We narrowed to 165,343 results for: Gene

1. pNSA-ARS-CRISPR-BlastR

   Plasmid

   #101009

   Purpose

   Expresses Cas9-Nlux and sgRNA and contains CEN/ARS. CCMP537 LDSP promoter driven Blasticidin resistance gene.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAGM51559

   Plasmid

   #153226

   Purpose

   Control plasmid for knockout of TRY and CPC genes

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Insert

   Cas9, guide RNA for AP3, FAST marker
   

   Use

   Synthetic Biology

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKL2013

   Plasmid

   #172182

   Purpose

   A CRISPR/Cas9 vector targeting maize glossy2 gene for targeted mutagenesis with a mCherry as fluorescent marker.

   Depositor

   Kan Wang

   Article

   McCaw et al Front Genome Ed. 2021 Jan 11;2:622227. d

   Insert

   mCherry
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Cauliflower mosaic virus 35S promoter

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCRISPaint-T2A-sfGFP-3xP3-RFP

   Plasmid

   #127557

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   sfGFP
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pILGFPE9

   Plasmid

   #83545

   Purpose

   Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   TEF1 promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMaCTag-P33

   Plasmid

   #120018

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with FLAG

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   FLAG
   

   Use

   Pcr template

   Tags

   FLAG

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TrpLE-M-nMSP1E3D1

   Plasmid

   #179949

   Purpose

   Tagless membrane scaffold protein (MSP) for formation of nanodiscs.

   Depositor

   Kerney Glover

   Article

   Julien et al Anal Biochem. 2021 Nov 27:114497. doi: 1

   Insert

   TrpLE-M-nMSP1E3D1
   

   Tags

   TrpLE

   Expression

   Bacterial

   Mutation

   Native Trp residues have been mutated to Phe resi…

   Promoter

   T7

   Available Since

   Feb. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMBA328

   Plasmid

   #214748

   Purpose

   Medium copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)

   Depositor

   Tae Seok Moon

   Article

   Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   BBa_J23119-RBS(22821)-mcherry
   

   Expression

   Bacterial

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMBA333

   Plasmid

   #214745

   Purpose

   Medium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

   Depositor

   Tae Seok Moon

   Article

   Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   BBa_J23119-RiboJ-RBS(21992)-gfpmut3
   

   Expression

   Bacterial

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMaCTag-07

    Plasmid

    #124786

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mNeonGreen
    

    Use

    Pcr template

    Tags

    mNeonGreen

    Promoter

    None

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-nDsRedFL-intron

    Plasmid

    #177804

    Purpose

    An intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    intron
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMaCTag-Z22

    Plasmid

    #120065

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    SNAP
    

    Use

    Pcr template

    Tags

    SNAP

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMaCTag-P27

    Plasmid

    #120038

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 1xHA

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    1xHA
    

    Use

    Pcr template

    Tags

    1xHA

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPaint-T2A-FLPo-3xP3-RFP

    Plasmid

    #127561

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    FLPo
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPaint-T2A-Gal80-3xP3-RFP

    Plasmid

    #127562

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Gal80
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMSP2

    Plasmid

    #26282

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP2
    

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    synthetic gene of dimer of deletion mutant (1-43)…

    Available Since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pMaCTag-Z34

    Plasmid

    #120062

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xFLAG

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    3xFLAG
    

    Use

    Pcr template

    Tags

    3xFLAG

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMOD_D-CmYLCV-NbGP41P-sfGFP-VP64-GB1

    Plasmid

    #202010

    Purpose

    Moclo level 1 - Module D, Promoter: CmYLCV, Gene: NbGP41P-sfGFP-VP64-GB1, Terminator: ZmUBI

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Insert

    NbGP41P-sfGFP-VP64-GB1
    

    Use

    Synthetic Biology; Moclo level 1 vector

    Tags

    GB1 and sfGFP

    Promoter

    CmYLCV

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 221-AID-RPB1

    Plasmid

    #195108

    Purpose

    Targeting vector to introduce AID tag in N-terminal of the human RPB1 gene. Auxin-inducible degron system.

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    AID
    

    Expression

    Mammalian

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMaCTag-P12

    Plasmid

    #120023

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mScarlet
    

    Use

    Pcr template

    Tags

    mScarlet

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge