We narrowed to 16,131 results for: igh

1. pDawn-sfGFP

   Plasmid

   #107741

   Purpose

   Expresses superfolder GFP under the control of a blue-light transcriptional regulator

   Depositor

   Ingmar Riedel-Kruse

   Article

   Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

   Insert

   superfolder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLambda

   Available since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113343

   Purpose

   Expresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   p300core-dCas9-VP64-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-Apex2-Venus-CAAX

   Plasmid

   #168511

   Purpose

   Expression of Apex2 fused to Venus-CAAX for targeting to plasma membrane. Useful for correlated light-eletron microscopy experiments in neurons.

   Depositor

   Franck Polleux

   Article

   Hirabayashi et al Sci Rep. 2018 Sep 27;8(1):14491. doi: 10

   Insert

   Apex2
   

   Use

   Tags

   Venus-CAAX

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbE5a-Opto-Cre-Vvd

   Plasmid

   #134404

   Purpose

   Light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

   Depositor

   Mary Dunlop

   Article

   Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

   Insert

   Opto-Cre-Vvd
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Jan. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. GI-Gal4 DBD

   Plasmid

   #42500

   Purpose

   GIGANTEA fused with Gal4 DNA binding domain (DBD), part of a light-inducible transcription factor

   Depositor

   Ricardo Dolmetsch

   Article

   Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

   Insert

   GIGANTEA fused with Gal4 DBD
   

   Use

   Tags

   Gal4 DNA binding domain (DBD)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-LOV-VP16

   Plasmid

   #42499

   Purpose

   LOV (N terminal domain of FKF1) fused to VP16, part of a light-inducible transcription factor

   Depositor

   Ricardo Dolmetsch

   Article

   Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

   Insert

   FKF1 (N-LOV, G128D mutant) fused with VP16
   

   Use

   Tags

   F-type VP16 activation domain and HA tag

   Expression

   Mammalian

   Mutation

   G128D 1-168aa

   Promoter

   CMV

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBLADE_ONLY_C

   Plasmid

   #168050

   Purpose

   Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

   Depositor

   Barbara Di Ventura

   Article

   Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

   Insert

   Blue light-inducible AraC dimers in Escherichia coli
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101**

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBLADE_ONLY_A

   Plasmid

   #168051

   Purpose

   Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

   Depositor

   Barbara Di Ventura

   Article

   Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

   Insert

   Blue light-inducible AraC dimers in Escherichia coli
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101**

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Nano-lantern/pcDNA3

   Plasmid

   #51970

   Purpose

   mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJBJZ_003

    Plasmid

    #155358

    Purpose

    Rifampicin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

    Depositor

    Elisabeth Raleigh

    Article

    Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pLIC-SM-Myc

    Plasmid

    #111189

    Purpose

    High affinity Myc tagging of weakly expressed genes (optimized for Toxoplasma gondii)

    Depositor

    Silvia Moreno

    Article

    Hortua Triana et al J Eukaryot Microbiol. 2018 Apr 19. doi:

    Type

    Empty backbone

    Use

    Toxoplasma gondii expression

    Tags

    Spaghetti Monster Myc Tag

    Expression

    Mutation

    Promoter

    Endogenous

    Available since

    Aug. 2, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pUB-MSP1D1

    Plasmid

    #161609

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    UD-MSP1D1
    

    Use

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1 ( Q138W, L181W, Y236W)

    Promoter

    T7

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBMN HAYFP-LOV24

    Plasmid

    #49570

    Purpose

    regulate protein stability with light

    Depositor

    Thomas Wandless

    Article

    Bonger et al ACS Chem Biol. 2014 Jan 17;9(1):111-5. d

    Insert

    YFP-LOV24
    

    Use

    Retroviral

    Tags

    HA and YFP

    Expression

    Mutation

    delete the last 3 aa from AsLOV2, with I532A muta…

    Promoter

    LTR

    Available since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSFFV_Gal4LOV[SK22]-VP64-IRES-mCherry (pLZA140)

    Plasmid

    #203907

    Purpose

    Encodes LightsOut (AsLOV2(408-543) inserted in Gal4 SK22) transcription factor in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    Gal4LOV(SK22)-VP64-IRES-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA FLAG TORC1 (1-634)

    Plasmid

    #25718

    Depositor

    Marc Montminy

    Article

    Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

    Insert

    TORC1 (1-634) (CRTC1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGal4AD-CIBN

    Plasmid

    #28246

    Purpose

    Expresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CIB1 (1-171aa)
    

    Use

    Tags

    Gal4AD

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSUM-Kan-MCS2-gfp

    Plasmid

    #109290

    Purpose

    Expresses high levels of gfp in mycobacteria

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Kanamycin

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p-mCherry-N1-ChloC

    Plasmid

    #85461

    Purpose

    Historical plasmid. For inhibition of neuronal activity, #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

    Depositor

    Peter Hegemann

    Article

    Wietek et al Science. 2014 Mar 27.

    Insert

    ChloC
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E90R, T159C

    Promoter

    CMV (+enhancer)

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Nano-lantern/pRSETB

    Plasmid

    #51969

    Purpose

    bacterial expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LAMP-CeNL_pcDNA3

    Plasmid

    #89514

    Purpose

    Cyan color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    CeNL
    

    Use

    Tags

    LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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