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AAV-EF1α1.1-FLEX-rc [SomArchon-GFP]

Plasmid

#153532

Purpose

AAV-mediated expression of SomArchon-GFP under the EF1α1.1 promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon-GFP

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Promoter

EF1α1.1

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgSIK3-A

Plasmid

#138682

Purpose

Expresses a mouse SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgSIK3-C

Plasmid

#138683

Purpose

Expresses a mouse SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pTBL649 CHYRON2 integration construct

Plasmid

#126446

Purpose

To integrate the CHYRON2 locus at AAVS1 in human cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6-CHYRON2 hgRNA
pCMV-puro

Expression

Mammalian

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

CMV and human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJC2297 - pHR-TRE3G-hSpCas9-NLS-FLAG-2A-Thy1.1

Plasmid

#250251

Purpose

Expression of Cas9 with Thy1.1 cell surface marker under a doxycycline responsive promoter. Requires additional expression of transactivator.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

hSpCas9

Use

CRISPR and Lentiviral

Tags

NLS-FLAG-2A-Thy1.1

Promoter

TRE3G

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCBS-4804

Plasmid

#249320

Purpose

Expresses Non-targeting sgRNA and contains the Repair Template to replace the KanR* on the genome of E. coli MG1655

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

nontargeting sgRNA

Expression

Bacterial

Mutation

N/A

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pCBS-5740

Plasmid

#249327

Purpose

Expresses sgRNA targeting the fcy gene in S. cerevisiae

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

sgRNA targeting fcy

Expression

Yeast

Mutation

N/A

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pSBtet-FAP-TGN38-GFP

Plasmid

#242992

Purpose

Inducible FAP-TGN38-GFP expression in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

dH6.2-TGN38-GFP (Tgoln2 Rat)

Use

Sleeping beauty

Tags

FAP and GFP

Expression

Mammalian

Promoter

Tight TRE

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSBtet-GFP-TGN38-FAP

Plasmid

#242993

Purpose

Inducible GFP-TGN38-FAP expression in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

GFP-TGN38-dH6.2 (Tgoln2 Rat)

Use

Sleeping beauty

Tags

FAP and GFP

Expression

Mammalian

Promoter

Tight TRE

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSBtet-mCer3-TGN38-FAP

Plasmid

#242994

Purpose

Inducible mCer3-TGN38-FAP expression in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

mCer3-TGN38-dH6.2 (Tgoln2 Rat)

Use

Sleeping beauty

Tags

FAP and mCer3

Expression

Mammalian

Promoter

Tight TRE

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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