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pBbS1c-RFP

Plasmid

#35338

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Trc

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pICXC3GH

Plasmid

#49023

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Promoter

AOX1

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCD227

Plasmid

#113318

Purpose

AraC-pBAD-dCas9, MCP-SoxS_R93A

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Expression

Bacterial

Mutation

R93A

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pTORGm43GWC

Plasmid

#112903

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mTFP1.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTORGm43GWF

Plasmid

#112904

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mWasabi.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSBDEST.Z

Plasmid

#79470

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Zeocin

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBad33_ISEc41_reRNA

Plasmid

#205341

Purpose

Bacterial expression of ISEc41 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISEc41 TnpB

Expression

Bacterial

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBad33_ISEc46_reRNA

Plasmid

#205343

Purpose

Bacterial expression of ISEc46 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISEc46 TnpB

Expression

Bacterial

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBad33_ISNsp2_reRNA

Plasmid

#205350

Purpose

Bacterial expression of ISNsp2 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISNsp2 TnpB

Expression

Bacterial

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pACYC.Taq.1Amb

Plasmid

#102794

Purpose

Expresses Taq DNA polymerases with a premature stop codon

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Taq DNA polymerase

Expression

Bacterial

Mutation

W168Stop; *(see below)

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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