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We narrowed to 5,034 results for: plasmid dna

Showing: 3801 - 3840 of 5034 results

  2. pKAR1

    Plasmid
    #23104
    Purpose
    Allows expression of shRNA from U6 promoter and a rescue cDNA from a doxycycline (Tet) inducible promoter in the same plasmid
    Depositor
    Randy Poon
    Article
    Ma et al Nucleic Acids Res. 2007 . 35(4):e22.
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    TRE for cDNA; U6 for shRNA
    Available since
    May 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    23104-plasmid-map-sequence-id-10551

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  4. pET-6His-MBP-MpAgo

    Plasmid
    #79249
    Purpose
    This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression
    Depositor
    Jennifer Doudna
    Article
    Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p
    Insert
    Marinitoga piezophila Argonaute
    Use
    CRISPR
    Tags
    6XHis-tag + MBP + PreScission Cleavage Site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    79249-plasmid-map-sequence-id-151486

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  6. pPP537

    Plasmid
    #158803
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    CasPhi-2
    Use
    Tags
    2xSV40NLS-6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158803-plasmid-map-sequence-id-309579

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  8. pHBSTC159

    Plasmid
    #124353
    Purpose
    Plasmid expressing inactive NpuDnaBΔ283 mini-intein inserted into CcdA antitoxin
    Depositor
    Hideo Iwai
    Article
    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb
    Insert
    NpuDnaBΔ283 (C1G) mini-intein
    Use
    Tags
    CcdA-C (SFAD)
    Expression
    Bacterial
    Mutation
    Deletion of 283-residue endonuclease domain, C1G
    Promoter
    STC
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124353-plasmid-map-sequence-id-242353

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  12. NSP3C-Flag

    Plasmid
    #210349
    Purpose
    Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells
    Depositor
    Michele Pagano
    Article
    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1
    Insert
    C terminal of SARS-CoV-2 NSP3 (ORF1ab )
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    210349-plasmid-map-sequence-id-418173

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  15. pDEST47-ARL11

    Plasmid
    #67470
    Purpose
    Mammalian expression of hArl11 with stop codon (native expression)
    Depositor
    Richard Kahn
    Article
    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.
    Insert
    ARL11 (ARL11 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    bp 261 G to A; silent mutation; bp 442 T to C; aa…
    Promoter
    CMV
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pDEST47-ARL4D

    Plasmid
    #67468
    Purpose
    Mammalian expression of hArl4D with stop codon (native expression)
    Depositor
    Richard Kahn
    Article
    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.
    Insert
    ARL4D (ARL4D Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    bp 272 A to C; N91T mutation; bp 327 T to G; sil…
    Promoter
    CMV
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. pPP381

    Plasmid
    #158798
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-2
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    RuvC-inactivating mutation D394A
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158798-plasmid-map-sequence-id-309523

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  22. pCAT009

    Plasmid
    #171635
    Purpose
    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-B2M
    mTagBFP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171635-plasmid-map-sequence-id-336490

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  23. pCAT005

    Plasmid
    #171632
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-B2M
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a and U6
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171632-plasmid-map-sequence-id-336487

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  24. pCAT007

    Plasmid
    #171634
    Purpose
    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-TRAC
    mTagBFP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171634-plasmid-map-sequence-id-336493

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  25. pJRH623

    Plasmid
    #171633
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-non-targeting
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a and U6
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171633-plasmid-map-sequence-id-336492

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  26. pJRH056

    Plasmid
    #171637
    Purpose
    Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-tdTomato
    mTagBFP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171637-plasmid-map-sequence-id-354439

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  27. pCAT024

    Plasmid
    #171636
    Purpose
    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BFP
    mTagBFP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    171636-plasmid-map-sequence-id-337539

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  31. pCAT003

    Plasmid
    #171628
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-TRAC
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and EF1-a
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171628-plasmid-map-sequence-id-336485

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  32. pKAR1/Pur

    Plasmid
    #23105
    Purpose
    Allows expression of shRNA from U6 promoter and a rescue cDNA from a doxycycline (Tet) inducible promoter in the same plasmid. Contains puromycin resistance gene
    Depositor
    Randy Poon
    Article
    Ma et al Nucleic Acids Res. 2007 . 35(4):e22.
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    TRE for cDNA; U6 for shRNA
    Available since
    May 27, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 3801 - 3840 of 5034 results