We narrowed to 7,125 results for: ef1a
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Plasmid#111581PurposeAn AAV vector that expresses double floxed myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.DepositorInsertmyrTagRFP-T-P2A-post-eGRASP
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP
Plasmid#111585PurposeAn AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.DepositorInsertmyriRFP670V5-P2A-post-eGRASP
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF1α:flex-mcherry
Plasmid#222618PurposeCre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoterDepositorInsertCre-dependent mCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-mRuby3-MLC-IRES-Neo
Plasmid#222635PurposeReporter for activated NM2DepositorInsertmRuby3-MLC (MYL9 Human)
UseLentiviralTagsmRuby3ExpressionMutationPromoterEF1aAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCaMKII-EBFP-tTAn
Plasmid#172124PurposeExpression of EBFP and tTA-InteinN in CaMKII positive cellsDepositorInsertpCaMKII, EBFP, tTAn-InteinN
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
LADL Anchor
Plasmid#127664PurposeEncodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoterDepositorInsertdCas9-CIBN-2A-Puro
UseTags3XFLAGExpressionMammalianMutationCas9 (D10A,H840A) mutantPromoterEF1aAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
AYBEv0.1
Plasmid#193966PurposeA wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoterDepositorInsertecTadA8e-nSpCas9(D10A)-MPG(wt)
UseTagsExpressionMammalianMutationPromoterhU6, EF1a, CBhAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-cFos-mCh
Plasmid#193066Purposeconstitutive expression of mouse cFos fused to mCherry in mammalian cellsDepositorInsertcfos-mCh
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-Gata2-mCh
Plasmid#193064Purposeconstitutive expression of mouse Gata2 fused to mCherry in mammalian cellsDepositorInsertGata2-mCh
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-Gfi1b-mCh
Plasmid#193065Purposeconstitutive expression of mouse Gfi1b fused to mCherry in mammalian cellsDepositorInsertGfi1b-mCh
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDY0973-RADARS iCaspase
Plasmid#182539PurposeExpress RADARS iCaspase that conditionally turn on iCaspase in the presence of target RNADepositorInsertiCaspase9
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_4
Plasmid#192688PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_1
Plasmid#192685PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_2
Plasmid#192686PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_3
Plasmid#192687PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiMPH v2
Plasmid#89308Purposelenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.DepositorInsertMS2-P65-HSF1_2A_Hygro (RELA Human, Synthetic)
UseLentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1aAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
phDLX2-N174
Plasmid#60860Purpose2nd generation lentiviral transfer plasmid. Expresses DLX2 under the EF1a promoterDepositorInsertDLX2 (DLX2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
Human USP7-3X FLAG
Plasmid#225334PurposeLentiviral expression of human USP7 with 3x FLAG tag in mammalian cellsDepositorInsertUSP7 (USP7 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human USP7_H456A-3X FLAG
Plasmid#225335PurposeLentiviral expression of human mutant USP7 with 3x FLAG tag in mammalian cellsDepositorInsertUSP7 (USP7 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationH456APromoterEF1aAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
AYBEv3
Plasmid#193967PurposeAn engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoterDepositorInsertecTadA8e-nSpCas9(D10A)-MPG(v3)
UseTagsExpressionMammalianMutationG163R, N169S, S198A, K202A, G203A, S206A, K210APromoterhU6, EF1a, CBhAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only