We narrowed to 7,283 results for: Tet-on

1. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

   Plasmid

   #69546

   Purpose

   Tet-On system expressing MyoD-2A-GFP-2xNLS.

   Depositor

   Charles Gersbach

   Inserts

   eGFP
   Myod1 (Myod1 Mouse)
   

   Use

   Lentiviral

   Tags

   2x nuclear localization sequence and T2A "sk…

   Mutation

   Removed stop codon to fuse it to the 2A-eGFPnls i…

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1

   Plasmid

   #184398

   Purpose

   Donor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Renilla luciferase beta-globin-PTC39
   

   Use

   CRISPR; Donor plasmid for hdr

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   PTC in beta-globin at amino acid position 39

   Promoter

   Tet-On 3G

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR

   Plasmid

   #68347

   Purpose

   AAV plasmid expressing the FlpO recombinase. with empty gRNA

   Depositor

   Jannik Elverløv-Jakobsen

   Insert

   FlPO
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4-Zeo

   Plasmid

   #78353

   Purpose

   Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

   Depositor

   Michael Elowitz

   Article

   Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

   Insert

   H2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Synthetic, Human)
   

   Use

   Synthetic Biology

   Tags

   rTetR

   Expression

   Mammalian

   Promoter

   pEF alpha

   Available Since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-Zeo

   Plasmid

   #78352

   Purpose

   Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

   Depositor

   Michael Elowitz

   Article

   Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

   Insert

   H2B-mCherry-T2A-rTetR-KRAB
   

   Use

   Synthetic Biology

   Tags

   rTetR

   Expression

   Mammalian

   Promoter

   pEF alpha

   Available Since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRRL-pEF-H2B-mCherry-T2A-rTetR-Dest-SV40

   Plasmid

   #186968

   Purpose

   Lentiviral destination vector for cloning desired protein as rTetR fusion for chromatin regulation reporter (pEF-H2B-mCherry-T2A-rTetR, with SV40) for expression in mammalian cells.

   Depositor

   Brian Liau

   Article

   Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

   Insert

   pEF-H2B-mCherry-T2A-rTetR-Dest-SV40
   

   Use

   Lentiviral

   Tags

   rTetR fusion

   Expression

   Mammalian

   Promoter

   EF-1alpha promoter

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS99 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-3C-MCS

   Plasmid

   #199352

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-3C-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagBFP-P2A-ALFA-3C

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTS111 pHAGE2 CMVtetO2 MCS-3C-22xGS-Alfa-P2A-TagBFP

   Plasmid

   #199364

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-ALFA-P2A-BFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-ALFA-P2A-TagBFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTS109 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-GFP

   Plasmid

   #199362

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TEV-SUMOstar-EGFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN- Incenp-GFP

    Plasmid

    #108484

    Purpose

    inducible expression of vsv-CenpB-1-166-dCEN-INCENP-EGFP

    Depositor

    Susanne Lens

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    CenpB-dCEN-INCENP (INCENP Human)
    

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    CEN box replaced by CenpB 1-166

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBEST-p15a-pBAD-TetO1-UTR1-deGFP-ssrA-T500

    Plasmid

    #45788

    Depositor

    Richard Murray

    Insert

    deGFP
    

    Use

    Synthetic Biology

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Promoter

    pBAD-tetO1

    Available Since

    July 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRRL-pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40

    Plasmid

    #186967

    Purpose

    Lentiviral vector encoding DNMT3A recruiter for methylation reporter (pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40) for expression in mammalian cells.

    Depositor

    Brian Liau

    Article

    Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

    Insert

    DNMT3L (DNMT3L Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    rTetR fusion

    Expression

    Mammalian

    Promoter

    EF-1alpha promoter

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXTK-BB7 [Backbone_repABC-pMlb-KanR-TetR-PaqCI-Compatible_PartTypeBB]

    Plasmid

    #229393

    Purpose

    XanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_repABC-pMlb-KanR-TetR-PaqCI-Compatible_PartTypeBB and an sfGFP dropout

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag-Xbp1(S255A)

    Plasmid

    #231592

    Purpose

    Express circular RNA translation reporter with 2xALFATag-10xSunTag-Xbp1(S255A) pause site under H1 promoter

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    Tornado-2xALFATag-10xSunTag-Xbp1(S255A) pause sequence
    

    Expression

    Mammalian

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCyno-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid

    #212830

    Purpose

    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the cynomolgus genome.

    Depositor

    Wolfgang Enard

    Article

    Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

    Insert

    ZIM3-KRAB-dCas9-P2A-mCherry
    

    Tags

    HA-tag and ZIM3-KRAB

    Expression

    Bacterial and Mammalian

    Promoter

    CAG, TetOn

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGorilla-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid

    #212831

    Purpose

    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the gorilla genome.

    Depositor

    Wolfgang Enard

    Article

    Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

    Insert

    ZIM3-KRAB-dCas9-P2A-mCherry
    

    Tags

    HA-tag and ZIM3-KRAB

    Expression

    Bacterial and Mammalian

    Promoter

    CAG, TetOn

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFUW-tetO-mCh-Gata2-del-NLS(380-440)

    Plasmid

    #193070

    Purpose

    doxycycline-inducible expression of mouse Gata2 without the nuclear localisation signal (amino acids 380-440), in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

    Insert

    mCh-Gata2-del-NSL(380-440)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    minimal CMV

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG306-pTet07-synYFP[TTG/AGA]-4xArg[CGA]-nLuc

    Plasmid

    #199759

    Purpose

    Elongation reporter construct to quantify elongation duration of 4_CGA stalling

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    synYFP[TTG/AGA]-4xArg[CGA]-nLuc
    

    Expression

    Yeast

    Promoter

    TetO7

    Available Since

    May 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG306-pTet07-synYFP[TTG/AGA]-6xArg[CGA]-nLuc

    Plasmid

    #199760

    Purpose

    Elongation reporter construct to quantify elongation duration of 6_CGA stalling

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    synYFP[TTG/AGA]-6xArg[CGA]-nLuc
    

    Expression

    Yeast

    Promoter

    TetO7

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG306-pTet07-synYFP[TTG/AGA]-6xArg[AGA]-nLuc

    Plasmid

    #199761

    Purpose

    Elongation reporter construct to quantify elongation duration of 6_AGA stalling

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    synYFP[TTG/AGA]-6xArg[AGA]-cLuc
    

    Expression

    Yeast

    Promoter

    TetO7

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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