We narrowed to 5,710 results for: KRAS

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pPB-cT3G-cERP2-SOX17

Plasmid

#222925

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SOX17

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

SOX17 (SOX17 Human)

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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AA390

Plasmid

#215957

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control reporter

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; sgEGFP_v1 [Sp]; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA246

Plasmid

#215946

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; sgCD55_v1 [Sp]; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA233

Plasmid

#215941

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; sgCD274_v1 [Sp]; trRNA_v14 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA244

Plasmid

#215945

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; sgCD59_v1 [Sp]; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA363

Plasmid

#215955

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

dU6:3_v1; BsmBI_v14; BsmBI_v1; trRNA_v1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA303

Plasmid

#216071

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; BsmBI_v0; BsmBI_v6; trRNA_v1 [Nme2]; terminator_v1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA242

Plasmid

#215943

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; sgCD47_v1 [Sp]; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA243

Plasmid

#215944

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; sgCD47_v5 [Sp]; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA310

Plasmid

#215950

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control lethal

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; sgPLK1_v2 [Sp]; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA232

Plasmid

#215940

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; sgCD47_v4 [Sp]; trRNA_v14 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA238

Plasmid

#215942

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; sgCD47_v4 [Sp]; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA054

Plasmid

#215932

Purpose

Fragmid fragment: (guide cassette) guide expression; reverse orientation

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

rev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v3}

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA269

Plasmid

#216073

Purpose

Fragmid fragment: (CROPseq) WPRE; 3'LTR; guide expression bGHpolyA

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

WPRE_v1; U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]; 3'LTR; bGHpolyA_v1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA285

Plasmid

#215947

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; sgCD81_v1; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AA023

Plasmid

#215931

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; sgCD81_v1 [Sp]; trRNA_v2 [Sp]; CS2

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AA022

Plasmid

#215930

Purpose

Fragmid fragment: (guide cassette) 2xPP7 & 2xMS2 in trRNA

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; sgCD4_v3 [Sp]; trRNA_v14 [Sp]; CS2

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AA016

Plasmid

#215927

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v3

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AA017

Plasmid

#215928

Purpose

Fragmid fragment: (guide cassette) 2xPP7 & 2xMS2 in trRNA

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v14 [Sp]; CS2

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AA102

Plasmid

#215935

Purpose

Fragmid fragment: (guide cassette) guide expression; reverse orientation; enables iBar UMIs

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

rev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5}

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDB_051

Plasmid

#216081

Purpose

for stable fly cell lines; attL and attR sequences for genome integration by phiC31, destination vector

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

attL and attR sequences for genome integration by phiC31

Use

Destination

Expression

Insect

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA107

Plasmid

#215936

Purpose

Fragmid fragment: (guide cassette) guide expression; enables iBar UMIs

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMV_AA091

Plasmid

#216106

Purpose

CRISPRa, gRNA expression with modified trRNA to recruit activators via PP7 tags (guide only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

gRNA expression with modified trRNA to recruit activators via PP7 tags

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K48R)-GFP

Plasmid

#212793

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K48R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K48R)-GFP

Use

Integrative vector

Expression

Yeast

Mutation

CUb(K48R)

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K63R)-GFP

Plasmid

#212794

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K63R)-GFP

Use

Integrative vector

Expression

Yeast

Mutation

CUb(K63R)

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-NUbo-E3(48) aa24-150-VSV

Plasmid

#212747

Purpose

Constitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48) aa24-150

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Cue1 ΔU7BD

Promoter

ADH1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-myc-E3(1) C885A-CUbo-VSV

Plasmid

#212738

Purpose

Consitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-NLS-VSV and myc

Expression

Yeast

Mutation

HOIP(C885A)

Promoter

ADH1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

Plasmid

#212805

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

Expression

Mammalian

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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PET-HypaR-SpCas9-NLS-6xHis

Plasmid

#207387

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HypaR-SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R661A, N692A, M694A, Q695A, H698A

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PET-SpCas9-HF1-NLS-6xHis

Plasmid

#207382

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

SpCas9-HF1

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge