We narrowed to 2,015 results for: D10

Showing: 1841 - 1860 of 2015 results

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pAG415Gal_Gcn4 Aro3+HA

Plasmid

#232990

Purpose

Galactose iduced expression of Gcn4 Aro3+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 solvvol W

Plasmid

#231878

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 solvvol W

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 solvvol W

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 solvvol

Plasmid

#231877

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 solvvol

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 solvvol

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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Stim1NTD-ABHD16Acyto-mNG

Plasmid

#195158

Purpose

mammalian expression of ER localized ABHD16A tagged with mNeonGreen

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

Stim1NTD (STIM1 Human)

Tags

ABHD16A cytoplasmic domain and mNeonGreen

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pIG-713pUC19-tNGFR-P2A-CD45

Plasmid

#186109

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CD45 (PTPRC Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pNOC_dCas9_sgRNA Td2

Plasmid

#176258

Purpose

pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

dead spCas9

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Mutation

H840A and D10A mutations on spCas9 to inactivate …

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pJK434

Plasmid

#155378

Purpose

PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus
PphlF_A4NT in dCas9 sgRNA oscillator v1

Use

CRISPR

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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J364B_AttB_mkozak_STIM1_IRES_CaMPARI2-P2A-PuroR

Plasmid

#232939

Purpose

Low expression plasmid of STIM1 with fluorescent calcium biosensor CaMPARI2 for Matreyek Bxb1(GT) landing pad

Depositor

Kenneth Matreyek

Article

Kamath et al Genetics. 2025 Jul 30:iyaf146. doi: 10.1

Inserts

STIM1 (STIM1 Human)
CaMPARI2

Expression

Mammalian

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro3+GFP

Plasmid

#233002

Purpose

Galactose iduced expression of Gcn4 Aro3+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A-SNAPf-HisTag-FLAG

Plasmid

#200789

Purpose

Mammalian expression of CLIPf-KIF5A-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al iScience. 2026 Feb 2;29(3):114875. doi:

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1_dCas9-XTEN-KRAB-p2A-GFP

Plasmid

#222108

Purpose

Donor vector for CRISPRi integration at AAVS1 with a GFP fluorophore

Depositor

Jonathan Weissman

Article

She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

Insert

CRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)

Use

AAV and CRISPR

Tags

HA and P2A-GFP

Expression

Mammalian

Mutation

D10A, H840A

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-TNFRSF9-COMP5AP-AviTag-9xHis

Plasmid

#157121

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TNFRSF9 (TNFRSF9 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-STIM1(238-685)

Plasmid

#248297

Purpose

An expression construct encoding an EGFP-fused STIM1 fragment (a.a.238-685).

Depositor

Sangkyu Lee

Article

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

EGFP-fused STIM1 fragment (a.a.238-685) (STIM1 )

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-237

Promoter

CMV

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pNK036C_AttB_mkozak_mGreenLantern-STIM1_IRES_mCherry-H2A-P2A-PuroR

Plasmid

#232938

Purpose

Low expression plasmid of STIM1 with N-terminal mGreenLantern tag for Matreyek Bxb1 (GT) landing pad

Depositor

Kenneth Matreyek

Article

Kamath et al Genetics. 2025 Jul 30:iyaf146. doi: 10.1

Insert

STIM1 (STIM1 Human)

Tags

mGreenLantern

Expression

Mammalian

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pUHD 10-3-human CDK11 p110B wt.-FLAG

Plasmid

#240138

Purpose

Express CDK11 p110B gene

Depositor

Vincent Kidd, Jill Lahti, Martine Roussel

Article

Loyer et al J Biol Chem. 2008 Mar 21;283(12):7721-32

Insert

CDK11 p110 (CDK11A Human)

Tags

FLAG

Expression

Mammalian

Promoter

TRE, CMV

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1-DSBH pc2335

Plasmid

#201697

Purpose

Expresses of GFP-tagged mTet1-DSBH in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1 DSBH (Tet1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-EA-evoAPOBEC1max_NG-BlastR

Plasmid

#232721

Purpose

C-to-T base editor

Depositor

David Liu

Article

Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

Insert

evoAPOBEC1max H47E+S48A-nCas9(NG)-UGI-UGI

Expression

Mammalian

Mutation

within evoAPOBEC1: H47E+S48A; within Cas9 D10A

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-mCherry-LAMP1-Y414A

Plasmid

#222320

Purpose

Synchronize the trafficking of LAMP1-A17 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1-Y414A fused to SBP-mCherry (LAMP1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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TH1202-pOCC175_pOEM1-N-HIS-MBP-PS-mGFP-TEV-HNRNPA3_opt

Plasmid

#221890

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

HNRNPA3 (HNRNPA3 Human)

Tags

6xHis-MBP-PreScission-mGFP-TEV

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFBD-BirA_ATG16L1:303-607

Plasmid

#210942

Purpose

Insect expression of human ATG16L1 fragment G303 to Y607. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

ATG16L1:G303-Y607 (ATG16L1 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 4, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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