We narrowed to 23,976 results for: nes

1. pCMV-SpCas9(H840A)-T2A-EGFP

   Plasmid

   #221231

   Purpose

   Express nCas9 nickase (H840A) with GFP reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   SpCas9(H840A)-T2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H840A

   Promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PUC19_C_tag

   Plasmid

   #186659

   Purpose

   Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   C-terminal 3xFlag-3xHA Tag
   

   Use

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. tdTomato-KIF1A

   Plasmid

   #102850

   Purpose

   Overexpresses KIF1A and cell fill TdTomato

   Depositor

   Erik Dent

   Article

   McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10

   Insert

   KIF1A (Kif1a Rat)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTrc99S-ssDsbA-MBP-4xDQNAT

   Plasmid

   #128398

   Purpose

   Periplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Maltose binding protein
   

   Use

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Y342H- please see depositor comments

   Promoter

   trc

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBa.GFP-KIF5B

   Plasmid

   #134625

   Purpose

   Mammalian expression of mouse KIF5B 376-963

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF5B (Kif5b Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT with deletion of the 377 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Lyso-mCherry-Rheb(C181S)

   Plasmid

   #192438

   Purpose

   Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   Lyso-mCherry-Rheb(C181S) (RHEB Human)
   

   Use

   Tags

   Full-length LAMP1 and mCherry

   Expression

   Mammalian

   Mutation

   Rheb Cys 181 mutated to Ser; abolishes C-terminal…

   Promoter

   CMV

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-mCherry-Rheb(C181S)

   Plasmid

   #192437

   Purpose

   Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-Rheb(C181S) (RHEB Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Rheb Cys 181 mutated to Ser; abolishes C-terminal…

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJL1-SpCas9

   Plasmid

   #117051

   Purpose

   In vitro expression of S. pyogenes Cas9 from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

   Insert

   S. pyogenes Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmTurqoise KIF1A shRNA

   Plasmid

   #102849

   Purpose

   Generates shRNA specific to Rat KIF1A along with cell fill pmTurqoise

   Depositor

   Erik Dent

   Article

   McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10

   Insert

   KIF1A-shRNA (KIF1A Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1-RNA

   Available since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKRG3-mU6-PUb-hSpCas9

    Plasmid

    #162162

    Purpose

    Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    N/A

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMJ923

    Plasmid

    #78313

    Purpose

    Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli

    Depositor

    Martin Jinek

    Article

    Burger et al Development. 2016 Apr 29. pii: dev.13480

    Insert

    SpCas9
    

    Use

    Tags

    HA-2xNLS-mCherry-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    human codon-optimized gene sequence

    Promoter

    T7

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEYFP-N1-A2BR

    Plasmid

    #37202

    Depositor

    Robert Tarran

    Article

    Watson et al FASEB J. 2011 Sep;25(9):2996-3003. Epub

    Insert

    A2BR (ADORA2B Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAdSh.PGK.Cas9

    Plasmid

    #58253

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoter

    Depositor

    Manuel Goncalves

    Article

    Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

    Insert

    Human codon-optimized S. pyogenes Cas9
    

    Use

    Adenoviral and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. L309-myc-seipin-WT

    Plasmid

    #64061

    Purpose

    myc-tagged murine seipin (wild type); lentiviral construct

    Depositor

    Weiping Han

    Article

    Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

    Insert

    bscl2 (Bscl2 Mouse)
    

    Use

    Lentiviral

    Tags

    myc epitope

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pTrc99S-ssDsbA-CRM197-4xDQNAT

    Plasmid

    #128403

    Purpose

    Periplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Diphtheria toxin
    

    Use

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Inactivating G52E mutation

    Promoter

    trc

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR

    Plasmid

    #162335

    Purpose

    Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    S. pyogenes sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. 3xFLAG-dCas9/MSCV-EGFP

    Plasmid

    #82613

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g

    Insert

    3xFLAG-dCas9
    

    Use

    Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-2)

    Plasmid

    #159660

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-3)

    Plasmid

    #159661

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-1)

    Plasmid

    #159659

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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