Skip to main content
Addgene

We narrowed to 4,188 results for: Pet2

Showing: 1861 - 1880 of 4188 results
  1. pET28a-HIS-TagGFP2-TEV-GGG-ELP(40nm)-Cys

    Plasmid
    #90474
    Purpose
    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal TagGFP2 and TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine
    Depositor
    Insert
    TagGFP2-TEV-GGG-ELP(40nm)-Cys
    Use
    Tags
    Cysteine and Sortase-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)

    Plasmid
    #153441
    Purpose
    E. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Insert
    Rc. XDocB binding mode B knock-out mutant
    Use
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Arginine 140 and methionine 144 were mutated to a…
    Promoter
    T7 promoter
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMA-KO)

    Plasmid
    #153440
    Purpose
    E. coli expression of Rc. XDocB binding mode A knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Insert
    Rc. XDocB binding mode A knock-out mutant
    Use
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Arginine 191 and leucine 195 on XDocB were mutate…
    Promoter
    T7 promoter
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His

    Plasmid
    #222288
    Purpose
    Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal GFP and His tag in E.coli for purification.
    Depositor
    Insert
    KIF1C (KIF1C Human)
    Use
    Tags
    6xHis, GFP, and GST
    Expression
    Bacterial
    Mutation
    stalk region only amino acids 642 to 922
    Promoter
    T7
    Available since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pET28-His10-BTLAicd (AA190-289, Y243F, Y257F, Y282F)-TwinStrep

    Plasmid
    #180806
    Purpose
    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y243F, Y257F, Y282F)-TwinStrep
    Depositor
    Insert
    BTLAint (AA190-289, Y243F, Y257F, Y282F)-TwinStrep (BTLA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    BTLA (Y243F, Y257F, Y282F)
    Promoter
    T7
    Available since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pET28-His10-BTLAicd (AA190-289, Y226F, Y257F, Y282F)-TwinStrep

    Plasmid
    #180807
    Purpose
    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y257F, Y282F)-TwinStrep
    Depositor
    Insert
    BTLAint (AA190-289, Y226F, Y257F, Y282F)-TwinStrep (BTLA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    BTLA (Y226F, Y257F, Y282F)
    Promoter
    T7
    Available since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y282F)-TwinStrep

    Plasmid
    #180808
    Purpose
    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y282F)-TwinStrep
    Depositor
    Insert
    BTLAint (AA190-289, Y226F, Y243F, Y282F)-TwinStrep (BTLA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    BTLA (Y226F, Y243F, Y282F)
    Promoter
    T7
    Available since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y257F)-TwinStrep

    Plasmid
    #180809
    Purpose
    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y257F)-TwinStrep
    Depositor
    Insert
    BTLAint (AA190-289, Y226F, Y243F, Y257F)-TwinStrep (BTLA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    BTLA (Y226F, Y243F, Y257F)
    Promoter
    T7
    Available since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (wild type)

    Plasmid
    #153439
    Purpose
    E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Insert
    Rc.XDocB
    Use
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)

    Plasmid
    #153442
    Purpose
    E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.
    Depositor
    Insert
    Rc.XDocB
    Use
    Tags
    3xELP linker, 6xHis, Titin I27, ddFLN4 fingerprin…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 1861 - 1880 of 4188 results