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PX459 mDnd1#1

Plasmid

#106345

Purpose

small guide RNA #1 against RRM-coding region of Dnd1 gene locus

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
PX459 mDnd1#2

Plasmid

#106346

Purpose

small guide RNA #2 against RRM-coding region of Dnd1 gene locus

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
PX459 mDnd1#3

Plasmid

#106347

Purpose

small guide RNA #3 against RRM-coding region of Dnd1 gene locus

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR4-hDND1

Plasmid

#70070

Purpose

Gateway entry vector for human DND1 with stop codon

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (DND1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVE-FH-Dnd1-IN

Plasmid

#70072

Purpose

2nd gen lentiviral vector. Cre-removable and tet/dox-controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (Dnd1 Mouse)

Use

Cre/Lox and Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVCT-Dnd1-EGFP

Plasmid

#106343

Purpose

Lentiviral vector, mammalian expression of Dnd1-EGFP fusion protein

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1-EGFP (Dnd1 Mouse, Synthetic)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-shDnd1-No1

Plasmid

#70058

Purpose

TRC lentiviral vector for shRNA against mouse Dnd1 #1

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (Dnd1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-shDnd1-No3

Plasmid

#70060

Purpose

TRC lentiviral vector for shRNA against mouse Dnd1 #3

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (Dnd1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-shDnd1-No4

Plasmid

#70065

Purpose

TRC lentiviral vector for shRNA against mouse Dnd1 #4

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (Dnd1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only
pLKO.1-shDnd1-No5

Plasmid

#70066

Purpose

TRC lentiviral vector for shRNA against mouse Dnd1 #5

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (Dnd1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-shDnd1-No6

Plasmid

#70067

Purpose

TRC lentiviral vector for shRNA against mouse Dnd1 #6

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (Dnd1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only
pLKO.1-shDnd1-No7

Plasmid

#70068

Purpose

TRC lentiviral vector for shRNA against mouse Dnd1 #7

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (Dnd1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only
pLKO.1-shDnd1-No8

Plasmid

#70069

Purpose

TRC lentiviral vector for shRNA against mouse Dnd1 #8

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (Dnd1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only
pET11d-FHis-DND1

Plasmid

#70083

Purpose

Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein. Codons are optimized for bacteria expression.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (DND1 Human)

Use

Tags

6xHIS and FLAG

Expression

Bacterial

Mutation

Codon optimized for bacterial expression

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pET11d-FHis-DND1-Y102A

Plasmid

#70082

Purpose

Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein with defective RRM (Y102A). Codons are optimized for bacteria expression.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (DND1 Human)

Use

Tags

6xHIS and FLAG

Expression

Bacterial

Mutation

Y102A. Codon optimized for bacterial expression

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS-TRE-FHDnd1-Ubc-rtTA-I2G

Plasmid

#70076

Purpose

2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (Dnd1 Mouse)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Silent mutations are introduced to escape from RN…

Promoter

Tet responsible promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G

Plasmid

#70075

Purpose

2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 (RRM mutant) in mammalian cells

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (Dnd1 Mouse)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Y102A. Silent mutations are introduced to escape …

Promoter

Tet responsible promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TO-DEST

Plasmid

#106348

Purpose

Destination vector for gateway cloning. Developed for Flp-In T-REx system.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Type

Empty backbone

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pBRPB CAG-mCherry-IP

Plasmid

#106333

Purpose

PiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

mCherry-IRES-Puro

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR4-EGFP_opt

Plasmid

#106334

Purpose

Gateway Entry vector for EGFP (codon optimized for efficient gene synthesis)

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

EGFP

Use

Gateway entry vector

Tags

Expression

Bacterial and Mammalian

Mutation

codon optimized for gene synthesis without affect…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge