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178,099 results

Showing: 9511 - 9520 of 178099 results

  3. pM-Cas9

    Plasmid
    #196325
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP
    Depositor
    Zhenghe Li
    Publication
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding SpCas9 in place of viral GP
    Use
    CRISPR
    Tags
    3×flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196325-plasmid-map-sequence-id-390015

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  6. lentiGuideFE-Puro

    Plasmid
    #170069
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter
    Depositor
    Neville Sanjana
    Publication
    Morris et al Science. 2023 May 19;380(6646):eadh7699.
    Insert
    Cas9 guide RNA scaffold with the F+E scaffold modification
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    170069-plasmid-map-sequence-id-333408

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  7. pcDNA3/er-(n2)oxStayGold(c4)

    Plasmid
    #185822
    Purpose
    A soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.
    Depositor
    Atsushi Miyawaki
    Publication
    Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    er-(n2)oxStayGold(c4)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    185822-plasmid-map-sequence-id-366124

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  8. SpySwitch

    Plasmid
    #184225
    Purpose
    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.
    Depositor
    Mark Howarth
    Publication
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpySwitch
    Tags
    His6
    Expression
    Bacterial
    Mutation
    E77A mutation prevents isopeptide bond formation,…
    Promoter
    T7
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    184225-plasmid-map-sequence-id-361604

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  10. GalT-RpHLuorin2

    Plasmid
    #171719
    Purpose
    Trans-Golgi expression of ratiometric pHLuorin2
    Depositor
    Geert van den Bogaart
    Publication
    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ratiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)
    Tags
    ratiometric pHluorin2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    171719-plasmid-map-sequence-id-355810

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Showing: 9511 - 9520 of 178099 results