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pYNL-C1_RhoB

Plasmid

#65696

Purpose

Expresses YNL-RhoB in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

RhoB (RHOB Human)

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pONL-N1_vinculin

Plasmid

#65692

Purpose

Expresses Vinculin-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

vinculin (Vcl Mouse)

Use

Luciferase

Tags

ONL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pYNL-N1_vinculin

Plasmid

#65690

Purpose

Expresses Vinculin-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

vinculin (Vcl Mouse)

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pClneoMyc'-cYAP-1

Plasmid

#66854

Purpose

Express Myc-tagged C.elegans YAP-1 in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

YAP-1 (yap-1 Nematode)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pClneoMyc'-EGL-44

Plasmid

#66855

Purpose

Express Myc-tagged C.elegans EGL-44 in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

EGL-44 (CELE_F28B12.2 Nematode)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pClneoEGFPC-let60 K16N

Plasmid

#66861

Purpose

Express GFP-tagged dominat negative let60, in which Lysine 16 is mutated to asparagine, in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Insert

Let-60 (let-60 Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

Lysine 16 is mutated to asparagine

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pONL-N1_ITPKA

Plasmid

#65689

Purpose

Expresses ITPKA-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

inositol triphosphate 3-kinase A

Use

Luciferase

Tags

ONL

Expression

Mammalian

Mutation

contains residues 9-52

Promoter

CMV

Available since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

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pYNL-C1_PTS1

Plasmid

#65699

Purpose

Expresses YNL-PTS1 in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 1 (tripeptide SKL)

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCNL-N1_Golgi

Plasmid

#65694

Purpose

Expresses Golgi-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

N-terminal 81 amino acids of beta 1,4-galactosyltransferase

Use

Luciferase

Tags

CNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCNL-N1_TUBB5

Plasmid

#65679

Purpose

Expresses beta-tubulin-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

beta-tubulin class V

Use

Luciferase

Tags

CNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only
pYNL-N1_Golgi

Plasmid

#65693

Purpose

Expresses Golgi-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

N-terminal 81 amino acids of beta 1,4-galactosyltransferase

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYNL-N1_ITPKA

Plasmid

#65687

Purpose

Expresses ITPKA-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

inositol triphosphate 3-kinase A

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

contains residues 9-52

Promoter

CMV

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCNL-C1_PTS1

Plasmid

#65700

Purpose

Expresses CNL-PTS1 in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 1 (tripeptide SKL)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pONL-C1_PTS1

Plasmid

#65701

Purpose

Expresses ONL-PTS1 in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 1 (tripeptide SKL)

Use

Luciferase

Tags

ONL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pONL-N1_Mito

Plasmid

#65671

Purpose

Expresses Mito-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

cytochrome c oxidase subunit VIII

Use

Luciferase

Tags

ONL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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Neurl1Rm-Flag pBacPAK8-3

Plasmid

#17326

Depositor

Ronald Conaway, Joan Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Neuralized-like (Neurl1a Mouse)

Use

Tags

FLAG

Expression

Insect

Mutation

Cys 521 changed to Ser Cys 524 changed to Ser

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pAAV mGAD65(delE1) GFP WPRE SV40pA

Plasmid

#177316

Purpose

To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.

Depositor

Hirokazu Hirai

Article

Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10

Insert

mGAD65(delE1) promoter, GABAergic interneurons specific promoter

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
RGS-6xHis-BLIMP-1-pcDNA3.1-

Plasmid

#52518

Purpose

expresses human BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Blimp1 (PRDM1 Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Talin-TS

Plasmid

#83376

Purpose

FRET probe for measuring tension on Talin molecule

Depositor

Martin Schwartz

Article

Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

Insert

Talin1 (Tln1 Mouse)

Use

Retroviral

Tags

EGFP-F40-tagRFP (tension sensor module)

Expression

Mammalian

Mutation

Tension sensor module is inserted between V447 an…

Promoter

CMV

Available since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Talin-CS

Plasmid

#83377

Purpose

Control FRET probe for measuring tension on Talin molecule

Depositor

Martin Schwartz

Article

Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

Insert

Talin1 (Tln1 Mouse)

Use

Retroviral

Tags

EGFP-F40-tagRFP (tension sensor module)

Expression

Mammalian

Mutation

Tension sensor module is inserted at C-terminal o…

Promoter

CMV

Available since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge