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pET-29_GSS-6xHis-SUMO-VidC-EGFP-FLAG

Plasmid

#241009

Purpose

VidC-EGFP construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-EGFP-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-29_GSS-6xHis-SUMO-VidC-mCherry-FLAG

Plasmid

#241010

Purpose

VidC-mCherry construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-mCherry-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-21_GSS-6xHis-SUMO-VidC-bla-FLAG

Plasmid

#241012

Purpose

VidC-bla construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-bla-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-21_GSS-6xHis-SUMO-VidC-EGF-FLAG

Plasmid

#241011

Purpose

VidC-EGF construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-EGF-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-443_Cacnes_HisB

Plasmid

#225608

Purpose

Suicide C. acnes vector to generate HisB (PPA_RS05815) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pC2.4

Plasmid

#226557

Purpose

NATURA gene encoding plasmid

Depositor

Dave Wee

Article

Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

Insert

NATURA reporter gene

Use

Luciferase; Piggybac

Tags

EGFP and Flag

Expression

Mammalian

Promoter

EF1alpha

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4_EGR1p-BC1484-luc2

Plasmid

#227138

Purpose

Barcoded assay, MAPK sensor; barcode BC1484

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

EGR1 promoter driving barcode 1484 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_EGR1p-BC0136-luc2

Plasmid

#227137

Purpose

Barcoded assay, MAPK sensor; barcode BC0136

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

EGR1 promoter driving barcode 0136 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_EGR1p-BC0132-luc2

Plasmid

#227136

Purpose

Barcoded assay, MAPK sensor; barcode BC0132

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

EGR1 promoter driving barcode 0132 and a luciferase reporter gene

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pTE5418_pMBP-GlcR

Plasmid

#221192

Purpose

Expression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.

Depositor

Tobias Erb

Article

Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

Insert

glcR (pden4400 from Paracoccus denitrificans)

Tags

10x His tag with E. coli maltose binding protein …

Expression

Bacterial

Promoter

T7 promoter and T7 promoter-lacO

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28b C7H1G6_6H N-terminal

Plasmid

#223434

Purpose

C7H1G6 GNAT expression vector with N-terminal His6 tag

Depositor

Emily Balskus

Article

Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

Insert

C7H1G6 GNAT

Tags

His6

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28b R6CZ24_6H C-terminal

Plasmid

#223435

Purpose

R6CZ24 Thiolase expression vector with C-terminal His6 tag

Depositor

Emily Balskus

Article

Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

Insert

R6CZ24 Thiolase

Tags

His6

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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T7-LacO-Halo-RGD-PILATeS (200nM, Force Inert)

Plasmid

#216717

Purpose

Expresses HaloTag-RGD-PILATeS Force Inert Control with 200 nM BiT tag in bacteria

Depositor

Alex Dunn

Article

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

HaloTag-RGD-PILATeS

Tags

6xHis and HaloTag

Expression

Bacterial

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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T7-LacO-Halo-RGD-PILATeS (700pM, Force Inert)

Plasmid

#216715

Purpose

Expresses HaloTag-RGD-PILATeS Force Inert Control with 700 pM BiT tag in bacteria

Depositor

Alex Dunn

Article

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

HaloTag-RGD-PILATeS

Tags

6xHis and HaloTag

Expression

Bacterial

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TRIF(trunc 1-183)

Plasmid

#221255

Purpose

Expression of TRIF truncation 9 (184-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 9 (184-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TRIF(trunc 1-123)

Plasmid

#221252

Purpose

Expression of TRIF truncation 6 (124-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 6 (124-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TRIF(trunc 1-143)

Plasmid

#221253

Purpose

Expression of TRIF truncation 7 (144-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 7 (144-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TRIF(trunc 1-163)

Plasmid

#221254

Purpose

Expression of TRIF truncation 8 (164-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 8 (164-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TRIF(trunc 1-43)

Plasmid

#221248

Purpose

Expression of TRIF truncation 2 (44-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 2 (44-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TRIF(trunc 1-63)

Plasmid

#221249

Purpose

Expression of TRIF truncation 3 (64-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 3 (64-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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