We narrowed to 5,913 results for: pcDNA 3.1

1. pcDNA3.1(+)-HaloTag N58-(GGS)X6-c-Jun

   Plasmid

   #171438

   Purpose

   Expression of HaloTag N58-(GGS)X6-c-Jun in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   HaloTag N58-(GGS)X6-c-Jun
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA 3.1(-) mouse C/EBP delta

   Plasmid

   #12559

   Depositor

   Peter Johnson

   Article

   McCauslin et al J Biol Chem. 2006 Jun 30;281(26):17681-8

   Insert

   CCAAT/Enhancer-binding Protein delta (Cebpd Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S2G introduced during cloning

   Promoter

   Available since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 Flag YFP hNKCC1 (NT17)

   Plasmid

   #49085

   Purpose

   Expresses human NKCC1 with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   262 silent mutations from native hNKCC1

   Promoter

   CMV

   Available since

   Nov. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-KozATG-dL5-2XG4S-actin

   Plasmid

   #73262

   Purpose

   Expresses dL5(E52D)-actin fusion within mammalian cells. (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Yan et al Chemphyschem. 2014 Mar 17;15(4):687-95.

   Insert

   KozATG-dL5-2XG4S-actin (ACTB Synthetic, Human)
   

   Use

   Tags

   The FAP and actin are fused with 2 copies of a G4…

   Expression

   Mammalian

   Mutation

   The dL5** FAP is E50D, L89S, also known as E52D/L…

   Promoter

   CMV

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)

   Plasmid

   #158952

   Purpose

   Express human RAD51(F129A) mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

   Insert

   RAD51 (RAD51 Human)
   

   Use

   Tags

   2X FLAG 2X STREP

   Expression

   Mammalian

   Mutation

   changed phenylalanine 129 to alanine

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-2xFLAG-2xSTREP (empty vector)

   Plasmid

   #172604

   Purpose

   Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Type

   Empty backbone

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon

   Plasmid

   #69906

   Purpose

   Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ciRS7 (CDR1-AS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 CARD8-TEV-GFP-FLAG

   Plasmid

   #169991

   Purpose

   Mammalian purification construct for CARD8

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   TEV-GFP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

   Plasmid

   #114111

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100

   Insert

   Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1

    Plasmid

    #86885

    Purpose

    C-terminal part of the split-BioID

    Depositor

    Mathieu Bollen

    Article

    Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

    Insert

    BirA* (AA141-321)- HA- NIPP1]
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    BirA Amino Acids 141-321

    Promoter

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 CMV mCherry-eGFP BGH

    Plasmid

    #92280

    Purpose

    Control FRET construct; tandem mCherry-eGFP fusion

    Depositor

    Baljit Khakh

    Article

    Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

    Insert

    mCherry-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-Pert Heavy chain-SpyTag003

    Plasmid

    #216309

    Purpose

    Expresses anti-HER2 Pert Heavy chain-SpyTag003 in mammalian cells. To be paired with Pert light chain to form the anti-HER2 Pert Fab (related to Pertuzumab)

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    H2-18 Heavy chain-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)/GUG-nLuc-3XFLAG-PEST

    Plasmid

    #127314

    Purpose

    Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-myc-OGA(GS-544)C181_NLS

    Plasmid

    #193997

    Purpose

    plasmid encoding myc-tagged OGA(GS-544)C181_NLS

    Depositor

    Christina Woo

    Article

    Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

    Insert

    myc-OGA(GS-544)C181_NLS
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3.1(+)-hH3-(GGS)X6-HaloTag C58

    Plasmid

    #171432

    Purpose

    Expression of hH3-(GGS)X6-HaloTag C58 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    HaloTag N58-(GGS)X6-hH3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1/FGFR2-S252W(Apert) myc

    Plasmid

    #33249

    Depositor

    Claudio Basilico, Alka Mansukhani

    Article

    Mansukhani et al J Cell Biol. 2000 Jun 12. 149(6):1297-30

    Insert

    FGFR2 (Fgfr2 Mouse)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Mutation S252W in FGFR2 found in Apert syndrome. …

    Promoter

    CMV

    Available since

    Dec. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1 HA-rnSOX9 PQA/S (286-507)

    Plasmid

    #62975

    Purpose

    Expresses HA-tagged rat SOX9 residues 286-507

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    SOX9 (Sox9 Rat)
    

    Use

    Tags

    HA (YPYDVPDY)

    Expression

    Mammalian

    Mutation

    codes for residues 286-507

    Promoter

    CMV

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1(+)-HaloTag N58-(GGS)X6-FRB

    Plasmid

    #171427

    Purpose

    Expression of HaloTag N58-(GGS)X6-FRB in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    HaloTag N58-(GGS)X6-FRB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1/nV5-DEST-beta catenin

    Plasmid

    #20140

    Depositor

    Valeri Vasioukhin

    Article

    Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

    Insert

    pcDNA3.1/nV5-DEST-beta catenin (Ctnnb1 Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    T3I

    Promoter

    CMV

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG WT

    Plasmid

    #163979

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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