasmid

#90333

Purpose

miR30 based shMir knockdown

Depositor

Dirk Heckl

Article

Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

Inserts

eGFP
na
SFFV
miR30n

Use

Lentiviral

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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beta catenin binding mutant of alpha catenin sensor

Plasmid

#71711

Purpose

This form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctions

Depositor

Deborah Leckband

Article

Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

Insert

alpha-catenin Δβ-cat mutant FRET Sensor

Tags

ECFP and YPET

Expression

Mammalian

Mutation

Deleted amino acid residues 48- 163

Promoter

CMV

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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VBS mutant of alpha catenin sensor

Plasmid

#71710

Purpose

This form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.

Depositor

Deborah Leckband

Article

Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

Insert

alpha-catenin ΔVBS FRET Sensor

Tags

ECFP and YPET

Expression

Mammalian

Mutation

Deleted amino acid residues 316-405 in α -catenin…

Promoter

CMV

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO5d.SFFV.dTomato-miR30n

Plasmid

#90334

Purpose

miR30 based shMir knockdown

Depositor

Dirk Heckl

Article

Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

Inserts

dTomato
na
SFFV
miR30n

Use

Lentiviral

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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gp64-mVEGF-C-HisTag-pENTR

Plasmid

#64065

Purpose

Plasmid encodes mature form of murine VEGF-C preceded by baculoviral gp64 secretion signal; entry vector to Gateway cloning system.

Depositor

Joanna Bereta

Article

Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

Insert

vascular endothelial growth factor C (Vegfc Mouse)

Tags

His-tag

Expression

Insect

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pET28-MRPP3-His

Plasmid

#67866

Purpose

bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag

Depositor

Walter Rossmanith

Article

Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

Insert

MRPP3 (KIAA0391 Human)

Tags

His

Expression

Bacterial

Mutation

Amino acid 46 of recombinant MRPP3 is preceded by…

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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Delta DED FLIP-L D/A

Plasmid

#13791

Depositor

Guy Salvesen

Article

Boatright et al Biochem J. 2004 Sep 1. 382(Pt 2):651-7.

Insert

DELTA DED FLIP LONG (CFLAR Human)

Tags

8 x His tag

Expression

Bacterial

Mutation

Delta DED removes first 186 residues Asp to Ala m…

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

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pET28-MRPP3-myc-His

Plasmid

#67864

Purpose

bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tag

Depositor

Walter Rossmanith

Article

Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

Insert

MRPP3 (KIAA0391 Human)

Tags

His and myc

Expression

Bacterial

Mutation

Amino acid 46 of recombinant MRPP3 is preceded by…

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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HBM-mVEGF-C-HisTag-pENTR

Plasmid

#64066

Purpose

Plasmid encodes mature form of murine VEGF-C preceded by honey bee melittin secretion signal; entry vector to Gateway cloning system.

Depositor

Joanna Bereta

Article

Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

Insert

vascular endothelial growth factor C (Vegfc Mouse)

Tags

His-tag

Expression

Insect

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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USPQ-HTT-TALEN1

Plasmid

#92243

Purpose

TALEN targeting upstream of HTT gene (KKR), forms obligate heterodimer with USPQ-HTT-TALEN2

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

TALEN

Use

TALEN

Promoter

CMV

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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USPQ-HTT-TALEN2

Plasmid

#92244

Purpose

TALEN targeting upstream of HTT gene (ELD), forms obligate heterodimer with USPQ-HTT-TALEN1

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

TALEN

Use

TALEN

Promoter

CMV

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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His-MBP-NRBF2 (1-159)

Plasmid

#99331

Purpose

Mammalian expression of the monomeric form of NRBF2 that binds with the PI3K C1 complex

Depositor

James Hurley

Article

Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

Insert

Nuclear receptor-binding factor 2 (NRBF2 Human)

Tags

6xHis-MBP-TEV

Expression

Bacterial

Mutation

AA1-159 are present

Promoter

T7

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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CRY-GalVP16l (B694)

Plasmid

#92030

Purpose

expresses CRY2 (Full length, mutant NLS) fused to Gal4DNA (residues 1-147) fused to VP16 activation domain (long form)

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Tags

GalBD-VP16

Expression

Mammalian

Mutation

NLS sequence of CRY2 mutated

Promoter

CMV

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pJTB115

Plasmid

#248909

Purpose

Expresses yeast Smk1 and Ssp2 fused to glutathione-S-transferase in bacteria

Depositor

Edward Winter

Article

Tio et al Mol Cell Biol. 2017 May 2;37(10):e00607-

Inserts

SMK1 Mitogen-activated Protein Kinase (SMK1 Budding Yeast, Sequence is from the SK1 isolate of S. cerevisiae and will differ by 1 non-synonomous/several synonymous changes)
Ssp2 (SSP2 Budding Yeast, Sequence is from the SK1 isolate of S. cerevisiae and may differ by synonymous changes from S288C form)

Tags

glutathione-S-transferase

Expression

Bacterial

Mutation

NdeI and NcoI sites were removed for cloning purp…

Promoter

T7

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1_-FCRL3-50aa-puro

Plasmid

#248153

Purpose

Lentiviral vector encoding a truncated form of human FCRL3, containing only the first 50 residues of the cytoplasmic tail.

Depositor

Silvia Monticelli

Article

Bianchi et al J Exp Med (2026) 223 (1): e20242474.

Insert

FCRL3 (FCRL3 Human)

Use

Lentiviral

Mutation

Mutagenesis to insert a stop codon at aa645 in FC…

Promoter

EF1_

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1_-FCRL3-16aa-puro

Plasmid

#248152

Purpose

Lentiviral vector encoding a truncated form of human FCRL3, containing only the first 16 residues of the cytoplasmic tail.

Depositor

Silvia Monticelli

Article

Bianchi et al J Exp Med (2026) 223 (1): e20242474.

Insert

FCRL3 (FCRL3 Human)

Use

Lentiviral

Mutation

Mutagenesis to insert a stop codon at aa611 in FC…

Promoter

EF1_

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1_-FCRL3-93aa-puro

Plasmid

#248154

Purpose

Lentiviral vector encoding a truncated form of human FCRL3, containing only the first 93 residues of the cytoplasmic tail.

Depositor

Silvia Monticelli

Article

Bianchi et al J Exp Med (2026) 223 (1): e20242474.

Insert

FCRL3 (FCRL3 Human)

Use

Lentiviral

Mutation

Mutagenesis to insert a stop codon at aa688 in FC…

Promoter

EF1_

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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