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AAV_A2S-8

Plasmid

#188654

Purpose

AAV vector expressing SaAID-2S and gRNA

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Inserts

AID2S--nSaCas9-UGI
Sa-sgRNA

Use

AAV and CRISPR

Tags

UGI

Expression

Mammalian

Mutation

D10A for SaCas9

Promoter

Scp1 and U6

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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U_(+36GFP)-Cre.ape

Plasmid

#71749

Purpose

U+36GFP-Cre fusion expression in E. Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_(pos36GFP)-Cre

Tags

6XHis

Expression

Bacterial

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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Aurein1.2_(+36GFP)-BirA

Plasmid

#71752

Purpose

Aurein1.2+36GFP-BirA fusion for E.Coli expression

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

Aurein1.2+36GFP-BirA

Tags

6XHis

Expression

Bacterial

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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SEPT11 sh1 3m shRNA

Plasmid

#122021

Purpose

Negative control shRNA for SEPT11 sh1 shRNA (plasmid 122020).

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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Septin 11 isoform II

Plasmid

#120315

Purpose

Septin 11 isoform II mammalian expression

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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PABE-5

Plasmid

#115626

Purpose

Targeted A to G in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

NLS-nCas9-NLS-wtTadA-TadA7.10-NLS

Use

CRISPR

Expression

Plant

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-P85A

Plasmid

#33062

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

P85A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
His-TEV-U_(+36GFP)-Cre

Plasmid

#71750

Purpose

His-TEV-U+36GFP-Cre for E.Coli expression

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_(pos36GFP)-Cre

Tags

6X His and TEV protease

Expression

Bacterial

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA-H1-sgRNA-hTZAP

Plasmid

#87186

Purpose

guideRNA targeting exon1 of human TZAP

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

TZAP (ZBTB48 Human)

Use

CRISPR

Expression

Mammalian

Promoter

H1

Available Since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-TZAPZNF9-11

Plasmid

#87184

Purpose

retroviral stable expression of truncation protein Flag-TZAPZNF9-11

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

TZAP (ZBTB48 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pdFLPtag-SA-SD-0-GFSTF

Plasmid

#247949

Purpose

Conditional tagging of endogenous proteins with GFSTF in open reading frame 0, with dual membrane labelling

Depositor

Sha Liu

Article

Li et al Sci Rep. 2025 Nov 27;15(1):45542. doi: 1

Insert

GFSTF

Tags

GFSTF

Expression

Insect

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pdFLPtag-SA-SD-1-GFSTF

Plasmid

#247950

Purpose

Conditional tagging of endogenous proteins with GFSTF in open reading frame 1, with dual membrane labelling

Depositor

Sha Liu

Article

Li et al Sci Rep. 2025 Nov 27;15(1):45542. doi: 1

Insert

GFSTF

Tags

GFSTF

Expression

Insect

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pdFLPtag-SA-SD-2-GFSTF

Plasmid

#247951

Purpose

Conditional tagging of endogenous proteins with GFSTF in open reading frame 2, with dual membrane labelling

Depositor

Sha Liu

Article

Li et al Sci Rep. 2025 Nov 27;15(1):45542. doi: 1

Insert

GFSTF

Tags

GFSTF

Expression

Insect

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pSYL018

Plasmid

#246594

Purpose

template plasmid for site-saturation mutagenesis library cloning

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1 variant 2b (AT3G04550 Mustard Weed)

Expression

Bacterial

Mutation

AtRaf1I90V/I127F/R139H/M243K/E275V/V334A/K411I/R4…

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pSYL017

Plasmid

#246593

Purpose

template plasmid for site-saturation mutagenesis library cloning

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1 variant 2g (AT3G04550 Mustard Weed)

Expression

Bacterial

Mutation

AtRaf1L121F/I127L/A195V/N351Y/R432G

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_21 tRNA genes

Plasmid

#242430

Purpose

In vitro transcription of 21 tRNAs after Nt.BspQI treatment. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene.

Depositor

Sebastian Maerkl

Article

Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:

Insert

21 tRNA genes with a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene

Expression

Bacterial

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-RUSH-Strep-KDEL

Plasmid

#228527

Purpose

Lentiviral vector, cloning vector for Strep-KDEL-IRES in mammalian cells.

Depositor

Ginny Farias

Article

Li et al Nat Commun. 2024 Dec 30;15(1):10829. doi

Insert

Strep-KDEL

Use

Lentiviral

Tags

Streptavidin-KDEL

Expression

Mammalian

Promoter

Ubc

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLPP4

Plasmid

#209966

Purpose

Contains Level 0 Part: constitutive Promoter (P40) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P40)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLP-EV

Plasmid

#209973

Purpose

Contains Level 0 Part: mock coding sequence (EV) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

CDS (mock)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_zCas9

Plasmid

#213913

Purpose

Entry clone containing 3×FLAG-NLS-zCas9-NLS encoding sequence, Pisum sativum rbcS E9 terminator. For use in plants and compatible with the MultiSite Gateway system

Depositor

Sébastien Mongrand

Article

Li et al Bio Protoc. 2024 Jul 5;14(13):e5029. doi

Insert

zCas9

Use

CRISPR

Mutation

Zea mays codon-optimized Cas9

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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