We narrowed to 15,034 results for: pET

1. pJK044 (pET-PurQ-wt)

   Plasmid

   #73839

   Purpose

   Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)

   Depositor

   T. J. Kappock, JoAnne Stubbe

   Article

   Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

   Insert

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM6-1D4-mCherry

   Plasmid

   #73423

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 1D4 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P1D4 (orthogonal T7-lac variant)

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM6-4A6-mCherry

   Plasmid

   #73424

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 4A6 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P4A6 (orthogonal T7-lac variant)

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM6-1E4-mCherry

   Plasmid

   #73426

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 1E4 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P1E4 (orthogonal T7-lac variant)

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM6-1B6-mCherry

   Plasmid

   #73420

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 1B6 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P1B6 (orthogonal T7-lac variant)

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM6-3H5-mCherry

   Plasmid

   #73419

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 3H5 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P3H5 (orthogonal T7-lac variant)

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET23a-MmUBB(SnaBI)

   Plasmid

   #69558

   Purpose

   In vitro transcription/translation (T7)

   Depositor

   Jorge Eduardo Azevedo

   Article

   Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

   Insert

   UBB (Ubb Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The two last codons (TATTAA; encoding the last ty…

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pET23a-MmUBB(Y305P)

   Plasmid

   #69557

   Purpose

   Bacterial expression; in vitro transcription/translation (T7)

   Depositor

   Jorge Eduardo Azevedo

   Article

   Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

   Insert

   UBB (Ubb Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon TAT encoding tyrosine 305 was changed to CC…

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pET151-hUCH37 (ISF2)

   Plasmid

   #61930

   Purpose

   Expression of hUCH37 (ISF2) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Insert

   hUCH37 (ISF1) (UCHL5 Human)
   

   Use

   Tags

   6x HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pET30Xa-SBL1(160E)

    Plasmid

    #66846

    Purpose

    Expresses soybean lipoxygenase-1 S160E mutant with N-term Histag

    Depositor

    Betty Gaffney

    Article

    Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

    Insert

    soybean lipoxygenase-1 (LOC547694, NEWENTRY Glycine max)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    S160E

    Promoter

    T7lac

    Available Since

    Sept. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET11+PCNA3-CYP119

    Plasmid

    #66136

    Purpose

    Expresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15b+PCNA3 T180C

    Plasmid

    #66181

    Purpose

    Expresses the T180C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    T180C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b+PCNA3 R112C

    Plasmid

    #66180

    Purpose

    Expresses the R112C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b+PCNA2 E105C

    Plasmid

    #66176

    Purpose

    Expresses the E105C mutant of Sulfolobus solfataricus PCNA2

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 2
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E105C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-p53 (Cow 1-82)

    Plasmid

    #62062

    Purpose

    Expression of Cow p53 (residues 1-82) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-82

    Promoter

    T7

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pET28-p53 (1-73) (K24N)

    Plasmid

    #62079

    Purpose

    Expression of human p53 (1-73) (K24N) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Zhan et al Proteins. 2013 Oct;81(10):1738-47. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; K24N

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pET28-p53 (Rabbit 1-87)

    Plasmid

    #62058

    Purpose

    Expression of Rabbit p53 (residues 1-87) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-87

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pET28A-p53 (1-73) (PPP)

    Plasmid

    #62067

    Purpose

    Expression of human p53 (1-73) (P12A, P13A, P27A) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains TAD residues 1-73; P12A, P13A, P27A

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pET28-ceRab2(1-214)

    Plasmid

    #59550

    Purpose

    makes 6x Fusion of Full length ceRab2

    Depositor

    David Lambright

    Article

    Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

    Insert

    1-214
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pET28-ceRab5(1-208)

    Plasmid

    #59551

    Purpose

    makes 6x Fusion of Full length ceRab5

    Depositor

    David Lambright

    Article

    Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

    Insert

    1-208
    

    Use

    Tags

    6x His

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pET28-ceRab10(1-201)

    Plasmid

    #59552

    Purpose

    makes 6x Fusion of Full length ceRab10

    Depositor

    David Lambright

    Article

    Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

    Insert

    1-208
    

    Use

    Tags

    6x His

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pET28-ceRab11(1-211)

    Plasmid

    #59553

    Purpose

    makes 6x Fusion of Full length ceRab11

    Depositor

    David Lambright

    Article

    Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

    Insert

    1-211
    

    Use

    Tags

    6x His

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pET28-ceRab21(1-207)

    Plasmid

    #59554

    Purpose

    makes 6x Fusion of Full length ceRab21

    Depositor

    David Lambright

    Article

    Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

    Insert

    1-207
    

    Use

    Tags

    6x His

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pET28-ceRab21(1-209)

    Plasmid

    #59555

    Purpose

    makes 6x Fusion of Full length ceRab35

    Depositor

    David Lambright

    Article

    Chotard et al Mol Biol Cell. 2010 Jul 1;21(13):2285-96

    Insert

    1-209
    

    Use

    Tags

    6x His

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pET-20b-T7-Ntx

    Plasmid

    #58714

    Purpose

    Expresses T7-Notexin in Escherichia coli

    Depositor

    Fiorella Tonello

    Article

    Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

    Insert

    Notexin
    

    Use

    Tags

    T7 peptide tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET14b-PITPalpha N90F

    Plasmid

    #58257

    Purpose

    bacterial expression of human PITP alpha N90F

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    N90F (abolishes PI binding)

    Promoter

    T7

    Available Since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pET14b-PITPalpha K61A

    Plasmid

    #58258

    Purpose

    bacterial expression of human PITP alpha K61A

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K61A (abolishes PI binding)

    Promoter

    T7

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pET29b-ClbP-S95A

    Plasmid

    #48245

    Purpose

    Untagged, full-length protein with active site mutation Serine95 to Alanine for in vivo peptidase assays

    Depositor

    Emily Balskus

    Article

    Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

    Insert

    ClbP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed Serine 95 to Alanine

    Promoter

    T7

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-28a-CylI

    Plasmid

    #48200

    Purpose

    Expresses CylI (type III PKS) in E. coli

    Depositor

    Emily Balskus

    Article

    Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

    Insert

    CylI
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-29b-CylB

    Plasmid

    #48199

    Purpose

    Expresses CylB (acyl carrier protein) in E. coli

    Depositor

    Emily Balskus

    Article

    Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

    Insert

    CylB
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge