We narrowed to 148,791 results for: ins;

1. pAB082a

   Plasmid

   #79233

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ-Cry1Ac-d123
   

   Expression

   Bacterial

   Promoter

   Ppro1

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV_Stargazin-mTurquoise2-iLID

   Plasmid

   #161001

   Purpose

   Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusion

   Depositor

   Sean R Collins

   Article

   Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

   Insert

   Stargazin-mTurquoise2-iLID
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TBP6.7 non-targeting gRNA lambda phage

   Plasmid

   #132547

   Purpose

   expresses gRNA for TBP-based CIRTS

   Depositor

   Bryan Dickinson

   Article

   Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

   Insert

   gRNA TBP-based CIRTS
   

   Expression

   Mammalian

   Promoter

   hU6 promoter

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTF-lacZ

   Plasmid

   #153036

   Purpose

   For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-memRFP

   Plasmid

   #224564

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and membrane RFP reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   mRFP1
   

   Use

   Unspecified

   Tags

   Membrane localization signal

   Mutation

   Codon optimized

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMH-HA/Joseph2-Multitagged Plasmid

   Plasmid

   #226726

   Purpose

   Validates the expression and detection of epitope-tagged proteins of interest in mammalian cells.

   Depositor

   Richard Cripps

   Article

   Robinson et al Biotechniques. 2024;76(7):299-309. doi:

   Insert

   pMH-HA/Joseph2
   

   Tags

   HA, Myc, GFP, Flag, V5, His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 24, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAB061i

   Plasmid

   #79226

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ-HA4; PlacIq lacI
   

   Expression

   Bacterial

   Promoter

   Ppro1; PlacIq

   Available Since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSb_init containing the MBP sybody Sb_MBP#1

   Plasmid

   #132699

   Purpose

   expresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISA

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   sybody_MBP#1
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p6618 MSCV-IP N-HAonly WHSC2

   Plasmid

   #35014

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   WHSC2 (NELFA Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   MSCV LTR

   Available Since

   Feb. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

10. DT0

    Plasmid

    #80418

    Purpose

    Expresses 0 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAB061i3

    Plasmid

    #79230

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Ppro1 rpoZ; PlacIq lacI
    

    Expression

    Bacterial

    Promoter

    Ppro1; PlacIq

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS41K-mScarlet-I-mNeonGreen (pKBJ010)

    Plasmid

    #173461

    Purpose

    C-SWAT donor for Seamless tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-I-mNeonGreen
    

    Expression

    Yeast

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIVT-dCas9-mCherry

    Plasmid

    #174443

    Purpose

    in vitro transcription template for dCas9-mCherry

    Depositor

    Michael Lampson

    Article

    Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

    Insert

    Cas9 Endonuclease Dead
    

    Use

    In vitro transcription template

    Tags

    mCherry

    Promoter

    T7 SP6

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-EGFP-C1-HsTNRC6A

    Plasmid

    #25035

    Depositor

    Elisa Izaurralde

    Article

    Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIVT-dCas9-EGFP

    Plasmid

    #174442

    Purpose

    in vitro transcription template for dCas9-EGFP

    Depositor

    Michael Lampson

    Article

    Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

    Insert

    Cas9 Endonuclease Dead
    

    Use

    In vitro transcription template

    Tags

    EGFP

    Promoter

    T7 SP6

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV_ADRB2-mTurquoise2-iLID

    Plasmid

    #161002

    Purpose

    Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusion

    Depositor

    Sean R Collins

    Article

    Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

    Insert

    ADRB2-mTurquoise2-iLID
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDonor_RB-TnV2

    Plasmid

    #213907

    Purpose

    Barcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sites

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    mariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. RIP Cre-ER (DM#265)

    Plasmid

    #15029

    Depositor

    Douglas Melton

    Article

    Dor et al Nature. 2004 May 6. 429(6987):41-6.

    Insert

    Rat insulin promoter (RIP) (Ins2 Rat)
    

    Use

    Cre/Lox; For making transgenic constructs

    Tags

    Cre-ER (TM)

    Expression

    Mammalian

    Available Since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAB078d5

    Plasmid

    #79204

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) luxAB; Ppro1 434cI
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAB094a

    Plasmid

    #79241

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PBAD rpoZ-HA4; PC araC
    

    Expression

    Bacterial

    Promoter

    PBAD; PC

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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