1. pminiTol2

   Plasmid

   #31829

   Depositor

   Stephen Ekker

   Article

   Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. 1055 pminTol R4-R3

   Plasmid

   #40970

   Depositor

   Kristen Kwan

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

2. Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro

   Plasmid

   #136262

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   mNeonGreen-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Tol2 8xSTAR-sTomato-NLS. PGK-puro

   Plasmid

   #136261

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   sTomato-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Tol2 8xSTAR-mScarletI-NLS. PGK-puro

   Plasmid

   #136263

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   mScarletI-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Tol2 8xSTAR-mScarletI. PGK-puro

   Plasmid

   #136266

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   mScarletI

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Tol2 8xSTAR-sTomato. PGK-puro

   Plasmid

   #136264

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   sTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE

   Plasmid

   #168108

   Purpose

   Tol2 transposon vector of empty U6-S.aureus Cas9 gRNA cassette and CAG-driven Cre-NeoR

   Depositor

   Erich Jarvis

   Article

   Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRM27

   Plasmid

   #163695

   Purpose

   ubb promoter driven expression of lck:mNeonGreen

   Depositor

   David Matus

   Article

   Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

   Insert

   lck:mNeonGreen (lck Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   mNeonGreen

   Expression

   Mutation

   Promoter

   ubb

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Tol2 8xSTAR-mNeonGreen. PGK-puro

   Plasmid

   #136255

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Anti-Slo2.1/Slick K+ channel [N11/33R-rat IgG2a] - Chimeric

    Plasmid

    #231829

    Purpose

    Mammalian expression plasmid of anti-Slo2.1/Slick K+ channel (Mouse) rat IgG2a R-mab. Derived from hybridoma N11/33.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-Slo2.1/Slick K+ channel (Mus musculus) recombinant mouse monoclonal antibody (Kcnt2 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSL3287

    Plasmid

    #139750

    Purpose

    Base Cas12a CRISPRi plasmid

    Depositor

    Himadri Pakrasi

    Article

    Knoot et al ACS Synth Biol. 2020 Jan 17;9(1):132-143

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJBL3752

    Plasmid

    #128809

    Purpose

    Fluoride riboswitch-regulated sfGFP

    Depositor

    Julius Lucks

    Article

    Thavarajah et al ACS Synth Biol. 2019 Dec 12. doi: 10.102

    Insert

    Fluoride riboswitch-regulated sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJBL7025

    Plasmid

    #128810

    Purpose

    Fluoride riboswitch-regulated catechol 2,3-dioxygenase

    Depositor

    Julius Lucks

    Article

    Thavarajah et al ACS Synth Biol. 2019 Dec 12. doi: 10.102

    Insert

    Fluoride riboswitch-regulated catechol 2,3-dioxygenase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJBL7026

    Plasmid

    #128811

    Purpose

    Fluoride riboswitch-regulated 3-way junction dimeric broccoli

    Depositor

    Julius Lucks

    Article

    Thavarajah et al ACS Synth Biol. 2019 Dec 12. doi: 10.102

    Insert

    Fluoride riboswitch-regulated 3-way junction dimeric broccoli
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EcNGsC-MutY-pKK223

    Plasmid

    #131376

    Purpose

    Low level expression of E.coli G.stearothermophillus MutY chimera protein

    Depositor

    Martin Horvath

    Article

    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

    Insert

    EcNGsC MutY chimera
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Fusion of two MutY genes. Residues 1-225 are from…

    Promoter

    tac

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EcNGsC-F307Y-pKK223

    Plasmid

    #131381

    Purpose

    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307Y in Gs MutY.

    Depositor

    Martin Horvath

    Article

    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

    Insert

    EcNGsC MutY chimera F307Y
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Fusion of two MutY genes. Residues 1-225 are from…

    Promoter

    tac

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EcNGsC-F307A-pKK223

    Plasmid

    #131382

    Purpose

    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307A in Gs MutY.

    Depositor

    Martin Horvath

    Article

    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

    Insert

    EcNGsC MutY chimera F307A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Fusion of two MutY genes. Residues 1-225 are from…

    Promoter

    tac

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EcNGsC-S308V-pKK223

    Plasmid

    #131393

    Purpose

    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308V in Gs MutY.

    Depositor

    Martin Horvath

    Article

    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

    Insert

    EcNGsC MutY chimera S308V
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Fusion of two MutY genes. Residues 1-225 are fro…

    Promoter

    tac

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EcNGsC-S308A-pKK223

    Plasmid

    #131394

    Purpose

    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308A in Gs MutY.

    Depositor

    Martin Horvath

    Article

    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

    Insert

    EcNGsC MutY chimera S308A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Fusion of two MutY genes. Residues 1-225 are fro…

    Promoter

    tac

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge