We narrowed to 26,595 results for: STI
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Plasmid#103297PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-190a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-190a-3p target (MIR190A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-1307-5p
Plasmid#103215PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-1307-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-1307-5p target (MIR1307 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-1287-3p
Plasmid#103203PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-1287-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-1287-3p target (MIR1287 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-1287-5p
Plasmid#103204PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-1287-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-1287-5p target (MIR1287 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-1185-1-3p
Plasmid#103180PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-1185-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-1185-1-3p target (MIR1185-1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-1185-2-3p
Plasmid#103181PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-1185-2-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-1185-2-3p target (MIR1185-2 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-10a-5p
Plasmid#103177PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-10a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-10a-5p target (MIR10A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-10b-3p
Plasmid#103178PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-10b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-10b-3p target (MIR10B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-10b-5p
Plasmid#103179PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-10b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-10b-5p target (MIR10B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-lox-ERAS
Plasmid#52417PurposeDoxycycline inducible lentiviral vector of human ERAS cDNA with excisable insertDepositorAvailable SinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-2dV-Camui (wt)
Plasmid#220366PurposeImproved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)DepositorInsertmVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Synthetic, Rat)
UseTagsmEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)ExpressionMammalianMutationPromoterCAGAvailable SinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-ERK2-L4A-MEK1_fusion
Plasmid#39197DepositorUseTagsMycExpressionMammalianMutationL4A = L33A, L37A, L40A, L42APromoterCMVAvailable SinceJuly 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
CA-RIT-NFAT1 (IRES-GFP)
Plasmid#85181PurposeConstitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interaction. Co-expresses EGFP for selectionDepositorInsertCA-RIT-NFAT1 (Nfatc2 Mouse)
UseRetroviralTagsHAExpressionMammalianMutationAmino acids 1-3 removed; CA: PASSGSSASF mutated t…PromoterAvailable SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLIK CA MLCK
Plasmid#84647PurposeLentiviral expression of doxycycline-inducible constitutively active MLCK and co-expression of VenusDepositorInsertMLCK (MYLK Human)
UseLentiviralTagsExpressionMammalianMutationConstitutive ActivePromoterAvailable SinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)
Plasmid#114078PurposeCAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)DepositorInserthuman codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain
UseTagsSV40 NLSExpressionMammalianMutationPromoterAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCJ202
Plasmid#162675PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.DepositorInsertPETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
UseTagsHisExpressionBacterialMutationW159C, S238C; codon optimized for expression in E…PromoterT7/lacAvailable SinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
CA-RIT-NFAT1 (PGK-Puro)
Plasmid#63214PurposeConstitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interactio. Contains Puromycin selection markerDepositorInsertCA-RIT-NFAT1 (Nfatc2 Mouse)
UseRetroviralTagsHAExpressionMammalianMutationAmino acids 1-3 removed; CA: PASSGSSASF mutated t…PromoterAvailable SinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T2-Ste7-EE
Plasmid#52681Purposeexpression of constitutively active Ste7 kinase in bacteriaDepositorInsertSte7 (STE7 Budding Yeast)
UseTagsGST and MycExpressionBacterialMutationS359E/T363E constitutively activePromotertacAvailable SinceApril 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTF-ACE2s
Plasmid#162786PurposeThe extracellular domain of Angiotensin converting enzyme 2 (ACE2) expressionDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTags10xHis, FLAG, and hTPA leaderExpressionMammalianMutationPromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)
Plasmid#114090PurposeCAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tagDepositorInserthuman codon optimized MbCas12a
UseTagsNLS(nucleoplasmin) and 3xHAExpressionMammalianMutationPromoterAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only