We narrowed to 4,090 results for: PRS

1. pRS406 ATG9-GFP S122D

   Plasmid

   #114667

   Purpose

   Expresses yeast ATG9 (S122D) under the endogenous promoter with a GFP C-terminal tag

   Depositor

   Daniel Klionsky

   Article

   Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

   Insert

   ATG9
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   changed serine 122 to aspartic acid

   Promoter

   ATG9

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS426-TUB1-internal6xHis (1-440)

   Plasmid

   #60391

   Purpose

   Expression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoter

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB1 (TUB1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   internal 6xHis (see supplement figure 4), amino a…

   Promoter

   GAL

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pRS303-pGAL-HA-SrtAstrep (pKS78)

   Plasmid

   #50029

   Purpose

   Galactose-inducible expression of cytosolic S. pyogenes SrtA in yeast

   Depositor

   Hidde Ploegh

   Article

   Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

   Insert

   HA-SrtA(strep)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Promoter

   pGAL

   Available since

   Jan. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSF-KpDcr1deltaC(E224Q, GiVL-1)

   Plasmid

   #32033

   Depositor

   David Bartel

   Article

   Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

   Insert

   DCR1(15-355)
   

   Use

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   E224Q and H123-N143 replaced by P642-Y645 of GiDi…

   Promoter

   T7

   Available since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET FLIPE intramol-10u

   Plasmid

   #13550

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

   Insert

   FLIP-E intramol A207R (gltI E. coli)
   

   Use

   Tags

   EYFP

   Expression

   Bacterial

   Mutation

   A207R, ECFP insert in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sc FLAG-S6-RFC1-pRS405/GAL-L

   Plasmid

   #239473

   Purpose

   Overexpress Sc FLAG-S6-RFC1 in yeast (integrated)

   Depositor

   Michael O'Donnell

   Insert

   Sc FLAG-S6-RFC1 (RFC1 Budding Yeast)
   

   Use

   Tags

   3X FLAG followed by S6

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sc FLAG-RFC1-pRS405/GAL-L

   Plasmid

   #239476

   Purpose

   Overexpress Sc FLAG-RFC1 in yeast (integrated)

   Depositor

   Michael O'Donnell

   Insert

   Sc FLAG-RFC1 (RFC1 Budding Yeast)
   

   Use

   Tags

   3X FLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Sc [Pol31 + Pol32]-pRS403/GAL

   Plasmid

   #239199

   Purpose

   Overerxpress Sc Pol31 & Pol32 when integrated into yeast (S. cer)

   Depositor

   Michael O'Donnell

   Inserts

   Pol31 (POL31 Budding Yeast)
   Pol32 (POL32 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Sc Pol3-FLAG(Ec)-pRSF405/GAL

   Plasmid

   #239198

   Purpose

   Overexpress Sc Pol3(C-terminal 3X FLAG tag) when integrated into yeast (S. cer)

   Depositor

   Michael O'Donnell

   Insert

   Pol3 (POL3 Budding Yeast)
   

   Use

   Tags

   3X FLAG

   Expression

   Yeast

   Mutation

   E. coli codon bias (synthetic gene), but same ami…

   Promoter

   Available since

   June 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ec FkP-dnaN(3E's)-pRSFDuet

    Plasmid

    #238344

    Purpose

    over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coli

    Depositor

    Michael O'Donnell

    Insert

    PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96E; R103E; R105E) (dnaN E. coli)
    

    Use

    Tags

    3X FLAG+PKA kinase motif+PreScission Protease cle…

    Expression

    Bacterial

    Mutation

    R96E; R103E; R105E

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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