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alpha catenin conformation sensor

Plasmid

#71709

Purpose

This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

Depositor

Deborah Leckband

Publication

Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

Insert

alpha-catenin FRET Sensor

Tags

ECFP and YPET

Expression

Mammalian

Promoter

CMV

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-MARK2

Plasmid

#23404

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MARK2 (MARK2 Human)

Use

Gateway Cloning

Mutation

aa1-399

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pAAV-Ef1a-DIO-H2B-GFP-2A-oG-WPRE-hGH

Plasmid

#74289

Purpose

pAAV vector to express H2B-GFP and oG (optimized Glycoprotein) in a Cre-dependent manner

Depositor

Edward Callaway

Publication

Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

Insert

H2B-GFP and oG (optimized Glycoprotein)

Use

AAV

Expression

Mammalian

Mutation

chimeric glycoprotein

Promoter

Ef1a

Available since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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GW1-Peredox-mCherry-NLS

Plasmid

#32381

Depositor

Gary Yellen

Publication

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry-NLS

Tags

Nuclear Localization Seq

Expression

Mammalian

Promoter

CMV

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pSBbi-RN

Plasmid

#60519

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and neomycin resistance gene

Depositor

Eric Kowarz

Publication

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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FlucWT-HA-GFP11-N1

Plasmid

#91954

Purpose

Expresses FlucWT-HA-GFP11 in mammalian cells

Depositor

Rong Li

Publication

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

FlucWT-HA-GFP11

Expression

Mammalian

Promoter

CMV

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-dGBP1-TagBFP

Plasmid

#80086

Purpose

GFP-dependent dGBP1-TagBFP, for expression in mammalian cells

Depositor

Connie Cepko

Publication

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

dGBP1-TagBFP

Expression

Mammalian

Promoter

CAG

Available since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pKanCMV-mClover3-18aa-actin

Plasmid

#74259

Purpose

Mammalian expression of N-terminal fusion of mClover3 to actin

Depositor

Michael Lin

Publication

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

Actin

Tags

mClover3

Expression

Mammalian

Promoter

CAG and CMV

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PTEN shRNA

Plasmid

#86645

Purpose

expression of shRNA targeting PTEN

Depositor

Jaime Modiano

Publication

Lin et al Mol Cancer. 2007 Feb 14;6:16.

Insert

PTEN (PTEN Human)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AR-V7-pcw107

Plasmid

#64635

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

AR (transcript variant 1, splice isoform) (AR Human)

Use

Lentiviral

Mutation

splice isoform*

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HOXA9 (murine) FLAG MSCV

Plasmid

#8515

Depositor

Corey Largman

Publication

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

HOXA9 (Hoxa9 Mouse)

Use

Retroviral

Tags

FLAG (DYKDDDDK) and IRES-GFP

Expression

Mammalian

Mutation

makes GFP from an IRES driving translation from a…

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pYM70

Plasmid

#119922

Purpose

CaHygB plasmids that facilitate genetic manipulations in the fungal pathogen

Depositor

Brian Wong

Publication

Basso LR et al Yeast. 2010 Dec;27(12):1039-48. doi: 10.

Insert

CaHygB

Tags

CaHygB

Promoter

TEF2

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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iRFP-C1

Plasmid

#54786

Purpose

Localization: C1 Cloning Vector, Excitation: 690, Emission: 713

Depositor

Michael Davidson, Vladislav Verkhusha

Publication

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Type

Empty backbone

Tags

iRFP

Expression

Mammalian

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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Sirius/pRSETB

Plasmid

#51956

Purpose

bacterial expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent protein

Depositor

Takeharu Nagai

Publication

Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

Insert

Sirius

Tags

6xHis

Expression

Bacterial

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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R4pGWB601_UBQ10p-miniTurbo-NES-YFP

Plasmid

#127369

Purpose

Binary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plants

Depositor

Dominique Bergmann

Publication

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Tags

GS linker, NES, V5, and YFP

Expression

Plant

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

Arabidopsis UBQ10 promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pHes1-DN-GFP (CC#110)

Plasmid

#15134

Depositor

Connie Cepko

Publication

Furukawa et al Neuron. 2000 May . 26(2):383-94.

Insert

Hes1 (Hes1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

E43A, K44A, R47A

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
nls-mCherry

Plasmid

#108881

Purpose

Expression of nuclear localized mCherry

Depositor

Rob Parton

Publication

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

nls-mCherry

Use

Can be used for mammalian expression or in vitro …

Expression

Mammalian

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pH-tdGFP

Plasmid

#74322

Purpose

pH stable tandem dimeric sfGFP variant (N149Y,Q204H) in pFA6a-URA3MX. Codon optimized for S. cerevisiae

Depositor

Joerg Stelling

Publication

Roberts et al Sci Rep. 2016 Jun 21;6:28166. doi: 10.10

Insert

pH-tdGFP

Use

Yeast tagging

Tags

none

Expression

Yeast

Mutation

N149Y, Q204H

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCDNA5-Flag-BRD4-BD

Plasmid

#90005

Purpose

expresses BD mutant BRD4

Depositor

Kornelia Polyak

Publication

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed 140N to 140A, 433N to 433A

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA

Plasmid

#47458

Purpose

LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp.

Depositor

Feng Zhang

Publication

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

TALE (Neurog2)-GS-CIB1(NLS*,∆318-334)

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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