We narrowed to 4,947 results for: mcs

1. pX552-hSyn-DIO-GCaMP8f_Grin1TTT gRNA

   Plasmid

   #215282

   Purpose

   Expresses Grin1 Control gRNA in a Cre dependent GCaMP8f vector

   Depositor

   Bryan Copits

   Article

   Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

   Insert

   Grin1 control gRNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3_ERBB3-NTEV-tcs-GV-2xHA

   Plasmid

   #214611

   Purpose

   ERBB3 receptor for split TEV assay

   Depositor

   Michael Wehr

   Article

   Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

   Insert

   ERBB3-NTEV (ERBB3 Human)
   

   Tags

   TEV-Gal4-VP16-2xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJL1-MBP-DQNAT

   Plasmid

   #198215

   Purpose

   pJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag

   Depositor

   Michael Jewett

   Article

   Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

   Insert

   MBP_DQNAT
   

   Tags

   6x His Tag and DQNAT glycosylation tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJL1-PD-DQNAT

   Plasmid

   #198216

   Purpose

   pJL1 plasmid encoding H. influenzae protein D (PD) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag

   Depositor

   Michael Jewett

   Article

   Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

   Insert

   PD-DQNAT
   

   Tags

   6x His Tag and DQNAT glycosylation tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL1-sfGFP-MOORmut

   Plasmid

   #198213

   Purpose

   pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing a non-permissible minimum optimal O-linked recognition site (MOORmut) followed by a 6x-His tag

   Depositor

   Michael Jewett

   Article

   Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

   Insert

   sfGFP_MOORmut
   

   Tags

   6x His Tag and mutated (non-permissible) MOORmut …

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. HyWnt3-ΔRep-D3:Luc

   Plasmid

   #192990

   Purpose

   Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D3)

   Depositor

   Brigitte Galliot

   Article

   Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

   Insert

   Hydra Wnt3 promoter
   

   Expression

   Mammalian

   Mutation

   partial deletion of the Wnt3 repressor element-D3

   Available Since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMeCP2miRFP670 pc3942

   Plasmid

   #167568

   Purpose

   The miRFP670-gene is ligated c-terminally to the rat meCP2-gene.

   Depositor

   Cristina Cardoso

   Article

   Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

   Insert

   MeCp2 (Mecp2 Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. HyWnt3-ΔRep-D1:Luc

   Plasmid

   #192988

   Purpose

   Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D1)

   Depositor

   Brigitte Galliot

   Article

   Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

   Insert

   Hydra Wnt3 promoter
   

   Expression

   Mammalian

   Mutation

   partial deletion of the Wnt3 repressor element-D1

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. HyWnt3-ΔRep-D2:Luc

   Plasmid

   #192989

   Purpose

   Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D2)

   Depositor

   Brigitte Galliot

   Article

   Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

   Insert

   Hydra Wnt3 promoter
   

   Expression

   Mammalian

   Mutation

   partial deletion of the Wnt3 repressor element-D2

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. HyWnt3-ΔRep-D4:Luc

    Plasmid

    #192991

    Purpose

    Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D4)

    Depositor

    Brigitte Galliot

    Article

    Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

    Insert

    Hydra Wnt3 promoter
    

    Expression

    Mammalian

    Mutation

    partial deletion of the Wnt3 repressor element-D4

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pODN-mNG-CfANLN

    Plasmid

    #183837

    Purpose

    Repair template for the N-terminal tagging of anillin with mNeonGreen in canine cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    Canis familiaris ANLN homology arms with mNeonGreen-linker
    

    Use

    CRISPR; Donor template

    Tags

    mNeonGreen-linker

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459-HypaCas9-mR2-H2BC11_sgRNA

    Plasmid

    #183881

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    H2BC11 sgRNA spacer (H2BC11 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459-HypaCas9-mR2-MYH10_sgRNA

    Plasmid

    #183886

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    MYH10 sgRNA spacer (MYH10 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. EGFP-uTEV1-SQSfl

    Plasmid

    #171010

    Purpose

    HiLITR protease with full-length SQS targeting information (ER)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-SQS(FL)
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    pTRE-tight (TetOn)

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV5-ratERK2-I84A

    Plasmid

    #40815

    Depositor

    Natalie Ahn

    Article

    Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

    Insert

    MAPK1 (Mapk1 Rat)
    

    Tags

    His6

    Expression

    Mammalian

    Mutation

    I84A

    Promoter

    CMV

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

16. AIRAP 5: AIRAP(1-50).GST,V1.pcDNA3

    Plasmid

    #21795

    Depositor

    David Ron

    Article

    Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

    Insert

    AIRAP (Zfand2a Mouse)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    amino acids 1-50

    Available Since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 PARL-FLAG-CT S65A

    Plasmid

    #13618

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    PARL (PARL, PARL Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 65 to Alanine

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3 PARL-FLAG-CT T69A

    Plasmid

    #13619

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Thr 69 to Ala

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3 PARL-FLAG-CT S65D

    Plasmid

    #13621

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Ser 65 to Asp

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3 PARL-FLAG-CT T69D

    Plasmid

    #13622

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Thr 69 to Asp

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only