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pAG424-ccdB

Plasmid

#70124

Purpose

Yeast Gateway TRP1 2micron vector, no promoter (GPD1 promoter removed)

Depositor

Jay Hesselberth

Publication

Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

Type

Empty backbone

Expression

Yeast

Promoter

None

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMZ381

Plasmid

#59899

Purpose

Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469

Depositor

Mikel Zaratiegui

Publication

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Expression

Yeast

Mutation

Silent muation of the CspCI site

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pYSD001

Plasmid

#212898

Purpose

Encodes S.cerevisiae AGA2 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Publication

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

AGA2 (AGA2 Budding Yeast)

Expression

Bacterial

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD006

Plasmid

#212903

Purpose

Encodes S. cerevisiae MFalpha1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Publication

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

MFalpha1 (MF(ALPHA)1 Budding Yeast)

Expression

Bacterial

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD003

Plasmid

#212900

Purpose

Encodes S.cerevisiae ECM14 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Publication

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

ECM14 (ECM14 Budding Yeast)

Expression

Bacterial

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD063

Plasmid

#212916

Purpose

Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Publication

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

mRuby2

Expression

Bacterial

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD005

Plasmid

#212902

Purpose

Encodes S. cerevisiae KSH1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Publication

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

KSH1 (KSH1 Budding Yeast)

Expression

Bacterial

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pILGFP95

Plasmid

#83550

Purpose

Used to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Publication

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

PDA1 promoter-yEGFP

Expression

Yeast

Promoter

PDA1

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only
pILGFP86

Plasmid

#83543

Purpose

Used to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Publication

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL4A promoter-yEGFP

Expression

Yeast

Promoter

RPL4A

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFPH3

Plasmid

#83553

Purpose

Used to evaluate the expression output of HXT7 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Publication

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

HXT7 promoter-yEGFP

Expression

Yeast

Promoter

HXT7

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP84

Plasmid

#83542

Purpose

Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Publication

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

SSA1 promoter-yEGFP

Expression

Yeast

Promoter

SSA1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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YIp LYS2-HIS3

Plasmid

#122908

Purpose

LYS2-HIS3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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YIp LYS2-MET17

Plasmid

#122910

Purpose

LYS2-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-LEU2

Plasmid

#122913

Purpose

TRP1-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-MET17

Plasmid

#122914

Purpose

TRP1-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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YIp LYS2-KanMx

Plasmid

#122920

Purpose

LYS2-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pAV124

Plasmid

#63190

Purpose

pRS424 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Publication

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pACT-Lex

Plasmid

#183149

Purpose

Yeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)

Depositor

Assaf Mosquna

Publication

Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

Insert

GAL4 AD

Use

Yeat two-hybrid

Tags

HA tag

Expression

Yeast

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P81nmt1-mCFP

Plasmid

#105142

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Publication

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P81nmt1-mCFP

Tags

81nmt1-mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

81nmt1

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-mCFP

Plasmid

#105139

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Publication

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P41nmt1-mCFP

Tags

41nmt1-mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

41nmt1

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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