We narrowed to 12,311 results for: BASE

1. β2AR-PPI-CALPon1.0 (TEVp)

   Plasmid

   #213407

   Purpose

   Peroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 2)

   Depositor

   Wenjing Wang

   Article

   Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

   Insert

   HA-arrestin-TEVp
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Ca-LiPPI-CLAPon1.0 (TEVp)

   Plasmid

   #213409

   Purpose

   Light-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 2)

   Depositor

   Wenjing Wang

   Article

   Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

   Insert

   CaM-V5-TEVp
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGG-TurboID-GFP

   Plasmid

   #209399

   Purpose

   Transiently expressing and visualizing the subcellular localization of TurboID in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   3XHA-TurboID
   

   Use

   Tags

   GFP

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCX:γB2ΔICD-EC1-G4S-mTQ2ΔC6

   Plasmid

   #209089

   Purpose

   mTurquoise2-inserted PcdhgB2 lacking an intracellular domain (ICD)

   Depositor

   Takeharu Nagai

   Article

   Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

   Insert

   PcdhgB2 (Pcdhgb2 Mouse)
   

   Use

   Tags

   PA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken β-actin

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCX:γB2ΔICD-EC5-VenusΔN3C9-P3

   Plasmid

   #209090

   Purpose

   mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

   Depositor

   Takeharu Nagai

   Article

   Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

   Insert

   PcdhgB2 (Pcdhgb2 Mouse)
   

   Use

   Tags

   PA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken β-actin

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. qTAG-C-sTagRFP-Zeo

   Plasmid

   #207734

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. qTAG-C-miRFP670nano3-Zeo

   Plasmid

   #207735

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. qTAG-N-Zeo-moxGFP

   Plasmid

   #207699

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-moxGFP cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. qTAG-N-Zeo-sTagRFP

   Plasmid

   #207701

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-sTagRFP cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. qTAG-N-Zeo-miRFP670nano3

    Plasmid

    #207702

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miRFP670nano3 cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. qTAG-C-sTagRFP-Blast

    Plasmid

    #207712

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. qTAG-N-Puro-sTagRFP

    Plasmid

    #207690

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-sTagRFP cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. qTAG-N-Blast-sTagRFP

    Plasmid

    #207679

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-sTagRFP cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pB7_9-ND51_v8_X1

    Plasmid

    #191511

    Purpose

    Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Left)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    v8 ZF-DdCBE 9-ND51 X1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pL6_3xG34-DCAF8L2_X1

    Plasmid

    #191518

    Purpose

    Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Right)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    ZF-DdCBE 3xG34-DCAF8L2 X1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pL7_3xG35-EMILIN2_X1

    Plasmid

    #191517

    Purpose

    Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Left)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    ZF-DdCBE 3xG35-EMILIN2 X1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pB6_R13-ND51_v8_X1

    Plasmid

    #191512

    Purpose

    Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Right)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    v8 ZF-DdCBE R13-ND51 X1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLTR-DuvSAF

    Plasmid

    #190914

    Purpose

    Pseudotyping of lentiviral vectors

    Depositor

    Jakob Reiser

    Article

    Federici et al Genet Vaccines Ther. 2009 Jan 13;7:1. do

    Insert

    Lyssavirus duvenhage antigenic glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HIV-1 LTR

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. bpNLS-DddIA

    Plasmid

    #187847

    Purpose

    expresses the bpNLS-linked DddIA in mammalian cells

    Depositor

    Chengqi Yi

    Article

    Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

    Insert

    bpNLS-DddIA-bpNLS-p2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p663-UBC-miniTurbo-V5-CSNK2A2_IDG-K

    Plasmid

    #189870

    Purpose

    Gateway destination clone of CSNK2A2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    CSNK2A2 (CSNK2A2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p663-UBC-miniTurbo-V5-DCLK3_IDG-K

    Plasmid

    #189891

    Purpose

    Gateway destination clone of DCLK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    DCLK3 (DCLK3 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p667-UBC-ADCK2-V5-miniTurbo_IDG-K

    Plasmid

    #189908

    Purpose

    Gateway destination clone of ADCK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    ADCK2 (ADCK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPpT4- HTA1prom-TIR1-FLAG

    Plasmid

    #189725

    Purpose

    Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    HTA1

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPpT4- TEF2prom-TIR1-FLAG

    Plasmid

    #189726

    Purpose

    Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    TEF2

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pM159

    Plasmid

    #173215

    Purpose

    Expresses dCas9-sfGFP10

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    dCas9-sfGFP10M2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTS2370-Tier1-PhCMV-TetR_F-type

    Plasmid

    #169573

    Purpose

    Tier-1 vector encoding PhCMV-driven tetracycline-dependent F-type-based transactiavtor (PhCMV-TetR-F-type-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled transactivator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CSM160

    Plasmid

    #105684

    Purpose

    Plasmid containing an RFP dropout used to construct randomized PAM library

    Depositor

    Chase Beisel

    Article

    Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

    Insert

    RFP Golden Gate dropout
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Bba_R0040 TetR Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMCL8

    Plasmid

    #176184

    Purpose

    gRNA cloning cassette with RPR1p-tetO promoter and terminator RPT1t, RPR1p-tetO-GFP-scRNA-RPR1t

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMCL8_28bp

    Plasmid

    #176185

    Purpose

    plasmid containing scaffold gRNA for Cas9 followed by a 28bp Csy4 recognition sequence, RPR1p-tetO-GFP-scRNA-28bpCsy4-RPR1t

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMCL9

    Plasmid

    #176186

    Purpose

    2 mu cloning vector for single or multiplexed gRNAs, SNR52p-sfGFP-scRNA-SUP4t-CYC1t

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pColE1-AlphaOmega

    Plasmid

    #165867

    Purpose

    Terminal pBlueScript derived high-copy GB Cloning vector with both Alpha and Omega Entry points requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBlastR-GMRWhite

    Plasmid

    #165885

    Purpose

    Blasticidin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Transgenesis Ready Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRMCE{PuroR-5FCS-GMRWhite}-Vasa-FC31

    Plasmid

    #165888

    Purpose

    Puromycin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    All-in-one FC31 RMCE Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRMCE{BlastR-5FCS-GMRWhite}-Vasa-FC31

    Plasmid

    #165889

    Purpose

    Blasticidin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    All-in-one FC31 RMCE Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGal4BD 1-147

    Plasmid

    #165819

    Purpose

    1-147 AA of GAL4 DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Gal4BD 1-147
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGAL4AD 768-881

    Plasmid

    #165820

    Purpose

    768-881 AA of GAL4 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    GAL4AD 768-881
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p663-UBC-miniTurbo-V5-LMTK2_IDG-K

    Plasmid

    #170580

    Purpose

    Gateway destination clone of LMTK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-LMTK2 (LMTK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pM80

    Plasmid

    #173173

    Purpose

    HA-dCas9

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p663-UBC-miniTurbo-V5-EEF2K_IDG-K

    Plasmid

    #170592

    Purpose

    Gateway destination clone of EEF2K (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-EEF2K (EEF2K Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    July 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLenti-TK-Firefly-cjCD69

    Plasmid

    #122279

    Purpose

    Lentiviral vector for stable expression of Firefly luciferase.

    Depositor

    Demián Cazalla

    Article

    Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

    Inserts

    Firefly luciferase
    cjCD69 3'UTR
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge