We narrowed to 4,483 results for: Gaa

Showing: 1961 - 1980 of 4483 results

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FUGW-H1-Scrambled control shRNA

Plasmid

#40625

Depositor

Sally Temple

Article

Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

Insert

Scrambled control shRNA

Use

Lentiviral and RNAi

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pKB01_mOrange2

Plasmid

#69102

Purpose

Expresses mOrange2 (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

Depositor

Jan-Willem Veening

Article

Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

Insert

mOrange2

Use

Synthetic Biology

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBSU6-shTim23/CMV-eGFP

Plasmid

#89795

Purpose

Expresses shRNA against TIM23 with a separate promoter for expression of GFP.

Depositor

Robert Friedlander

Article

Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

Insert

shRNA Tim23 (Timm23 Mouse)

Tags

eGFP under separate promoter

Expression

Mammalian

Promoter

U6

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_ERBB2

Plasmid

#183287

Purpose

All-in-One CRISPRko system with a guide RNA that targets ERBB2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ERBB2

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCJ1021

Plasmid

#228761

Purpose

Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

Depositor

Bradley Yoder

Insert

Ift88 gRNAs (Ift88 Mouse)

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-sgGOT1

Plasmid

#72874

Purpose

The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

sgGOT1

Use

Lentiviral

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-shNup93-XhoI

Plasmid

#87330

Purpose

To express shRNA against human Nup93

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup93

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pC0051-W85X REPAIR targeting guide cloned into pC0043

Plasmid

#103867

Purpose

Guide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

W85X targeting guide RNA

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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PX459-APP-KO

Plasmid

#176487

Purpose

To knockout APP gene

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

gRNA sequence

Use

CRISPR

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_HDAC3

Plasmid

#183299

Purpose

All-in-One CRISPRko system with a guide RNA that targets HDAC3 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

HDAC3

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSUPERretro-Sirt7 shRNA2

Plasmid

#53145

Purpose

shRNAi vector for Sirt7

Depositor

Katrin Chua

Article

Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

Insert

Sirt7

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

H1 RNA polymerase III

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only
AAV-CAG-LSL-mCherry-shPtbp1

Plasmid

#178591

Purpose

Cre-dependent expression of mCherry and a shRNA against Ptbp1 under the constitutive promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

miRE-Ptbp1

Use

AAV

Expression

Mammalian

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TLCV2-sgHPDL #3

Plasmid

#174165

Purpose

knock out HPDL in human cell lines

Depositor

Michael Pacold

Article

Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

Insert

sgRNA against HPDL

Use

Lentiviral

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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TLCV2-sgHPDL #9

Plasmid

#174166

Purpose

knock out HPDL in human cell lines

Depositor

Michael Pacold

Article

Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

Insert

sgRNA against HPDL

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pJWV25

Plasmid

#61045

Purpose

To construct N-terminal GFP fusions under control of a Zn2+ inducible promoter, integrates via double crossover at bgaA

Depositor

Jan-Willem Veening

Article

Eberhardt et al Mol Microbiol. 2009 Oct;74(2):395-408. d

Type

Empty backbone

Use

Synthetic Biology

Tags

GFP+

Expression

Bacterial

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNT3/Cre

Plasmid

#89654

Purpose

Expresses a non-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

non-targeting gRNA

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNeo2/Cre

Plasmid

#89652

Purpose

Expresses a Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Neomycin

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCK364.306

Plasmid

#192642

Purpose

Expresses dSpRY and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

J306 scRNA

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23119

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-TRAF6-targeting sgRNA 1

Plasmid

#131345

Purpose

gRNA targeting mouse TRAF6

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

gRNA targeting mouse TRAF6 (Traf6 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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gRNAy

Plasmid

#168294

Purpose

Expresses gRNA targeting the yellow gene in Drosophila

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

U6.3-gRNA[y]-scaffold

Expression

Insect

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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