Showing: 21 - 36 of 36 results
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Plasmid#208619PurposeMammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.DepositorInsertmsEGFP1.8
UseTagsExpressionMammalianMutation(compared with pEGFP-C1, protein level) S30R, Y39…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET28-mEGFP(A206K)-TwStrep
Plasmid#208622Purposefor E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) cloneDepositorInsertmEGFP(A206K)
UseTags6xHis and Twin StrepExpressionBacterialMutation(compared with pEGFP-C1, protein level) A206KPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pUC-T7-J1~398-Luc
Plasmid#208639Purposefor in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19DepositorInsertHepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
UseLuciferase; In vitro transcription templateTagsFirefly Luciferase (F-Luc and T7 promoterExpressionMutationnt 1~398, in frame with donstream F-LucPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pUC-ODN-hsRPL3-CterTwStrep
Plasmid#208644Purposefor long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.DepositorInsertTwin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
UseCRISPR; Ssdna donnor preparationTagsTwin StrepExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro-sgRPL3-56
Plasmid#208979PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6 in tandemAvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-1~77-mEGFP(A206K)-TwStrep
Plasmid#208624Purposefor E. coli expression truncated human RPL3 (ENST00000216146), aa1~77, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 1~77 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 1~77, other residues not includedPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-72~197-mEGFP(A206K)-TwStrep
Plasmid#208625Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa72~197, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 72~197 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 72~197, other residues not includedPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-191~307-mEGFP(A206K)-TwStrep
Plasmid#208626Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 191~307, other residues not includedPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-299~398-mEGFP(A206K)-TwStrep
Plasmid#208627Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 299~398, other residues not includedPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-1~41-mEGFP(A206K)-TwStrep
Plasmid#208628Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 1~41, other residues not includedPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-212~285-mEGFP(A206K)-TwStrep
Plasmid#208629Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 212~285, other residues not includedPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-Δ1~34,Δ215~284-TwStrep
Plasmid#208630Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.DepositorInserthuman RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationΔ1~34,Δ215~284PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-53~77-mEGFP(A206K)-TwStrep
Plasmid#208632Purposefor E. coli expression truncated human RPL3 (ENST00000216146), aa53~77, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 53~77 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 53~77, other residues not includedPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pUC-T7-hTP53-P0~670-FLuc
Plasmid#208640Purposefor in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.DepositorInsertTP53, UTR and partial CDS (TP53 Human)
UseLuciferase; In vitor transcription templateTagsFirefly Luciferase (F-Luc) and T7 promoterExpressionMutationUTR and partial CDS in-frame with F-LucPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro-sgRPL3-62
Plasmid#208647PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Human, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6 in tandemAvailabilityAcademic Institutions and Nonprofits only -
pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56
Plasmid#208645PurposeRemove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.DepositorInsertTwin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
UseCRISPR; Ssdna donnor preparation for knock-in of…TagsTwin StrepExpressionMutationTwo SNP (rs749555789, rs760194863) were introduce…PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 21 - 36 of 36 results