2,226 results

1. AAV-Dlx-SynaptoTAG

   Plasmid

   #210729

   Purpose

   AAV vector for mapping synaptic projections. GABAergic neuron-specific Dlx promoter expresses EGFP-Synaptobrevin-2 to label synaptic terminals and Palm-tdTomato to label neuronal soma and axons.

   Depositor

   Wei Xu

   Article

   Du et al Cell Rep Methods. 2023 Jun 16;3(6):10050

   Insert

   EGFP-Synaptobrevin-2, P2A, Palm-tdTomato (tdTomato with Palmitoylation sequence) (Vamp2 Synthetic, Rat)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Dlx

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKT-CNP-inact

   Plasmid

   #211804

   Purpose

   Expresses inactive 2',3'-cyclic nucleotide phosphodiesterase catalytic domain (catalytic residues mutated)

   Depositor

   Emily Weinert

   Article

   Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

   Insert

   2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H73L H153L

   Promoter

   tet

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKT-CNP

   Plasmid

   #211803

   Purpose

   Expresses 2',3'-cyclic nucleotide phosphodiesterase catalytic domain

   Depositor

   Emily Weinert

   Article

   Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

   Insert

   2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tet

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET30b jGCaMP8f L27A

   Plasmid

   #204138

   Purpose

   Production of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coli

   Depositor

   Katalin Torok

   Article

   Tran et al Front Cell Neurosci. 2023 May 18;17:1155

   Insert

   jGCaMP8f L27A
   

   Use

   Tags

   N-terminal His tag

   Expression

   Bacterial

   Mutation

   Leucine 27 changed to Alanine

   Promoter

   T7

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGP-CMV-jGCaMP8f L27A

   Plasmid

   #204140

   Purpose

   Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutation

   Depositor

   Katalin Torok

   Article

   Tran et al Front Cell Neurosci. 2023 May 18;17:1155

   Insert

   jGCaMP8f L27A
   

   Use

   Tags

   N-terminal His tag

   Expression

   Mammalian

   Mutation

   Leucine 27 changed to Alanine

   Promoter

   CMV

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. dCas9-RPT6-FLAG

   Plasmid

   #205416

   Purpose

   Expresses dCas9-RPT6 with FLAG label

   Depositor

   Timothy Jarome

   Article

   Farrell et al J Neurosci. 2024 Jan 24;44(4):e145323202

   Insert

   dCas9-RPT6 (Psmc5 Rat)
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. An R57A, R59A CAAX pMT2

   Plasmid

   #206119

   Purpose

   Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif

   Depositor

   Annette Dolphin

   Article

   Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

   Insert

   cacna1a (Cacna1a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R57A, R59A

   Promoter

   Ad MLP/TPL/SV40

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. cacna2d1 R241A pMT2

   Plasmid

   #206110

   Purpose

   expression of rat alpha2delta-1 calcium channel auxillary subunit and with R421A mutation that disrupts Gabapentin binding

   Depositor

   Annette Dolphin

   Article

   Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

   Insert

   cacna2d1 (Cacna2d1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R241A

   Promoter

   Ad MLP/TPL/SV40

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. cacna2d1 R241A HA pMT2

   Plasmid

   #206111

   Purpose

   expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with an R421A mutation that disrupts Gabapentin binding

   Depositor

   Annette Dolphin

   Article

   Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

   Insert

   cacna2d1 (Cacna2d1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R241A

   Promoter

   Ad MLP/TPL/SV40

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. An HA CAAX pMT2

    Plasmid

    #206116

    Purpose

    expression of Cav2.1 N-terminus (amino acids 1-100) with a C-terminal CAAX (last 10aa of H.Ras) motif and a HA tag

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ad MLP/TPL/SV40

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. cacna2d1 BBS pcDNA3

    Plasmid

    #206106

    Purpose

    expression of rat alpha2delta-1 calcium channel auxillary subunit with a single Bungarotoxin Binding Site motif inserted after aa549HA

    Depositor

    Annette Dolphin

    Article

    Meyer et al Sci Rep. 2021 May 13;11(1):10256. doi: 1

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Cav2.1 R57A, R59A pMT2

    Plasmid

    #206112

    Purpose

    expression of rat Cav2.1 calcium channel with R57A and R59A mutations that prevent G-protein modulation

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Neurobiol Dis. 2016 Sep;93:243-56. doi:

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R57A, R59A

    Promoter

    Ad MLP/TPL/SV40

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. rUBR5_2383-2442_pGEX6P1

    Plasmid

    #209791

    Purpose

    Bacterial expression of UBR5 Mlle domain

    Depositor

    Kalle Gehring

    Article

    Lim et al J Biol Chem. 2006 May 19;281(20):14376-8

    Insert

    Mlle domain of UBR5 (Ubr5 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 2383-2442 only

    Promoter

    tac

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pETSyt1C2AB(80-421)msGFP

    Plasmid

    #209009

    Purpose

    Recombinant protein expression of truncated syt1 tagged with GFP

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Tags

    6xHis and msfGFP

    Expression

    Bacterial

    Mutation

    aa 80-421 only

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETSUMOSyt1C2AB(96-421)

    Plasmid

    #209006

    Purpose

    Recombinant protein expression of truncated syt1 construct

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    aa 96-421 only

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETmsGFPSyt1(80-142)

    Plasmid

    #209012

    Purpose

    Recombinant protein expression of truncated syt1 tagged with GFP

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Tags

    6xHis-msfGFP

    Expression

    Bacterial

    Mutation

    aa 80-142 only

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETmsGFPSyt1(80 JuxtaK-142)

    Plasmid

    #209013

    Purpose

    Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Tags

    6xHis-msfGFP

    Expression

    Bacterial

    Mutation

    aa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETSyt1C2AB(143-421)msGFP

    Plasmid

    #209010

    Purpose

    Recombinant protein expression of truncated syt1 tagged with GFP

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Tags

    6xHis and msfGFP

    Expression

    Bacterial

    Mutation

    aa 143-421 only

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETSUMOSyt1C2AB(80 JuxtaK-421)

    Plasmid

    #209008

    Purpose

    Recombinant protein expression of truncated syt1 construct with Juxta K mutations

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFSynWSyt1C2AB(80 JuxtaK-421)msGFP

    Plasmid

    #209016

    Purpose

    Overexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cells

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge