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pAAV-DR1-NRSE-RFP

Plasmid

#22927

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

peroxisome proliferator activated receptor gamma binding site promoter with neuron-restrictive silencer element with minimal CMV

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-TorT(sp)

Plasmid

#111255

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorT signal peptide

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

TorT signal peptide

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pc

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p97-RG-mycStrep

Plasmid

#31840

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Use

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

R95G

Promoter

CMV

Available since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-hNPPC-RFP

Plasmid

#22921

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

human c-type natruiretic peptide precursor promoter driving DsRedExpress (NPPC Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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Pr2-deGFP-MGapt

Plasmid

#67736

Purpose

OR2-OR1-Pr2 promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T500

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

OR2-OR1-Pr2

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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Pr1-deGFP-MGapt

Plasmid

#67735

Purpose

OR2-OR1-Pr1 promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T500

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

OR2-OR1-Pr1

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAPi

Plasmid

#127548

Purpose

Empty control for pGAPi-based RNAi silencing in tandem with APT transcript segment

Depositor

Luis Vidali

Article

Orr et al Plant Physiol. 2020 Aug 6. pii: pp.20.00

Insert

Adenine Phosphorybosyltransferase transcript segment

Use

RNAi; Empty control

Tags

Expression

Mutation

Promoter

Ubiquitin

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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MyoUTipGAPi

Plasmid

#127549

Purpose

Positive RNAi control for pGAPi-based RNAi silencing in tandem with APT transcript segment

Depositor

Luis Vidali

Article

Orr et al Plant Physiol. 2020 Aug 6. pii: pp.20.00

Insert

Myosin XIa+b 5'UTR and Adenine Phosphorybosyltransferase transcript segment

Use

RNAi; Positive control

Tags

Expression

Mutation

Promoter

Ubiquitin

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgYAP-2

Plasmid

#121423

Purpose

sgYAP-2 sequence: GAGATGACTTCCTGAACAGTG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Stanley Qi, Ovijit Chaudhuri

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

sgYAP-2

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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hRad51(G151D)–Cas9(D10A)

Plasmid

#125565

Purpose

Expresses the Rad51(G151D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51(G151D)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAP2050-2

Plasmid

#105243

Purpose

mammalian GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

GFP11 with extra-sequence (GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAP2042-1

Plasmid

#105244

Purpose

mammalian eGFP with TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP with extra-sequence (TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker::eGFP)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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A3H HapI-Cas9n-UGI

Plasmid

#119140

Purpose

Base editor made from APOBEC3H Haplotype I

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3H Haplotype I-Cas9 nickase-UGI

Use

CRISPR

Tags

Expression

Mutation

None

Promoter

CMV

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_hU6miR-G13-L

Plasmid

#25758

Purpose

Entry vector with human U6 promoter driving mouse G alpha 13 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 miR-shRNA

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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hRad51(F86E)–Cas9(D10A)

Plasmid

#125564

Purpose

Expresses the Rad51(F86E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51(F86E)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFP122

Plasmid

#21669

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Tags

Expression

Mutation

The second lacZ sequence has been modified, the E…

Promoter

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pRY1004

Plasmid

#21788

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

Dig1-YFP (DIG1 Budding Yeast, A. victoria)

Use

Tags

YFP

Expression

Yeast

Mutation

contains 5' 1000bp UTR (Dig1 promoter) Di…

Promoter

Available since

Sept. 18, 2009

Availability

Academic Institutions and Nonprofits only

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FLAG-mPar6a WT

Plasmid

#86148

Purpose

FLAG tagged mouse Par6 wild type - Binds to aPKC, Par3, Lgl, Cdc42

Depositor

Senthil Muthuswamy

Article

Nolan et al Cancer Res. 2008 Oct 15;68(20):8201-9.

Insert

Par6 (Pard6a Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only
pEN_TmiR_G13-L_G12-E

Plasmid

#25763

Purpose

Entry vector with TRE promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 and 12 miR-shRNA

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-13b-1

Plasmid

#22762

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 13b-1 (mir-13b-1 Fly, primary transcript)

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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