We narrowed to 4,447 results for: Alk

Showing: 1921 - 1940 of 4447 results

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Met133ILeu, Met186ILeu ELL2

Plasmid

#127264

Purpose

For in vitro translation of human ELL2 with Met133ILeu, Met186ILeu

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Two Mets (133,186 )to ILeu Quikchange

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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Met331Leu ELL2

Plasmid

#127262

Purpose

For in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met331 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met331 to ILeu Quikchange

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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COOHend ELL2

Plasmid

#127255

Purpose

Expresses the C terminal segment of human ELL2 in mammalian cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (C-terminal end) (ELL2 Human)

Use

Other

Expression

Mammalian

Mutation

contains only the last 389 aa

Promoter

pEF1a

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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Met1ILeu ELL2

Plasmid

#127258

Purpose

For in vitro translation of human ELL2 with Met1 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met1 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met1 to Ileu by Quikchange

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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ELL2mRNA+polyA

Plasmid

#127256

Purpose

For in vitro translation of human ELL2 with 30 nt pA tail

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

shortens 3'UTR to 40 bp adds A30 tail

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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NH2end ELL2

Plasmid

#127252

Purpose

Expresses the N terminal segment of human ELL2 in mammalian cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (1-287) (ELL2 Human)

Use

Other

Tags

His6X

Expression

Mammalian

Mutation

contains only amino acids 1-289

Promoter

pEF1a

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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HA tagged Xba ELL2

Plasmid

#127268

Purpose

For in vitro translation of WT ELL2 with HA tag inserted near N-term

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

ELL2mRNA+polyA with HA tag inserted at aa12 betwe…

Promoter

T7

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only
pBABEpuro-gateway

Plasmid

#51070

Purpose

gateway adapted mammalian expression vector

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gα12-133-NLuc

Plasmid

#207156

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNA12 (GNA12 Human)

Expression

Mammalian

Mutation

NLuc inserted at position A(133)F

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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8xNFAT-ZsG-hCD8

Plasmid

#153417

Purpose

NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )

Use

Retroviral

Expression

Mammalian

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2_1-180_AE

Plasmid

#148578

Purpose

Mammalian Expression of HsGIGYF2_1-180

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_1-180 (GIGYF2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NPM1

Plasmid

#237667

Purpose

For overexpression of mEGFP-NPM1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1 (NPM1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-Exo70

Plasmid

#53761

Purpose

pEGFP-C3 plasmid that expresses Exo70

Depositor

Channing Der

Article

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Exo70 (EXOC7 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pX028

Plasmid

#167151

Purpose

MoClo-compatible Level 1 (position 2) vector encoding Neonothopanus nambi hispidin synthase nnHispS under control of 0.4 kb 35S promoter, for expression in plants

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

p35s-nnHispS-ocsT

Expression

Plant

Promoter

p35s

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX029

Plasmid

#167152

Purpose

MoClo-compatible Level 1 (position 3) vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H under control of 0.4 kb 35S promoter, for expression in plants

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

p35s-nnH3H-ocsT

Expression

Plant

Promoter

p35s

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-Sec8

Plasmid

#53758

Purpose

pEGFP-C3 plasmid that expresses Sec8

Depositor

Channing Der

Article

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Sec8 (EXOC4 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pNK5785

Plasmid

#219753

Purpose

pGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - nnLuz_v4 - tAOX

Use

Luciferase

Expression

Yeast

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-Lepr_FL-IRES-eGFP

Plasmid

#196635

Purpose

Doxycycline inducible expression of full length mouse Lepr with eGFP marker

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

Lepr (Lepr Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pNK5664

Plasmid

#219747

Purpose

DVK_AF CIDAR vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of CMV promoter, for mammalian expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pCMV - nnLuz_v4 - tSV40

Use

Luciferase

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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R619-M66-303: CMV51p> HCoV-OC43 S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166015

Purpose

mammalian expression of HCoV-OC43 soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

HCoV-OC43 S (spike)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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