We narrowed to 8,358 results for: BLI;

1. pSNAP-CaaX Control Plasmid

   Plasmid

   #101133

   Purpose

   Expression of SNAP-Tag and CaaX box (Farnesylation Signal) in Mammalian cells

   Depositor

   Ana Egana, New England Biolabs

   Insert

   CaaX Box
   

   Use

   Tags

   SNAP-tag (SNAP26m)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. Mek2

   Plasmid

   #40776

   Depositor

   Dustin Maly

   Insert

   MKK2 (MAP2K2 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Starting Met removed

   Promoter

   CMV

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

3. alpha5-EGFP

   Plasmid

   #118956

   Purpose

   GABAA receptor expression (alpha5 subunit). EGFP tag at the C-terminus.

   Depositor

   Angel de Blas

   Insert

   GABRA5 (Gabra5 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEW-pHluorin2-PTS1

   Plasmid

   #213776

   Purpose

   Expresses the fluorescent protein pH sensor pHlorin2 in the glycosomes of Trypanosomal brucei

   Depositor

   Kenneth Christensen

   Insert

   pHlorin2
   

   Use

   Trypanosoma brucei expression

   Tags

   Expression

   Mutation

   Appended a 2 glycine linker and a T. brucei perox…

   Promoter

   T. brucei rRNA promotor

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.3_SARS2_BA.2.86

   Plasmid

   #208441

   Purpose

   expressing SARS-CoV-2 BA.2.86 spike protein for pseudovirus production

   Depositor

   David Nemazee

   Insert

   Spike (BA.2.86) (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   see GISAID sequence

   Promoter

   CMV

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQCXIP-INHBA

   Plasmid

   #73015

   Purpose

   Retroviral expression of human activin A

   Depositor

   Michael Grusch

   Insert

   INHBA (INHBA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGFP FAK Y397F

   Plasmid

   #50516

   Purpose

   This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

   Depositor

   Kenneth Yamada

   Insert

   protein tyrosine kinase, mutated
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Y397F

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pLV-DBNF-mCherry

   Plasmid

   #187195

   Purpose

   To express the mouse BDNF tagged with the FP mCherry

   Depositor

   Pantelis Tsoulfas

   Insert

   BDNF
   

   Use

   Lentiviral

   Tags

   fluorescent protein mCherry

   Expression

   Mammalian

   Mutation

   The codons have been optimized for better express…

   Promoter

   CMV

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p14-9VN

   Plasmid

   #50930

   Purpose

   Expresses human SRP14 and SRP9 proteins as a fusion protein in mammalian cells.

   Depositor

   Katharina Strub

   Insert

   SRP14 and SRP9 (SRP14, SRP9 Human)
   

   Use

   Tags

   VN tag and flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PTPRB

    Plasmid

    #39165

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTPRB (PTPRB Human)
    

    Use

    Tags

    GST-PreScission

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pFLAG-6a-Ataxin3Q80

    Plasmid

    #22129

    Depositor

    Henry Paulson

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pScarlessHD-2xHA-DsRed

    Plasmid

    #80822

    Purpose

    Vector containing a cassette for scarless insertion of 2xHA and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Kate O'Connor-Giles

    Insert

    2xHA-piggyBac-DsRed
    

    Use

    Unspecified

    Tags

    2xHA

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcU6.3_gRNA_c1

    Plasmid

    #221381

    Purpose

    Chicken-specific U6 sgRNA expression mini-vector with 10x capture sequence 1

    Depositor

    Tatjana Sauka-Spengler, James Briscoe

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    G. gallus U6.3

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUS252

    Plasmid

    #127674

    Purpose

    Expresses fuGFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFP to place in other plasmid backbones

    Depositor

    Nicholas Coleman

    Insert

    Free Use GFP (fuGFP)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    June 24, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAAV-Ef1a-vCreDIO hChR2(H134R)-eYFP 2.0

    Plasmid

    #126081

    Purpose

    vCre-Dependent ChR2(H134R)-eYFP with the vLox sites in the reverse orientation

    Depositor

    Karl Deisseroth

    Insert

    hChR2(H134R)-eYFP
    

    Use

    AAV

    Tags

    eYFP

    Expression

    Mutation

    H134R

    Promoter

    Ef1a

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)

    Plasmid

    #176932

    Purpose

    Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hGEM(1-110)_mNG_hSLBP(18-108)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK1

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCGN-MEF2D

    Plasmid

    #32963

    Purpose

    For mammalian expression of mouse MEF2D with an HA tag

    Depositor

    Ron Prywes

    Insert

    MEF2D (Mef2d Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E287G

    Promoter

    CMV

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLAG-hMYOD1 (#794)

    Plasmid

    #78329

    Purpose

    Overexpression of human MYOD1 ORF with a N-terminal FLAG tag

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    MYOD1 (MYOD1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

19. YWHAE

    Plasmid

    #39146

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    YWHAE (YWHAE Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. AAVS1-SAGFP

    Plasmid

    #127100

    Purpose

    SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.

    Depositor

    Adam Karpf

    Insert

    SA-GFP reporter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNAI Neo human PECAM FL

    Plasmid

    #42902

    Depositor

    Steven Albelda

    Insert

    PECAM (PECAM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2013

    Availability

    Academic Institutions and Nonprofits only

22. pAP84

    Plasmid

    #86554

    Purpose

    Expresses sLucopt luciferase from the constitive Bacillus subtilis promoter Pveg in Clostridium difficile

    Depositor

    Wiep Klaas Smits

    Insert

    Pveg-sLucopt
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pveg

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. PSIP1

    Plasmid

    #40744

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PSIP1 (PSIP1 )
    

    Use

    Tags

    6xHis TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

24. pDON-AI-Npm2(N533)

    Plasmid

    #51475

    Purpose

    Express mouse phosphorylation-mimicking Npm2 (p-Npm, N533)

    Depositor

    Shunsuke Ishii

    Insert

    Nucleoplasmin 2 (Npm2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Eleven codons (5, 6, 7, 8, 10, 82, 95, 104, 184, …

    Promoter

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCMV-Neo-Bam E2F3b

    Plasmid

    #37975

    Depositor

    Jacqueline Lees

    Insert

    E2F3b (E2F3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

26. ER-SPLICSs-cFAST10

    Plasmid

    #210431

    Purpose

    Expresses ER-targeted split-CFAST10 for Short-Range ER-Mito Contact sites

    Depositor

    Tito Calì

    Insert

    ER-SPLICSs-cFAST10
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDual_dsCas9_Blast_hs_NC_chr1

    Plasmid

    #214690

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

    Depositor

    Kevin Janes

    Insert

    dgRNA_Chr1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDual_dsCas9_Blast_hs_TP73

    Plasmid

    #214691

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human TP73

    Depositor

    Kevin Janes

    Insert

    dgRNA_TP73 (TP73 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. lentiCRISPRv2_PROX1_1+3

    Plasmid

    #121176

    Purpose

    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

    Depositor

    Timo Otonkoski

    Insert

    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP

    Plasmid

    #52053

    Purpose

    Used in phosphoprotein synthesis. Replace the sfGFP with gene of interest (GOI). Add TAG amber codon(s) at the position(s) in GOI where phosphoserine incorporation is desired.

    Depositor

    Jesse Rinehart

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetR/pLtetO

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pML104-NatMx3

    Plasmid

    #83477

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    NatMx3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

32. pcDNA3.1-Letm1

    Plasmid

    #191962

    Purpose

    Expression of Letm1 (Leucine Zipper And EF-Hand Containing Transmembrane Protein 1).

    Depositor

    Luca Scorrano

    Insert

    Letm1 (LETM1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSBBi-Pur LaG17-synNotch-TetRVP64

    Plasmid

    #158133

    Purpose

    Constitutive expression of anti-GFP nanobody (LaG-17) synNotch TetRVP64 receptor in a pSBBi-Pur Sleeping Beauty backbone

    Depositor

    Neal Devaraj

    Insert

    LaG17-synNotch-TetRVP64
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CMV-dCas9_puroR

    Plasmid

    #219825

    Purpose

    CMV-driven expression of dCas9 with puromycin resistance (Adapted from plasmid #68416)

    Depositor

    Philipp Maass

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    CMV

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA-Dlx6

    Plasmid

    #99480

    Purpose

    Mammalian expression of the Distal-less Homeobox 6 gene

    Depositor

    Jhumku Kohtz

    Insert

    Dlx6 (Dlx6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLV V5-lamin B2

    Plasmid

    #175109

    Purpose

    Lentiviral expression of V5-tagged mouse Lmnb2

    Depositor

    Larry Gerace

    Insert

    Lmnb2 (Lmnb2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SLC9A3R2-TwinStrepII

    Plasmid

    #83200

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Insert

    SLC9A3R2 (Slc9a3r2 Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSBbi-Pur/GDI1-splitTurboID

    Plasmid

    #198219

    Purpose

    Transposon vector (Addgene #60523) for expression of part of TurboID (up to E256) and GID1, for gibberellic acid-inducible biotin labeling

    Depositor

    Eric Davis

    Insert

    GID1
    

    Use

    Transposon

    Tags

    5’ part of TurboID, up to E256

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. YWHAQ

    Plasmid

    #38931

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    YWHAQ (YWHAQ Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

40. pE-SUMO CRYAA

    Plasmid

    #80756

    Purpose

    Expression of human alpha A-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYAA (CRYAA Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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