Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 7,234 results for: CAR

Showing: 1961 - 1980 of 7234 results

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pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG

Plasmid

#169958

Purpose

Mammalian expression of the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

Depositor

Hao Wu

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG and Ub

Expression

Mammalian

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG L368G

Plasmid

#169952

Purpose

Mammalian expression of CARD8-FLAG with a site III mutation (L368G)

Depositor

Hao Wu

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

L368G

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG E274R

Plasmid

#169950

Purpose

Mammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R)

Depositor

Hao Wu

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E274R

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG F370G

Plasmid

#169953

Purpose

Mammalian expression of CARD8-FLAG with a site III mutation (F370G)

Depositor

Hao Wu

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

F370G

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C NLRP1 UPA-CARD E1461R

Plasmid

#164007

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

Depositor

Hao Wu

Publication

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

E1461R

Available since

Feb. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C NLRP1 UPA-CARD W1460A

Plasmid

#164006

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

Depositor

Hao Wu

Publication

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

W1460A

Available since

Feb. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C NLRP1 UPA-CARD M1457A

Plasmid

#164005

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

Depositor

Hao Wu

Publication

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

M1457A

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C NLRP1 UPA-CARD Y1445A

Plasmid

#164004

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)

Depositor

Hao Wu

Publication

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

Y1445A

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-mScarlet-optoJNKi-WPRE

Plasmid

#159968

Purpose

optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

Depositor

Michael Courtney

Publication

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mScarlet-optoJNKi

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn1 CaRhAC T2A tDimer

Plasmid

#101722

Purpose

- humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons

Depositor

Thomas Oertner

Publication

Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

Inserts

CatRhAC
red fluorescent protein

Use

AAV

Mutation

E497K,C569D

Promoter

human Synapsin1 promotor and ribosomal skip seque…

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE2 (minus strand)

Plasmid

#91847

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0624)

Depositor

Jacob Corn

Publication

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE2 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

rs6602425 A>C, G>A mutation at chr10:611706…

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-CD69 CaRE1 (minus strand)

Plasmid

#91842

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0619)

Depositor

Jacob Corn

Publication

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE1 (minus strand) (CD69 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE6 (minus strand)

Plasmid

#91840

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0617)

Depositor

Jacob Corn

Publication

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE6 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

rs7893467 C>A

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE6 (plus strand)

Plasmid

#91839

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0616)

Depositor

Jacob Corn

Publication

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE6 (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

rs7893467 G>T, rs11256448 A>G

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE1 (minus strand)

Plasmid

#91838

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0615)

Depositor

Jacob Corn

Publication

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE1 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-CD69 CaRE2 (plus strand)

Plasmid

#91848

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0625)

Depositor

Jacob Corn

Publication

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE2 (plus strand) (CD69 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.23-CD69 CaRE2 (minus strand)

Plasmid

#91843

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0620)

Depositor

Jacob Corn

Publication

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE2 (minus strand) (CD69 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-HA-mScarlet3-tdP2A-scFv-GCN4-sfGFP-IRESv4-HaloTag-tdMCP

Plasmid

#241139

Purpose

Expresses Anti-HA frankenbody-mScarlet3, anti-GCN4 scfv-sfGFP, and HaloTag-tdMCP to track mature and nascent HA and SunTag-tagged proteins and MS2 tagged mRNA

Depositor

Tim Stasevich

Publication

Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

Inserts

Anti-HA frankenbody
anti-GCN4 scfv
tdMCP

Tags

HaloTag, mScarlet3, and sfGFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pBBK83 pHomL-RPL18Bp-ERG6-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179067

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of ERG6-Link-Scarlet(rfp), a marker for the lipid droplet

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-ERG6-Link-Scarlet(rfp)

Expression

Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK80 pHomL-RPL18Bp-Scarlet(rfp)-Link-VAM3-SSA1t-HomR (AmpR)

Plasmid

#179064

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VAM3, a marker for the vacuole

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-VAM3

Expression

Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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Showing: 1961 - 1980 of 7234 results

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