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2,384 results

Showing: 1921 - 1940 of 2384 results

  2. pTK41

    Plasmid
    #236110
    Purpose
    Expresses the motor domain of rat KIF5C fused with SNAP tag and ALFA tag nanobody
    Depositor
    Shinsuke Niwa
    Publication
    Kita et al Cell Rep Phys Sci, 6 (2): 102440. 19 Feb
    Inserts
    KIF5C(C7S)(1-430)-SNAP-His (Kif5c Rat)
    KIF5C(C7S)(1-430)-ALFA tag nanobody-StrepII tag (Kif5c Rat)
    Tags
    ALFA tag nanobody, His tag, SNAP tag, and StrepII…
    Expression
    Bacterial
    Mutation
    C7S mutation is introduced. 1-430 aa is encoded.
    Promoter
    T7
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236110-plasmid-map-sequence-id-472830

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  4. pMRX-IP-EGFP-LOV2-OMP25

    Plasmid
    #233346
    Purpose
    Stable expression of EGFP-LOV2(N538E)-OMP25 in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.
    Depositor
    Noboru Mizushima
    Publication
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    Omp25 (Synj2bp Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N538E and N-terminus is deleted
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233346-plasmid-map-sequence-id-469428

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  12. pET28-NFHΔ36-72

    Plasmid
    #232060
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72
    Depositor
    Sanjay Kumar
    Publication
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Heavy tail domain lacking residues 36-72
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    added N-terminal tryptophan and tyrosine, and cys…
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232060-plasmid-map-sequence-id-464069

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  13. pET28-NFHΔ462-638

    Plasmid
    #232061
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638
    Depositor
    Sanjay Kumar
    Publication
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Heavy tail domain lacking residues 462-638
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    added N-terminal tryptophan and tyrosine, and cys…
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232061-plasmid-map-sequence-id-464068

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  14. pET28-NFLtail

    Plasmid
    #232055
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFL tail domain
    Depositor
    Sanjay Kumar
    Publication
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Light tail domain
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    cDNA codon-optimized for E. coli; added N-termina…
    Promoter
    T7
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232055-plasmid-map-sequence-id-464065

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  15. pET28-NFMtail

    Plasmid
    #232056
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFM tail domain
    Depositor
    Sanjay Kumar
    Publication
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Medium tail domain
    Tags
    6xHis, thrombin
    Expression
    Bacterial
    Mutation
    cDNA codon-optimized for E. coli; added N-termina…
    Promoter
    T7
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232056-plasmid-map-sequence-id-464064

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  16. pET28-NFMshuf1

    Plasmid
    #232058
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 1
    Depositor
    Sanjay Kumar
    Publication
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Medium tail domain, charge shuffle 1
    Tags
    6xHis, thrombin
    Expression
    Bacterial
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232058-plasmid-map-sequence-id-464062

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  17. pET28-NFHtail

    Plasmid
    #232057
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFH tail domain
    Depositor
    Sanjay Kumar
    Publication
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Heavy tail domain
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    added N-terminal tryptophan and tyrosine, and cys…
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232057-plasmid-map-sequence-id-464061

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  18. pET28-NFMshuf2

    Plasmid
    #232059
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 2
    Depositor
    Sanjay Kumar
    Publication
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Medium tail domain, charge shuffle 2
    Tags
    6xHis, thrombin
    Expression
    Bacterial
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232059-plasmid-map-sequence-id-464059

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  20. pdnCas3-APOBEC1

    Plasmid
    #229537
    Purpose
    pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Publication
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    rAPOBEC1
    dnCas3
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229537-plasmid-map-sequence-id-459005

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Showing: 1921 - 1940 of 2384 results