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pJW4.15

Plasmid

#62870

Purpose

Bacterial expression of MBP-PAA-1

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-PAA-1

Tags

MBP

Expression

Bacterial

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pJW4.23

Plasmid

#62876

Purpose

Bacterial expression of MBP-MEG-3

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MEG-3

Tags

MBP

Expression

Bacterial

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pJW4.21

Plasmid

#62875

Purpose

Bacterial expression of MBP-MEG-1

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MEG-1

Tags

MBP

Expression

Bacterial

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pNG2462

Plasmid

#37836

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Pnhx-2F13D12.6::YFP (F13D12.6 Nematode)

Tags

YFP

Expression

Worm

Promoter

nhx-2

Available Since

Dec. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pBSc-Shab6

Plasmid

#16099

Depositor

Larry Salkoff

Article

Wei et al Neuropharmacology. 1996 . 35(7):805-29.

Insert

Y55F3C.3 (Y55F3C.3 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pHCMM1

Plasmid

#167452

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

TEF1

Availability

Academic Institutions and Nonprofits only

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pHCMM2

Plasmid

#167453

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

PSEC63

Availability

Academic Institutions and Nonprofits only

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pHCMM3

Plasmid

#167454

Purpose

A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

PSEC63

Availability

Academic Institutions and Nonprofits only

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pAAP35

Plasmid

#250142

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(IDR-RGG) (pgl-3 Nematode)

Tags

MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pAAP36

Plasmid

#250143

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-IDR-RGG) (pgl-3 Nematode)

Tags

MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pAAP37

Plasmid

#250144

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D2-IDR-RGG) (pgl-3 Nematode)

Tags

MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK042

Plasmid

#250139

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-D2-IDR) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK082

Plasmid

#250140

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-D2-RGG) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pREA16

Plasmid

#245771

Purpose

Expresses mCherry::RBD::T2A::rac-2::mGFP in cholinergic motor neurons

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

mCherry::RBD::T2A::rac-2::mGFP

Tags

mCherry and mGFP

Expression

Worm

Promoter

unc-17b

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 N-MYC pdzd-8

Plasmid

#227844

Purpose

Used for transfection of cells for expression of C-MYC pdzd-8

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

pdzd-8 (C53B4.4 Nematode)

Tags

MYC

Expression

Mammalian

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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RT-5

Plasmid

#220442

Purpose

Construct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)

Depositor

Marvin Wickens

Article

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Insert

PUF3 (puf-3 Nematode)

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHIT353

Plasmid

#204506

Purpose

In vitro transcription of RNA probes for in situ hybridization of CeRep55 lncRNAs in nematode (after linearization with NotI and BglI)

Depositor

Yuji Kohara

Article

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

CeRep55 tandem repeats from Y73B3A (C. elegans)

Use

Other

Promoter

double-T7

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pHIT203

Plasmid

#204512

Purpose

Bacterial expression of C04F12.1 with 6xHis and maltose binding protein tags for in vitro assays

Depositor

Yuji Kohara

Article

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

C04F12.1 (C. elegans)

Tags

6xHis, MBP

Expression

Bacterial

Promoter

T7lac

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHIT239

Plasmid

#204522

Purpose

In vitro transcription of Mos1 transposase mRNA with SL1 and poly(A) used for microinjection into nematode

Depositor

Yuji Kohara

Article

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

Mos1_transposase::glh-2_3'UTR (glh-2 Nematode)

Use

Other

Promoter

T7 (minimum seq.)

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHIT517

Plasmid

#204518

Purpose

Bacterial expression of CEC-4 with 6xHis and maltose binding protein tags for in vitro assays

Depositor

Yuji Kohara

Article

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

cec-4 (C. elegans)

Tags

6xHis, MBP

Expression

Bacterial

Promoter

T7lac

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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