We narrowed to 12,354 results for: CAR

1. pMXS-IRES-BLAST flag-CAD S1859A

   Plasmid

   #169821

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   S1859A; TCCC -> AGTC silent mutations at nt527…

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. tetO-NR2F1

   Plasmid

   #170698

   Purpose

   doxycycline-inducible overexpression of NR2F1

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   nuclear receptor subfamily 2 group F member 1 (NR2F1 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   deletion of 36G- please see depositor comments

   Promoter

   TRE promoter, Tet-ON

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXPR_003-sgKCMF1

   Plasmid

   #202441

   Purpose

   Expression of a guide RNA targeting KCMF1

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   KCMF1 gRNA (KCMF1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDNA3.1 hSCN5AdeltaSIV GFP Nter

   Plasmid

   #145770

   Purpose

   expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

   Depositor

   Hugues Abriel

   Article

   Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

   Insert

   sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Stop codon at Serine 2013

   Promoter

   CMV

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPV00554 pET21GG2-His(6)-BECN(248-450)

   Plasmid

   #136685

   Purpose

   Expresses the Beclin-1 ECD/BARA domain (a.a. 248-450) with a N-terminal hexa-Histidine tag

   Depositor

   Colin Garvie

   Article

   Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

   Insert

   Beclin-1 ECD/BARA (BECN1 Human)
   

   Use

   Tags

   Hexa-Histadine

   Expression

   Bacterial

   Mutation

   DNA optimized for E. coli expression

   Promoter

   T7

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pXPR_023d-sgCiSF3B1

   Plasmid

   #202446

   Purpose

   Expression of a CRISPRi guide targeting SF3B1

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   SF3B1 gRNA (SF3B1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTRE-HRI-V5-IRES-BFP

   Plasmid

   #202435

   Purpose

   Lentiviral vector expression of HRI and TagBFP

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   HRI (EIF2AK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS L30 Flag-Apex-Rab7A

   Plasmid

   #135652

   Purpose

   Rab7A fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.

   Depositor

   Raphael Gaudin

   Article

   Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Tags

   Apex2 and FLag

   Expression

   Mammalian

   Mutation

   Promoter

   L30

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. BPV00605 pET21GG2-His(6)-BECN(1-265)

   Plasmid

   #136686

   Purpose

   Expresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tag

   Depositor

   Colin Garvie

   Article

   Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

   Insert

   Beclin-1 IDR + CC (BECN1 Human)
   

   Use

   Tags

   Hexa-Histadine

   Expression

   Bacterial

   Mutation

   DNA optimized for E. coli expression

   Promoter

   T7

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pXPR_016-sgATF4-3

    Plasmid

    #202456

    Purpose

    Expression of a guide RNA targeting ATF4

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    ATF4 gRNA (ATF4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVD1 Multiplexing Edit En-1 (GB2242)

    Plasmid

    #160564

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for the position [5_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

    Insert

    Multiplexing Edit (En-1)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pVD1 tRNA-gRNA position E2 (GB2239)

    Plasmid

    #160561

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for position [2-3] of a polycistronic tRNA-gRNA

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

    Insert

    tRNA-gRNA position E2 (Multiplexing Edit)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His

    Plasmid

    #222288

    Purpose

    Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal GFP and His tag in E.coli for purification.

    Depositor

    Anne Straube

    Article

    Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

    Insert

    KIF1C (KIF1C Human)
    

    Use

    Tags

    6xHis, GFP, and GST

    Expression

    Bacterial

    Mutation

    stalk region only amino acids 642 to 922

    Promoter

    T7

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET22b-GST-6P2-LTLT-KIF1C(642–922)-6His

    Plasmid

    #222289

    Purpose

    Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal 6xHis tag in E.coli for purification.

    Depositor

    Anne Straube

    Article

    Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

    Insert

    KIF1C (KIF1C Human)
    

    Use

    Tags

    6His and GST

    Expression

    Bacterial

    Mutation

    stalk region only amino acids 642 to 922

    Promoter

    T7

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXS-IRES-BLAST flag-CAD M1601E

    Plasmid

    #169828

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation at reported dimerization interface of the DHOase domain

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    M1601E; TCCC -> AGTC silent mutations at nt527…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMXS-IRES-BLAST flag-CAD S1406A

    Plasmid

    #169820

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported PKA phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    S1406A; TCCC -> AGTC silent mutations at nt527…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXS-IRES-BLAST flag-CAD S1859E

    Plasmid

    #169822

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    S1859E; TCCC -> AGTC silent mutations at nt527…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXPR_023d-sgCiBIRC6-1

    Plasmid

    #202447

    Purpose

    Expression of a CRISPRi guide targeting BIRC6

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    BIRC6 gRNA (BIRC6 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSITEP-shBIRC6-2(3)-C911

    Plasmid

    #202452

    Purpose

    a seed-matched control for pRSITEP-puro-shBIRC6-2(3)

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    BIRC6 shRNA (BIRC6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSITEP-shBIRC6-2(3)

    Plasmid

    #202451

    Purpose

    DOX-inducible expression of a short-hairpin RNA targetinging human BIRC6

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    BIRC6 shRNA (BIRC6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge