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We narrowed to 93 results for: aav gfp cre

Showing: 1 - 10 of 93 results

  1. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ... 50363  AAV5  AAV phSyn1(S)-DreO-bGHpA 121675  AAV9  pAAV-EF1a-fDIO-Cre (FLP-dependent CRE) 55634...constitutive (pAAV-Syn-Archon1-KGC-GFP-ER2) and cre-dependent version (pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2])...pAAV.hSyn-FLEX.iGABASnFR.F102G pAAV-Syn-Archon1-KGC-GFP-ER2 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] pAAV-FLEX-tdTomato pAAV-GFAP104...Our new AAVs include: pGP-AAV-syn-FLEX-jGCaMP7b-WPRE pAAV.hSyn-FLEX.iGABASnFR pAAV.hSyn-FLEX.iGABASnFR.... of jGCaMP7 in AAV1 at Addgene! See all calcium sensor AAV at Addgene. GABA sensor AAV GABA is the primary...diversified) 37825  AAV8*, AAV9*  pAAV-CAG-GFP 50465  AAV8*, AAV9*  pAAV-hSyn-EGFP *Also coming in...are some of the AAV we have released so far in 2019. You can also browse our entire AAV inventory! Our ...

  2. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...expressing both Cre and GFP. The GFP dependent FLPo, Flp-DOG (75469), is also available in AAV1. Plasmid...respectively of a GFP dependent Cre. The two segments come together in the presence of GFP to form a functional...genome-wide inhibition screening. Find the Dolcetto lentiviral prep here Control AAV Control AAV allow researchers...encoding a Cre dependent version of the DREADD hM3D is now also offered as AAV1 (along with AAV PHP.eB, ...proteins ChR2 for targeted expression DREADDs GFP dependent Cre and FLPo And more!  New lentiviral preps...offer control AAV in smaller aliquots of 20ul for serotype testing to help you pick the best AAV for your ...plasmids in new serotypes, as well as a new Cre dependent GFP control plasmid for expression in forebrain...

  3. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...efficiency of AAV-PHP.eB and AAV-PHP.S versus their parental AAV vectors. What can these AAV’s be used for...efficiency of AAV-PHP.B versus AAV-PHP.eB in the CNS. A GFP transgene was packaged into either an AAV-PHP.B or...Search for AAV Vectors to use with your research. Visit Our AAV Guide Page for info on AAV components,...method?  CREATE stands for Cre Recombinase-based AAV Targeted Evolution. In this technique, a pool of mutant...cell-type-specific Cre, the polyA sequence is inverted only in the Cre-expressing tissues. This inversion creates a template...neurons and astrocytes. Why screen for IV deliverable AAVs? Typically AAVs are directly injected into ...PNS. A GFP transgene was packaged into either an AAV.9 or an AAV.PHP.S capsid. Both viruses were IV injected...

  4. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...channelrhodopsin, fused to a fluorescent GFP marker (CatCH-GFP). The lab deposited AAV plasmids containing the active... of Cre-mCherry and a second promoter to drive expression of a Cre-dependent fluorescent CatCH-GFP reporter...Expression of GFP will only result in cells where Cre-mCherry is also expressed. If Cre-mCherry expresses...horizontal cells transduced by both AAVs would express Cre-mCherry and GFP.   Figure 2: Synthetic promoters...Performance of synthetic promoter AAVs in mice All of these synthetic promoter AAVs were first injected into the...  Applications for synthetic promoter AAVs Besides restricting AAV transgene expression to particular cell... photoreceptors and horizontal cells, and Cre-dependent-GFP expresses in horizontal cells and ganglion...

  5. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...popular control AAV encoding GFP. Check out our inventory of serotype testing AAV New control AAVs Plasmid...Flp-expressing AAV, as well as a Cre-dependent Flp-expressing AAV. See our recombinase AAV inventory here...testing) pAAV-CAG-GFP 50465 AAV8, 9 (20 µL size serotype testing) AAV-hSyn-EGFP Biosensor AAV The ability...Serotype Name 104496  AAV1 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE 105322  AAV1 pGP-AAV-syn-FLEX-jGCaMP7c variant...Name 119741  AAV5  AAV SYN flex PSAM4 GlyR IRES EGFP Recombinase AAV Finally, we are excited to add...some of the AAV we have released in the last few months. You can also browse our entire AAV inventory. .... Our new AAVs include: Control AAVs for serotype testing jGCaMP7c for calcium detection PSAM4 GlyR for...

  6. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...neuronal tracing Retrograde AAV: Making the journey from axon to nucleus AAVs CREATEd for gene delivery to the...immunogenicity, and the long-term expression of AAV delivered genes. AAV serotypes have been extensively studied... structures GFP is limited in its ability to label subcellular structures in the CNS. GFP and other FPs...Monster probes at Addgene Browse all AAV vectors Find ready-to-use AAV preps ...photostable than GFP. In addition, antibody-based amplification of their signals is weak and increases background...three decades, recombinant Adeno-associated virus (AAV) has been widely used to deliver genetic tools to...atlas enables researchers to quickly find the best AAV serotypes to target the neurons they’re studying....

  7. Viral Production at Addgene

    Type
    Blog Post
    ...Two days later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected...Figure 2: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at ... the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from... and quality control are described below. AAV Production AAV distributed by Addgene has been produced ...purity of the AAV preparation. Figure 1: Silver staining of purified and non-purified AAV subjected...arrowhead). Scale bar = 100 nm.   AAV resources Browse AAV preps available from Addgene. After receiving...Addgene blog Find and share AAV Data with Addgene's Data Hub Digital Droplet for AAV Quantification Viral Vectors...

  8. Hot Plasmids - May 2022

    Type
    Blog Post
    ...labeling Increased neuronal expression using Cre-mediated AAV Targeted Evolution method (M-CREATE) dCas9...-4.   Increased neuronal expression using Cre-mediated AAV Targeted Evolution method (M-CREATE) by: Brian...multiplexed version of the Cre-mediated AAV Targeted Evolution method (M-CREATE), in which one applies both... of the AAV capsids (left) and transduction patterns (right).  Both AAV-PHP.eB and the AAV.CAP-B capsids...line expressing a GFP-tagged version of a protein of interest. The binding of the GFP nanobody to its target... proximity to the GFP-tagged protein (albeit slightly offset due to the nanobody-GFP interaction). The...ligase TurboID fused to a conditionally stabilized GFP-nanobody rather than a specific protein of interest...

  9. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...pAAV-EF1a-fDIO-Cre AAV5 Recombinases Esteban Engel New serotype pAAV-hSyn-Cre-P2A-dTomato AAV PHP.eB Recombinases...viral prep pAAV-CAG-GFP AAV9-X1.1 Controls Edward Boyden New serotype pAAV-hSyn-EGFP AAV PHP.eB Controls...levels compared to AAV9. You can now target these cells with AAV-CAG-GFP packaged in AAV9-X1.1!  We also ...Notes AAV-GfaABC1D-Cre-4x6T AAV5 Recombinases Stanley Thomas Carmichael New viral prep pAAV-EF1a-fDIO-Cre...serotype AiP13778 - pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) AAV PHP.eB Recombinases Jonathan...viral prep AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) AAV PHP.eB Recombinases...viral prep AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) AAV PHP.eB Recombinases Jonathan...

  10. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...GBPs/GFP. The new system, CRE-DOG (Cre Dependent On GFP), is activated by GFP and derivatives GFP and ...Connie Cepko Combining Cre and GFP with Cre-DOGs The T-DDOG results showed that GFP could regulate transcription...transcription or Cre recombinase activity only in the presence of GFP. These systems, known as T-DDOG and Cre-DOG,...to bind GFP. The idea of using GFP as a scaffold suddenly seemed very realistic. Envisioning GFP as a substrate...induced in ~76% of GFP+ cells, and 100% of DsRed+ cells also expressed GFP. Using AAV constructs, Tang ... cord. Cre-DOG is also suitable for optogenetic studies, and since cells infected with AAV-Cre-DOG retain...cells cotransfected with GFP and the GBPs, GFP linked the two GBPs together to create a complete transcription...
Showing: 1 - 10 of 93 results