Addgene

We narrowed to 7,106 results for: cas9 plasmid

Showing: 1901 - 1920 of 7106 results

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PX458_ZNF146_2

Plasmid

#72361

Purpose

Encodes gRNA for 3' target of human ZNF146 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Insert

ZNF146 (ZNF146 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pDonor_mU6

Plasmid

#69350

Purpose

sgRNA scaffold and mouse U6 promoter

Depositor

Andrea Ventura

Article

Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

Insert

mU6 promoter and sgRNA scaffold flanked by BbsI sites

Use

CRISPR

Tags

Expression

Mutation

Promoter

mU6 promoter

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-p15

Plasmid

#62656

Purpose

Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid

Depositor

Kristala Prather

Article

Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

Insert

exo, beta, gam, sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pRG2

Plasmid

#104174

Purpose

sgRNA expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAP973-1

Plasmid

#99496

Purpose

TEV::linker::meGFP:3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::meGFP::3XFlag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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LRG2.1-BFP

Plasmid

#120577

Purpose

Lentiviral plasmid to co-express a guide RNA and EBFP.

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pDonor_hU6

Plasmid

#69312

Purpose

sgRNA scaffold and human U6 promoter

Depositor

Andrea Ventura

Article

Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

Insert

hU6 promoter and sgRNA scaffold flanked by BbsI sites

Use

CRISPR

Tags

Expression

Mutation

Promoter

human U6 promoter

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLX-sgRNA-BfuAI-2k

Plasmid

#112915

Purpose

Empty sgRNA expression plasmid

Depositor

Ren-Jang Lin

Article

Kurata et al RNA. 2018 May 2. pii: rna.066282.118. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pDonor_sU6

Plasmid

#69351

Purpose

sgRNA scaffold and synthetic U6 promoter

Depositor

Andrea Ventura

Article

Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

Insert

sU6 promoter and sgRNA scaffold flanked by BbsI sites

Use

CRISPR

Tags

Expression

Mutation

Promoter

synthetic U6 promoter

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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LRG2.1-TagBFP2

Plasmid

#124773

Purpose

Lentiviral plasmid to co-express a guide RNA and TagBFP2

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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BB3nK_ext_AC

Plasmid

#108686

Purpose

Plasmid for assembly of donor DNA templates consisting of two expression units

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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BB3nK_ext_AD

Plasmid

#108687

Purpose

Plasmid for assembly of donor DNA templates consisting of three expression units

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAS_sfGFP150K

Plasmid

#193313

Purpose

pAS control plasmid for sfGFP expression

Depositor

Simon Elsaesser

Article

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Insert

super folder GFP

Use

Tags

Expression

Mammalian

Mutation

150K in sfGFP

Promoter

EF1

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pWD509

Plasmid

#73739

Purpose

Gateway compatible [1-2] Entry Vector encoding 2xNLS-FLP-D5

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

2xNLS-FLP-D5

Use

Tags

Expression

Worm

Mutation

G5D

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-Puro-ccdB

Plasmid

#82580

Purpose

A modified version of plasmid pX330 (#42230): Added are a ccdB stuffer and a Puromycin selection cassette

Depositor

Ivo Huijbers

Article

Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMLS291

Plasmid

#73724

Purpose

SapTrap mCherry + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

mCherry + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS252

Plasmid

#73720

Purpose

SapTrap GFP + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS286

Plasmid

#73723

Purpose

Saptrap tagRFP + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

tagRFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAP974-1

Plasmid

#99497

Purpose

TEV::linker::tagRFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::tagRFP::3XFlag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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U6>sgRNA(F+E)

Plasmid

#59986

Purpose

Empty vector for sgRNA cloning (F+E modified backbone)

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ciinte.U6

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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